Optimization of microwave assisted extraction of essential oils from Iranian Rosmarinus officinalis L. using RSM

  • Maryam Akhbari
  • Saeed Masoum
  • Fahimeh Aghababaei
  • Sepideh Hamedi
Original Article
  • 13 Downloads

Abstract

In this study, the efficiencies of conventional hydro-distillation and novel microwave hydro-distillation methods in extraction of essential oil from Rosemary officinalis leaves have been compared. In order to attain the best yield and also highest quality of the essential oil in the microwave assisted method, the optimal values of operating parameters such as extraction time, microwave irradiation power and water volume to plant mass ratio were investigated using central composite design under response surface methodology. Optimal conditions for obtaining the maximum extraction yield in the microwave assisted method were predicted as follows: extraction time of 85 min, microwave power of 888 W, and water volume to plant mass ratio of 0.5 ml/g. The extraction yield at these predicted conditions was computed as 0.7756%. The qualities of the obtained essential oils under designed experiments were optimized based on total contents of four major compounds (α-pinene, 1,8-cineole, camphor and verbenone) which determined by gas chromatography equipped with mass spectroscopy (GC–MS). The highest essential oil quality (55.87%) was obtained at extraction time of 68 min; microwave irradiation power of 700 W; and water volume to plant mass ratio of zero.

Keywords

Rosemary officinalis L. Quality assessment of essential oil Gas chromatography–mass spectroscopy Response surface methodology Central composite design 

Notes

Acknowledgements

The authors would like to thank University of Kashan for its financial supports.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

