Abstract
The antifungal effect of Lactobacillus plantarum C10 on pink rot caused by Trichothecium roseum and its application in muskmelon fruit were investigated. Cell-free supernatant (CFS) produced by Lactobacillus plantarum C10 strongly inhibited the growth of T. roseum and seriously damaged the structures of spores and mycelia of T. roseum. Acid compounds produced by Lb. plantarum C10 were the major antifungal substances and exhibited a narrow pH range from 3.5 to 6.5. Application of the CFS on muskmelon fruit reduced the contamination zone of T. roseum by enhancing the activities of defensive enzymes (phenylalanine ammonialyase, peroxidase and polyphenoloxidase) and promoting the accumulation of phenolics and flavonoids. These results suggested that Lb. plantarum C10 could be used as a biocontrol agent to control pink rot caused by T. roseum in muskmelon fruit.
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References
Brisson LF, Tenhaken R, Lamb C (1995) Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. Plant Cell 6:1703–1712. https://doi.org/10.1105/tpc.6.12.1703
Cheong EYL et al (2014) Isolation of lactic acid bacteria with antifungal activity against the common cheese spoilage mould Penicillium commune and their potential as biopreservatives in cheese. Food Control 46:91–97. https://doi.org/10.1016/j.foodcont.2014.05.011
Crowley S, Mahony J, van Sinderen D (2013a) Broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models. Folia Microbiol 58:291–299. https://doi.org/10.1007/s12223-012-0209-3
Crowley S, Mahony J, van Sinderen D (2013b) Current perspectives on antifungal lactic acid bacteria as natural bio-preservatives. Trends Food Sci Technol 33:93–109. https://doi.org/10.1016/j.tifs.2013.07.004
Deng J et al (2015) Postharvest oxalic acid treatment induces resistance against pink rot by priming in muskmelon (Cucumis melo L.) fruit. Postharvest Biol Technol 106:53–61. https://doi.org/10.1016/j.postharvbio.2015.04.005
Frankova A et al (2016) The antifungal activity of essential oils in combination with warm air flow against postharvest phytopathogenic fungi in apples. Food Control 68:62–68. https://doi.org/10.1016/j.foodcont.2016.03.024
Galvez A, Abriouel H, Benomar N, Lucas R (2010) Microbial antagonists to food-borne pathogens and biocontrol. Curr Opin Biotechnol 21:142–148. https://doi.org/10.1016/j.copbio.2010.01.005
Ge Y, Deng H, Bi Y, Li C, Liu Y, Dong B (2015) Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit. Postharvest Biol Technol 99:160–167. https://doi.org/10.1016/j.postharvbio.2014.09.001
Gerez CL, Torres MJ, Font de Valdez G, Rollán G (2013) Control of spoilage fungi by lactic acid bacteria. Biol Control 64:231–237. https://doi.org/10.1016/j.biocontrol.2012.10.009
Ghosh R, Barman S, Mukhopadhyay A, Mandal NC (2015) Biological control of fruit-rot of jackfruit by rhizobacteria and food grade lactic acid bacteria. Biol Control 83:29–36. https://doi.org/10.1016/j.biocontrol.2014.12.020
Konappa NM, Maria M, Uzma F, Krishnamurthy S, Nayaka SC, Niranjana SR, Chowdappa S (2016) Lactic acid bacteria mediated induction of defense enzymes to enhance the resistance in tomato against Ralstonia solanacearum causing bacterial wilt. Sci Hortic 207:183–192. https://doi.org/10.1016/j.scienta.2016.05.029
Lan WT, Chen YS, Wu HC, Yanagida F (2012) Bio-protective potential of lactic acid bacteria isolated from fermented wax gourd. Folia Microbiol 57:99–105. https://doi.org/10.1007/s12223-012-0101-1
Lavermicocca P, Valerio F, Evidente A, Lazzaroni S, Corsetti A, Gobbetti M (2000) Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl Environ Microbiol 66:4084–4090. https://doi.org/10.1128/aem.66.9.4084-4090.2000
Li H et al (2014) Antifungal activities and effect of Lactobacillus casei AST18 on the mycelia morphology and ultrastructure of Penicillium chrysogenum. Food Control 43:57–64. https://doi.org/10.1016/j.foodcont.2014.02.045
Lu L et al (2013) Rhodosporidium paludigenum induces resistance and defense-related responses against Penicillium digitatum in citrus fruit. Postharvest Biol Technol 85:196–202. https://doi.org/10.1016/j.postharvbio.2013.06.014
Mohammadi M, Kazemi H (2002) Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Sci 162:491–498. https://doi.org/10.1016/S0168-9452(01)00538-6
Passardi F, Penel C, Dunand C (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9:534–540. https://doi.org/10.1016/j.tplants.2004.09.002
Pawlowska AM, Zannini E, Coffey A, Arendt EK (2012) “Green preservatives”: combating fungi in the food and feed industry by applying antifungal lactic acid bacteria. Adv Food Nutr Res 66:217–238. https://doi.org/10.1016/B978-0-12-394597-6.00005-7
