Abstract
Pectic oligosaccharides (POS) have been indicated as novel candidate prebiotics. Traditionally, POS are produced from pectin-rich by-products using a two-step process involving extraction of the pectin, followed by its hydrolysis into POS. A one-step approach, in which the POS is directly produced from the raw material, might provide a more efficient alternative. Thus, the main aim of this paper was to investigate a one-step enzymatic hydrolysis approach to directly produce POS from sugar beet pulp (SBP). The POS yield was investigated as a function of the process parameters, as well as raw material characteristics. A statistically-based response surface methodology, using a central composite design was applied, to investigate the individual as well as the combined influences of the diverse parameters. The model was confirmed by a validation experiment, carried out at 135 g/l substrate concentration, 0.75 FPU/g SBP enzyme concentration, 0.8 mm particle size and 3 h hydrolysis time. Under these conditions, a POS-rich hydrolysate was obtained, containing rhamnose, arabinose, galactose, xylose and galacturonic acid, at 0.9, 15.2, 5.1, 1.4, and 13.2 g/l, respectively, enzymes were added each at 20 FPU/g dry matter (DM).
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Acknowledgement
The authors acknowledge the work supported by the European commission (FP7, NOSHAN, contract no. 312140). The authors also acknowledge IGV for providing the raw material. Neha Babbar gratefully acknowledges the PhD scholarship grant from VITO and University of Parma, Italy.
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Babbar, N., Dejonghe, W., Sforza, S. et al. Enzymatic pectic oligosaccharides (POS) production from sugar beet pulp using response surface methodology. J Food Sci Technol 54, 3707–3715 (2017). https://doi.org/10.1007/s13197-017-2835-x
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DOI: https://doi.org/10.1007/s13197-017-2835-x