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Inhibition of Shewanella spp. growth by Syzygium australe and Syzygium luehmannii extracts: natural methods for the prevention of fish spoilage

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Abstract

Syzygium australe and Syzygium luehmannii fruit and leaf were investigated for their ability to inhibit Shewanella spp. growth. Extracts of both Syzygium spp. displayed potent growth inhibitory properties against all Shewanella spp. tested in disc diffusion and liquid diffusion assays. In general, S. australe extracts were more potent inhibitors of Shewanella spp. growth, and the fruit extracts were generally better than the corresponding leaf extracts. The methanolic S. australe fruit extract was a particularly potent inhibitor of all Shewanella spp. growth, with MIC values as low as 87 µg/mL. The aqueous and ethyl acetate S. australe fruit extracts were similarly potent inhibitors of Shewanella spp. growth, albeit with slightly higher MIC values. Several other Syzygium spp. extracts also were potent bacterial growth inhibitors, albeit with MIC values generally >1000 µg/mL. The most potent S. australe fruit extracts were nontoxic in the Artemia franciscana bioassay, with LC50 values substantially >1000 µg/mL. The potent bacterial growth inhibitory activity and lack of toxicity of the S. australe fruit extracts indicate their potential as natural fish and seafood preservatives.

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Acknowledgements

The authors are most grateful to Professor Kenneth Nealson of the University of Southern California, USA for the gift of all environmental Shewanella spp. strain used in this study. Financial support for this work was provided by the Environmental Futures Research Institute and the School of Natural Sciences, Griffith University, Australia.

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Authors and Affiliations

  1. Environmental Futures Research Institute, Nathan Campus, Griffith University, Brisbane, Australia

    Shweta Murhekar, Jeremy Colin Brownlie & Ian Edwin Cock

  2. School of Natural Sciences, Nathan Campus, Griffith University, Brisbane, Australia

    Shweta Murhekar, Mitchell Henry Wright, Anthony Carlson Greene, Jeremy Colin Brownlie & Ian Edwin Cock

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  1. Shweta Murhekar
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  2. Mitchell Henry Wright
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  3. Anthony Carlson Greene
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  4. Jeremy Colin Brownlie
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  5. Ian Edwin Cock
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Correspondence to Ian Edwin Cock.

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Murhekar, S., Wright, M.H., Greene, A.C. et al. Inhibition of Shewanella spp. growth by Syzygium australe and Syzygium luehmannii extracts: natural methods for the prevention of fish spoilage. J Food Sci Technol 54, 3314–3326 (2017). https://doi.org/10.1007/s13197-017-2782-6

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  • DOI: https://doi.org/10.1007/s13197-017-2782-6

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