References

  1. Akhbari M, Yaghoobei M, Hamedi S (2017) Composition of the oily compounds, phytochemical screening and biological activity of different aerial parts of Smirnovia turkestana Bunge. Nat Prod Res.  https://doi.org/10.1080/14786419.2017.1374263 Google Scholar
  2. Akhbari M, Kord R, Jafari Nodooshan S, Hamedi S (2018) Analysis and evaluation of the antimicrobial and anticancer activities of the essential oil isolated from Foeniculum vulgare from Hamedan, Iran. Nat Prod Res.  https://doi.org/10.1080/14786419.2017.1423310 Google Scholar
  3. Babuskin S et al (2015) Effects of Rosemary extracts on oxidative stability of chikkis fortified with microalgae biomass. J Food Sci Technol 52:3784–3793.  https://doi.org/10.1007/s13197-014-1439-y Google Scholar
  4. Bellumori M, Innocenti M, Binello A, Boffa L, Mulinacci N, Cravotto G (2016) Selective recovery of rosmarinic and carnosic acids from rosemary leaves under ultrasound and microwave-assisted extraction procedures. C R Chim 19:699–706.  https://doi.org/10.1016/j.crci.2015.12.013 CrossRefGoogle Scholar
  5. Bousbia N, Abert Vian M, Ferhat MA, Petitcolas E, Meklati BY, Chemat F (2009) Comparison of two isolation methods for essential oil from rosemary leaves: hydrodistillation and microwave hydrodiffusion and gravity. Food Chem 114:355–362.  https://doi.org/10.1016/j.foodchem.2008.09.106 CrossRefGoogle Scholar
  6. Bustamante J et al (2016) Microwave assisted hydro-distillation of essential oils from wet citrus peel waste. J Clean Prod 137:598–605.  https://doi.org/10.1016/j.jclepro.2016.07.108 CrossRefGoogle Scholar
  7. Chen F, Du X, Zu Y, Yang L, Wang F (2016) Microwave-assisted method for distillation and dual extraction in obtaining essential oil, proanthocyanidins and polysaccharides by one-pot process from Cinnamomi Cortex. Sep Purif Technol 164:1–11.  https://doi.org/10.1016/j.seppur.2016.03.018 CrossRefGoogle Scholar
  8. Chen F, Xu M, Yang X, Liu J, Xiao Y, Yang L (2018) An improved approach for the isolation of essential oil from the leaves of Cinnamomum longepaniculatum using microwave-assisted hydrodistillation concatenated double-column liquid-liquid extraction. Sep Purif Technol 195:110–120.  https://doi.org/10.1016/j.seppur.2017.12.013 CrossRefGoogle Scholar
  9. Filly A, Fernandez X, Minuti M, Visinoni F, Cravotto G, Chemat F (2014) Solvent-free microwave extraction of essential oil from aromatic herbs: from laboratory to pilot and industrial scale. Food Chem 150:193–198.  https://doi.org/10.1016/j.foodchem.2013.10.139 CrossRefGoogle Scholar
  10. Gavahian M, Farahnaky A, Farhoosh R, Javidnia K, Shahidi F (2015) Extraction of essential oils from Mentha piperita using advanced techniques: microwave versus ohmic assisted hydrodistillation. Food Bioprod Process 94:50–58.  https://doi.org/10.1016/j.fbp.2015.01.003 CrossRefGoogle Scholar
  11. González-Rivera J, Duce C, Falconieri D, Ferrari C, Ghezzi L, Piras A, Tine MR (2016) Coaxial microwave assisted hydrodistillation of essential oils from five different herbs (lavender, rosemary, sage, fennel seeds and clove buds): chemical composition and thermal analysis. Innov Food Sci Emerg Technol 33:308–318.  https://doi.org/10.1016/j.ifset.2015.12.011 CrossRefGoogle Scholar
  12. Hashemimoghadam H, Feyzi P, Kamali H, Nematollahi A (2013) Comparison of microwave extraction and hydrodistillation of essential oil from Biebersteinia multifida DC. Conjunction with gas chromatography–mass spectroscopy: optimization via Box-Behnken. JNKUMS 4:13–21.  https://doi.org/10.29252/jnkums.4.5.S5.13 CrossRefGoogle Scholar
  13. Karabegović IT, Stojičević SS, Veličković DT, Nikolić NČ, Lazić ML (2013) Optimization of microwave-assisted extraction and characterization of phenolic compounds in cherry laurel (Prunus laurocerasus) leaves. Sep Purif Technol 120:429–436.  https://doi.org/10.1016/j.seppur.2013.10.021 CrossRefGoogle Scholar
  14. Karakaya S, El SN, Karagozlu N, Sahin S, Sumnu G, Bayramoglu B (2014) Microwave-assisted hydrodistillation of essential oil from rosemary. J Food Sci Technol 51:1056–1065.  https://doi.org/10.1007/s13197-011-0610-y CrossRefGoogle Scholar
  15. Kusuma HS, Mahfud M (2017) Comparison of conventional and microwave-assisted distillation of essential oil from Pogostemon cablin leaves: analysis and modelling of heat and mass transfer. JARMAP 4:55–65.  https://doi.org/10.1016/j.jarmap.2016.08.002 Google Scholar
  16. Li S et al (2016) Ionic liquid-mediated microwave-assisted simultaneous extraction and distillation of gallic acid, ellagic acid and essential oil from the leaves of Eucalyptus camaldulensis. Sep Purif Technol 168:8–18.  https://doi.org/10.1016/j.seppur.2016.05.013 CrossRefGoogle Scholar
  17. Lopresto CG, Petrillo F, Casazza AA, Aliakbarian B, Perego P, Calabrò V (2014) A non-conventional method to extract D-limonene from waste lemon peels and comparison with traditional Soxhlet extraction. Sep Purif Technol 137:13–20.  https://doi.org/10.1016/j.seppur.2014.09.015 CrossRefGoogle Scholar
  18. Mezza GN, Borgarello AV, Grosso NR, Fernandez H, Pramparo MC, Gayol MF (2018) Antioxidant activity of rosemary essential oil fractions obtained by molecular distillation and their effect on oxidative stability of sunflower oil. Food Chem 242:9–15.  https://doi.org/10.1016/j.foodchem.2017.09.042 CrossRefGoogle Scholar
  19. Qi X-L et al (2014) Solvent-free microwave extraction of essential oil from pigeon pea leaves [Cajanus cajan (L.) Millsp.] and evaluation of its antimicrobial activity. Ind Crops Prod 58:322–328.  https://doi.org/10.1016/j.indcrop.2014.04.038 CrossRefGoogle Scholar
  20. Ranjbar Nedamani E, Sadeghi Mahoonak A, Ghorbani M, Kashaninejad M (2015) Evaluation of antioxidant interactions in combined extracts of green tea (Camellia sinensis), rosemary (Rosmarinus officinalis) and oak fruit (Quercus branti). J Food Sci Technol 52:4565–4571.  https://doi.org/10.1007/s13197-014-1497-1 CrossRefGoogle Scholar
  21. Zermane A, Larkeche O, Meniai A-H, Crampon C, Badens E (2016) Optimization of Algerian rosemary essential oil extraction yield by supercritical CO2 using response surface methodology. C R Chim 19:538–543.  https://doi.org/10.1016/j.crci.2015.08.011 CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2018

Authors and Affiliations

  • Maryam Akhbari
    • 1
  • Saeed Masoum
    • 2
  • Fahimeh Aghababaei
    • 1
  • Sepideh Hamedi
    • 3
  1. 1.Essential Oils Research InstituteUniversity of KashanKashanIran
  2. 2.Department of Analytical Chemistry, Faculty of ChemistryUniversity of KashanKashanIran
  3. 3.Bio-refinery Group, New Technologies Engineering FacultyShahid Beheshti UniversityZirabIran

Personalised recommendations