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Journal of Food Science and Technology

, Volume 54, Issue 13, pp 4416–4426 | Cite as

Differential accumulation of β-carotene and tissue specific expression of phytoene synthase (MaPsy) gene in banana (Musa sp) cultivars

  • R. DhandapaniEmail author
  • V. P. Singh
  • A. Arora
  • R. C. Bhattacharya
  • Ambika Rajendran
Original Article

Abstract

An experiment was conducted with twelve major Indian banana cultivars to investigate the molecular relationship between the differential accumulation of β-carotene in peel and pulp of the banana fruit and carotenoid biosynthetic pathway genes. The high performance liquid chromatography showed that all banana cultivars accumulated two–three fold more β-carotene in non-edible portion of the banana fruit. However, Nendran, a famous orange fleshed cultivar of South India, had high β-carotene content (1362 µg/100 g) in edible pulp. The gene encoding Musa accuminata phytoene synthase (MaPsy) was successfully amplified using a pair of degenerate primers designed from Oncidium orchid. The deduced amino acid sequences shared a high level of identity to phytoene synthase gene from other plants. Gene expression analysis confirmed the presence of two isoforms (MaPsy1 and MaPsy2) of MaPsy gene in banana fruits. Presence of two isoforms of MaPsy gene in peel and one in pulp confirmed the differential accumulation of β-carotene in banana fruits. However, Nendran accumulated more β-carotene in edible pulp due to presence of both the isoforms of MaPsy gene. Thus, carotenoid accumulation is a tissue specific process strongly dependent on differential expression pattern of two isoforms of MaPsy gene in banana.

Keywords

Musa accuminata Nendran Vitamin A Carotenoids β-Carotene Phytoene synthase 

Supplementary material

13197_2017_2918_MOESM1_ESM.doc (4.7 mb)
Supplementary material 1 (DOC 4825 kb)

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Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  • R. Dhandapani
    • 1
    Email author
  • V. P. Singh
    • 1
  • A. Arora
    • 1
  • R. C. Bhattacharya
    • 2
  • Ambika Rajendran
    • 3
  1. 1.Division of Plant PhysiologyICAR-Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.ICAR-National Research Centre for Plant BiotechnologyNew DelhiIndia
  3. 3.Division of GeneticsICAR-Indian Agricultural Research InstituteNew DelhiIndia

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