Plaza L, Altisent R, Alegre I, Viñas I, Abadias M (2016) Changes in the quality and antioxidant properties of fresh-cut melon treated with the biopreservative culture Pseudomonas graminis CPA-7 during refrigerated storage. Postharvest Biol Technol 111:25–30. https://doi.org/10.1016/j.postharvbio.2015.07.023
Prema P, Smila D, Palavesam A, Immanuel G (2008) Production and characterization of an antifungal compound (3-phenyllactic acid) produced by Lactobacillus plantarum strain. Food Bioprocess Technol 3:379–386. https://doi.org/10.1007/s11947-008-0127-1
Sivankalyani V, Feygenberg O, Diskin S, Wright B, Alkan N (2016) Increased anthocyanin and flavonoids in mango fruit peel are associated with cold and pathogen resistance. Postharvest Biol Technol 111:132–139. https://doi.org/10.1016/j.postharvbio.2015.08.001
Terry L (2004) Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biol Technol 32:1–13. https://doi.org/10.1016/j.postharvbio.2003.09.016
Torres R, Teixidó N, Usall J, Abadias M, Mir N, Larrigaudiere C, Viñas I (2011) Anti-oxidant activity of oranges after infection with the pathogen Penicillium digitatum or treatment with the biocontrol agent Pantoea agglomerans CPA-2. Biol Control 57:103–109. https://doi.org/10.1016/j.biocontrol.2011.01.006
Tripathi P, Dubey NK (2004) Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biol Technol 32:235–245. https://doi.org/10.1016/j.postharvbio.2003.11.005
Tsuda K et al (2016) Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions. Biol Control 100:63–69. https://doi.org/10.1016/j.biocontrol.2016.05.010
Wang H, Yan H, Shin J, Huang L, Zhang H, Qi W (2011) Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China. Ann Microbiol 61:879–885. https://doi.org/10.1007/s13213-011-0209-6
Wang H et al (2013a) Genome shuffling of Lactobacillus plantarum for improving antifungal activity. Food Control 32:341–347. https://doi.org/10.1016/j.foodcont.2012.12.020
Wang J, Bi Y, Wang Y, Deng J, Zhang H, Zhang Z (2013b) Multiple preharvest treatments with harpin reduce postharvest disease and maintain quality in muskmelon fruit (cv. Huanghemi). Phytoparasitica 42:155–163. https://doi.org/10.1007/s12600-013-0351-8
Wang X, Xu F, Wang J, Jin P, Zheng Y (2013c) Bacillus cereus AR156 induces resistance against Rhizopus rot through priming of defense responses in peach fruit. Food Chem 136:400–406. https://doi.org/10.1016/j.foodchem.2012.09.032
Yan Y et al (2018) Control of postharvest blue mold decay in pears by Meyerozyma guilliermondii and it’s effects on the protein expression profile of pears. Postharvest Biol Technol 136:124–131. https://doi.org/10.1016/j.postharvbio.2017.10.016
Yang EJ, Chang HC (2010) Purification of a new antifungal compound produced by Lactobacillus plantarum AF1 isolated from kimchi. Int J Food Microbiol 139:56–63. https://doi.org/10.1016/j.ijfoodmicro.2010.02.012
Yang B, Yongcai L, Yonghong G, Yi W (2009) Induced resistance in melons by elicitors for the control of postharvest diseases. Postharvest Pathol 2:31–41. https://doi.org/10.1007/978-1-4020-8930-5_3
Yi W, Xuan LI, Yang BI, Yong-Hong GE, Yong-Cai LI, Fang X (2008) Postharvest ASM or harpin treatment induce resistance of muskmelons against Trichothecium roseum. Agric Sci China 7:217–223. https://doi.org/10.1016/S1671-2927(08)60042-5
Zhang Z, Bi Y, Ge Y, Wang J, Deng J, Xie D, Wang Y (2011) Multiple pre-harvest treatments with acibenzolar-S-methyl reduce latent infection and induce resistance in muskmelon fruit. Sci Hortic 130:126–132. https://doi.org/10.1016/j.scienta.2011.06.024
Zhang Q et al (2016) Streptomyces rochei A-1 induces resistance and defense-related responses against Botryosphaeria dothidea in apple fruit during storage. Postharvest Biol Technol 115:30–37. https://doi.org/10.1016/j.postharvbio.2015.12.013
Acknowledgements
This study was funded by the Research Project from Science and Technology Department of Liaoning Province of China (No. 2015103020), and the Team Support Program for the Taishan Scholar of Blue Industry leading personnel of Shandong Province of China (LZBZ2015-19).
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Lv, X., Ma, H., Lin, Y. et al. Antifungal activity of Lactobacillus plantarum C10 against Trichothecium roseum and its application in promotion of defense responses in muskmelon (Cucumis melo L.) fruit. J Food Sci Technol 55, 3703–3711 (2018). https://doi.org/10.1007/s13197-018-3300-1
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DOI: https://doi.org/10.1007/s13197-018-3300-1