Journal of Food Science and Technology

, Volume 54, Issue 13, pp 4501–4509 | Cite as

Optimization and characterization of high pressure homogenization produced chemically modified starch nanoparticles

  • Yongbo Ding
  • Jianquan KanEmail author
Original Article


Chemically modified starch (RS4) nanoparticles were synthesized through homogenization and water-in-oil mini-emulsion cross-linking. Homogenization was optimized with regard to z-average diameter by using a three-factor-three-level Box–Behnken design. Homogenization pressure (X1), oil/water ratio (X2), and surfactant (X3) were selected as independent variables, whereas z-average diameter was considered as a dependent variable. The following optimum preparation conditions were obtained to achieve the minimum average size of these nanoparticles: 50 MPa homogenization pressure, 10:1 oil/water ratio, and 2 g surfactant amount, when the predicted z-average diameter was 303.6 nm. The physicochemical properties of these nanoparticles were also determined. Dynamic light scattering experiments revealed that RS4 nanoparticles measuring a PdI of 0.380 and an average size of approximately 300 nm, which was very close to the predicted z-average diameter (303.6 nm). The absolute value of zeta potential of RS4 nanoparticles (39.7 mV) was higher than RS4 (32.4 mV), with strengthened swelling power. X-ray diffraction results revealed that homogenization induced a disruption in crystalline structure of RS4 nanoparticles led to amorphous or low-crystallinity. Results of stability analysis showed that RS4 nanosuspensions (particle size) had good stability at 30 °C over 24 h.


Homogenization Mini-emulsion cross-linking Box–Behnken design RS4 nanoparticle Physicochemical properties 



This work was supported by the Fundamental Research Funds for the Central Universities under Grants No. XDJK2017D124.


  1. Antonietti M, Landfester K (2002) Polyreactions in miniemulsions. Prog Polym Sci 27:689–757CrossRefGoogle Scholar
  2. Asua JM (2002) Miniemulsion polymerization. Prog Polym Sci 27:1283–1346CrossRefGoogle Scholar
  3. Chen W, Wang WP, Zhang HS, Huang Q (2012) Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletus edulis mycelia using response surface methodology. Carbohyd Polym 87:614–619CrossRefGoogle Scholar
  4. Chivrac F, Pollet E, Avérous L (2009) Progress in nano-biocomposites based on polysaccharides and nanoclays. Mater Sci Eng R 67:1–17CrossRefGoogle Scholar
  5. Ding Y, Kan J (2016) Characterization of nanoscale retrograded starch prepared by a sonochemical method. Starch-Starke 68:264–273CrossRefGoogle Scholar
  6. Ding Y, Zheng J, Xia X, Ren T, Kan J (2016a) Box–behnken design for the optimization of nanoscale retrograded starch formation by high-power ultrasonication. Lwt-Food Sci Technol 67:206–213CrossRefGoogle Scholar
  7. Ding Y, Zheng J, Xia X, Ren T, Kan J (2016b) Preparation and characterization of resistant starch type IV nanoparticles through ultrasonication and miniemulsion cross-linking. Carbohyd Polym 141:151–159CrossRefGoogle Scholar
  8. Ding Y, Zheng J, Zhang F, Kan J (2016c) Synthesis and characterization of retrograded starch nanoparticles through homogenization and miniemulsion cross-linking. Carbohyd Polym 151:656–665CrossRefGoogle Scholar
  9. Espadabellido E, Ferreirogonzález M, Carrera C, Palma M, Barroso CG, Barbero GF (2016) Optimization of the ultrasound-assisted extraction of anthocyanins and total phenolic compounds in mulberry (Morus nigra) pulp. Food Chem 219:23–32CrossRefGoogle Scholar
  10. Fuentes-Zaragoza E, Sánchez-Zapata E, Sendra E, Sayas E, Navarro C, Fernández-López J, Pérez-Alvarez JA (2011) Resistant starch as prebiotic: a review. Starch-Starke 63:406–415CrossRefGoogle Scholar
  11. Hernandez M, Recio G, Martin-Palma RJ, Garcia-Ramos JV, Domingo C, Sevilla P (2012) Surface enhanced fluorescence of anti-tumoral drug emodin adsorbed on silver nanoparticles and loaded on porous silicon. Nanoscale Res Lett 7:364CrossRefGoogle Scholar
  12. Lamprecht A, Ubrich N, Pérez MH, Lehr CM, Hoffman M, Maincent P (2000) Influences of process parameters on nanoparticle preparation performed by a double emulsion pressure homogenization technique. Int J Pharm 196:177–182CrossRefGoogle Scholar
  13. Le Corre D, Bras J, Dufresne A (2010) Starch nanoparticles: a review. Biomacromolecules 11:1139–1153CrossRefGoogle Scholar
  14. Liu R, Ma G, Meng FT, Su ZG (2005) Preparation of uniform-sized PLA microcapsules by combining Shirasu porous glass membrane emulsification technique and multiple emulsion-solvent evaporation method. J Control Release 103:31–43CrossRefGoogle Scholar
  15. Mahmoudi Najafi SH, Baghaie M, Ashori A (2016) Preparation and characterization of acetylated starch nanoparticles as drug carrier: ciprofloxacin as a model. Int J Biol Macromol 87:48–54CrossRefGoogle Scholar
  16. Niemann B, Sundmacher K (2010) Nanoparticle precipitation in microemulsions: population balance model and identification of bivariate droplet exchange kernel. J Colloid Interface Sci 342:361–371CrossRefGoogle Scholar
  17. Pang SC, Chin SF, Tay SH, Tchong FM (2011) Starch–maleate–polyvinyl alcohol hydrogels with controllable swelling behaviors. Carbohyd Polym 84:424–429CrossRefGoogle Scholar
  18. Patel CM, Chakraborty M, Murthy ZVP (2016) Fast and scalable preparation of starch nanoparticles by stirred media milling. Adv Powder Technol 27:1287–1294CrossRefGoogle Scholar
  19. Prabhu S, Vaideki K, Anitha S (2017) Effect of microwave argon plasma on the glycosidic and hydrogen bonding system of cotton cellulose. Carbohyd Polym 156:34–44CrossRefGoogle Scholar
  20. Shi AM, Li D, Wang LJ, Li BZ, Adhikari B (2011) Preparation of starch-based nanoparticles through high-pressure homogenization and miniemulsion cross-linking: influence of various process parameters on particle size and stability. Carbohyd Polym 83:1604–1610CrossRefGoogle Scholar
  21. Swamy GJ, Muthukumarappan K (2016) Optimization of continuous and intermittent microwave extraction of pectin from banana peels. Food Chem 220:108–114CrossRefGoogle Scholar
  22. Thys RC, Westfahl JH, Noreña CP, Marczak LD, Silveira NP, Cardoso MB (2008) Effect of the alkaline treatment on the ultrastructure of C-type starch granules. Biomacromolecules 9:1894–1901CrossRefGoogle Scholar
  23. Wang F, Deng R, Wang J, Wang Q, Han Y, Zhu H, Liu X (2011) Tuning upconversion through energy migration in core–shell nanoparticles. Nat Mater 10:968–973CrossRefGoogle Scholar
  24. Xu J, Sun J, Wang Y, Sheng J, Wang F, Sun M (2014) Application of iron magnetic nanoparticles in protein immobilization. Molecules 19:11465–11486CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  1. 1.College of Food ScienceSouthwest UniversityChongqingPeople’s Republic of China
  2. 2.Laboratory of Quality and Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing)Ministry of AgricultureChongqingPeople’s Republic of China

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