Abstract
The use of cereal microgreens is increasing because of increased consumer’s interest in healthier products. Chlorophyll (Chl) and Carotenoids (Car) are suggested to correlate with health promoting components like phenolics and antioxidant potential of the plant-part. They also play role against clinical conditions like thalassemia and hemolytic anemia and reduce the risk of some chronic diseases, such as cancer, cardiovascular diseases, skin diseases and age-related eye diseases. This study was carried out for the comprehensive profiling of Chl and Car in wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) micro-greens between 7 and 16 days on dry basis. Chl and Car content strongly correlated with the number of days of growth. Significantly high correlations existed among Chl a, Chl b, total Chl and total Car with concomitant Chl a/b and Chl/Car ratios. The peaks for the rate of accumulation of pigments were between 7–10 days on wheat and 10–13 days on barley. The maximum content of Chl and Car were 616.63 ± 18.45 mg/100 g DM and 54.80 ± 1.72 mg/100 g DM on day 16 and statistically not significant to variety of grain. The Chl level was slightly lower than Chl rich vegetables like kale and comparable to spinach and Car level was comparable to carrots, which is higher than most of the daily consumable fruits and vegetables. Further cell-based or in vivo studies of cereal microgreens could be considered to draw more valuable information related to human health.
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References
Battersby AR (1988) Biosynthesis of the pigments of life. J Nat Prod 51:629–642
Beck WA, Redman R (1940) Seasonal variations in the production of plant pigments. Plant Physiol 15:81–94
Benincasa P, Galieni A, Anna CM, Pace R, Guiducci M, Pisante M, Stagnari F (2015) Phenolic compounds in grains, sprouts and wheatgrass of hulled and non-hulled wheat species. J Sci Food Agric 95:1795–1803
Chen K, Roca M (2018) In vitro bioavailability of chlorophyll pigments from edible seaweeds. J Funct Foods 41:25–33
During A, Doraiswamy S, Harrison EH (2008) Xanthophylls are preferentially taken up compared with b-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res 49:1715–1724
Eldahshan OA, Singab ANB (2013) Carotenoids. J Pharmacogn Phytochem 2:225–234
Farnham MW, Kopsell DA (2009) Importance of genotype on carotenoid and chlorophyll levels in broccoli heads. HortScience 44:1248–1253
Filimon RV, Rotaru L, Filimon RM (2016) Quantitative investigation of leaf photosynthetic pigments during annual biological cycle of Vitis vinifera L. table grape cultivars. S Afr J Enol Vitic 37:1–14
Gedi MA, Briars R, Yuseli F, Zainol N, Darwish R, Salter AM, Gray DA (2017) Component analysis of nutritionally rich chloroplasts: recovery from conventional and unconventional green plant species. J Food Sci Technol 54:2746–2757
Haldimann P (1999) How do changes in temperature during growth affect leaf pigment composition and photosynthesis in Zea mays genotypes differing in sensitivity to low temperature? J Exp Bot 50:543–550
Holm G (1954) Chlorophyll mutations in barley. Acta Agric Scand 4:457–471
Januskaitiene I, Klepeckas M (2015) The effect of equal Cd and Cu exposure in peat substrate on growth and bioaccumulation of Hordeum vulgare. Biologija 61:83–93
Kohler GO (1944) The effect of stage of growth on the chemistry of the grasses. J Biol Chem 152:215–223
Kopsell DA, Kopsell DE, Curran-Celentano J (2005) Carotenoid and chlorophyll pigments in sweet basil grown in the field and greenhouse. HortScience 40:1230–1233
Lefsrud M, Kopsell D, Wenzel A, Sheehan J (2007) Changes in kale (Brassica oleracea L. var. acephala) carotenoid and chlorophyll pigment concentrations during leaf ontogeny. Sci Hortic 112:136–141
Lichtenthaler HK (2007) Biosynthesis, accumulation and emission of carotenoids, a -tocopherol, plastoquinone, and isoprene in leaves under high photosynthetic irradiance. Photosynth Res 92:163–179
Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592
Meng FL, Xv YY, Hu JD, He D, Jiang JH (2017) Optimization of combined drying process by hot-air and microwave for barley seedling powder. J Food Saf Qual 8:1651–1658
Ndukwe OK, Edeoga HO, Okwulehie IC, Omosun G (2016) Variability in the Chlorophyll and Carotene Composition of ten maize (Zea mays) varieties. Eur J Phys Agric Sci 4:1–6
Niroula A, Khatri S, Khadka D, Timilsina R (2019) Total phenolic contents and antioxidant activity profile of selected cereal sprouts and grasses. Int J Food Prop 22:427–437
Okpalanma FE, Ojimelukwe PC (2018) Evaluation of effects of storage condition and processing on carotenoids, chlorophyll, vitamins and minerals in a water leaf (Talinum triangulare). Asian Food Sci J 2:41603
Ozkose A, Arslan D, Acar A (2016) The comparison of the chemical composition, sensory, phenolic and antioxidant properties of juices from different wheatgrass and turfgrass species. Not Bot Horti Agrobot Cluj-Napoca 44:499–507
Padalia S, Drabu S, Raheja I, Gupta A, Dhamija M (2010) Multitude potential of wheatgrass juice (Green Blood): an overview. Chron Young Sci 1:23–28
Paznocht L, Kotíková Z, Miloslav Š, Lachman J, Orsák M, Eliášová M, Martinek P (2018) Free and esterified carotenoids in pigmented wheat, tritordeum and barley grains. Food Chem 240:670–678
Rosevear MJ, Young AJ, Johnson GN (2001) Growth conditions are more important than species origin in determining leaf pigment content of British plant species. Funct Ecol 15:474–480
Samanta T, Kotamreddy JNR, Ghosh BC, Mitra A (2017) Changes in targeted metabolites, enzyme activities and transcripts at different developmental stages of tea leaves: a study for understanding the biochemical basis of tea shoot plucking. Acta Physiol Plant 39:11
Shah SH, Houborg R, Mccabe MF (2017) Response of chlorophyll, carotenoid and SPAD-502 measurement to salinity and nutrient stress in wheat (Triticum aestivum L.). Agronomy 7:61
Simova-stoilova L, Stoyanova Z, Demirevska-kepova K (2001) Ontogenic changes in leaf pigments, total soluble proteins and Rubisco in two barley varieties in relation to yield. Bulgerian J Plant Physiol 27:15–24
Siriwatanametanon N (2017) Warfarin-chlorophyll products, herb-drug interactions. Pharm Sci Asia 44:173–189
Skoczylas L, Korus A, Tabaszewska M, Gedos K, Szczepanska E (2018) Evaluation of the quality of fresh and frozen wheatgrass juices depending on the time of grass harvest. J Food Process Preserv 43:1–8
Wakeham P (2013) The medicinal and pharmacological screening of wheatgrass juice (Triticum aestivum L.): an investigation into chlorophyll content and antimicrobial activity. Plymouth Stud Sci 6:20–30
Wolf FT (1963) Effects of light and darkness on biosynthesis of carotenoid pigments in wheat seedlings. Plant Physiol 38:649–652
Yang CM, Lee CN, Chou CH (2002) Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: I. Inhibition of supply-orientation. Bot Bull Academia Sinica 43:299–324
Yoon GA, Yeum KJ, Cho YS, Chen CYO, Tang G, Blumberg JB, Russell RM, Yoon S, Lee-Kim YC (2012) Carotenoids and total phenolic contents in plant foods commonly consumed in Korea. Nutr Res Pract 6:481–490
Zaripheh S, Erdman JW (2002) Factors that influence the bioavailablity of xanthophylls. In: Can lutein protect against chronic disease? pp S531–S534
Znidarcic D, Ban D, Šircelj H (2011) Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. Food Chem 129:1164–1168
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Authors would like to thank National College of Food Science and Technology (NCFST), Tribhuvan University, Nepal for the Internal grant.
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Niroula, A., Khatri, S., Timilsina, R. et al. Profile of chlorophylls and carotenoids of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) microgreens. J Food Sci Technol 56, 2758–2763 (2019). https://doi.org/10.1007/s13197-019-03768-9
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DOI: https://doi.org/10.1007/s13197-019-03768-9