Abstract
Considering the significance of natural antioxidants to preserve meat, the present study was undertaken to evaluate the efficacy of a deflavored and decolorised extract of rosemary (StabilRose™) for the production and preservation of naturally colored fresh meat. Oxidative rancidity of meat and color degradation of paprika oleoresin were exploited as model systems and compared with butylated hydroxyanisole (BHA). The results showed similar efficacy for 3% carnosic acid extract and BHA, with further enhancement in efficacy with respect to the carnosic acid content. A synergetic antioxidant effect of carnosol on carnosic acid content was also noticed to an extent of 1:1 (w/w) ratio, and further increase in carnosol content showed no improvement in the antioxidant efficacy. Finally, stabilized paprika and optimized rosemary extract containing carnosic acid and carnosol in 1:1 (w/w) ratio was successfully applied to produce naturally colored meat suitable for storage at 4 ± 1 °C.






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References
Aguilar F, Autrup H, Barlow S et al (2008) Use of rosemary extracts as a food additive-scientific opinion of the panel on food additives, flavourings, processing aids and materials in contact with food. EFSA J 721:1–29
Basappa MN, Subbaiah V, Muthupalani M et al (2014) Antioxidant activity of carnosic acid and rosmarinic acid in raw and cooked ground chicken patties. J Sci Food Agr 94:273–279
Bozin B, Mimica-Dukic N, Samojlik I, Jovin E (2007) Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J Agric Food Chem 55:7879–7885
Brand-Williams W, Cuvelier M-E, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28:25–30
Breivik H, Jacobsenq C, Moen V, Vojnovic T (2009) Antioxidant composition for marine oils comprising tocopherol, rosemary extract, ascorbic acid and green tea extract. Patent WO 2010033034 A1
Dave D, Ghaly AE (2011) Meat spoilage mechanisms and preservation techniques: a critical review. Am J Agric Biol Sci 6:486–510
Decker E, Mei L (1996) Antioxidant mechanisms and applications in muscle foods. Proc Recipr Meat Conf 49:64–72
Erkan N, Ayranci G, Ayranci E (2008) Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 110:76–82
European Food Safety Authority (EFSA)–Panel on Food Additives and Nutrient Sources added to Food (2011) Scientific opinion on the reevaluation of butylated hydroxytoluene BHT (E 321) as a food additive. EFSAJ 9:2392
European Food Safety Authority (EFSA)–Panel on Food Additives and Nutrient Sources added to Food (2015) Extension of use of extracts of rosemary (E 392) in fat-based spreads. EFSAJ 13:4090
Fasseas MK, Mountzouris KC, Tarantilis PA, Polissiou M, Zervas G (2008) Antioxidant activity in meat treated with oregano and sage essential oils. Food Chem 106:1188–1194
Faustman C, Sun Q, Mancini R, Suman SP (2010) Myoglobin and lipid oxidation interactions: mechanistic bases and control. Meat Sci 86:86–94
Gray J, Gomaa E, Buckley D (1996) Oxidative quality and shelf life of meats. Meat Sci 43:111–123
Kilic B, Simsek A, Claus J, Atilgan E (2014) Encapsulated phosphates reduce lipid oxidation in both ground chicken and ground beef during raw and cooked meat storage with some influence on color, pH, and cooking loss. Meat Sci 97:93–103
Maqsood S, Benjakul S (2011) Comparative studies on molecular changes and pro-oxidative activity of haemoglobin from different fish species as influenced by pH. Food Chem 124:875–883
Ortega-Ramirez LA, Rodriguez-Garcia I, Leyva JM et al (2014) Potential of medicinal plants as antimicrobial and antioxidant agents in food industry: a hypothesis. J Food Sci 79:R129–R137
Park Y, Kelleher SD, McClements DJ, Decker EA (2004) Incorporation and stabilization of omega-3 fatty acids in surimi made from cod, Gadus morhua. J Agric Food Chem 52:597–601
Regnier T, Combrinck S, Du-Plooy W (2012) Essential oils and other plant extracts as food preservatives. In: Bhat R, Karim A, Paliyath G (eds) Progress in food preservation. Wiley-Blackwell, Oxford, pp 539–579
Rohlik B-A, Pipek P, Panek J (2013) The effect of natural antioxidants on the colour and lipid stability of paprika salami. Czech J Food Sci 31:307–312
Rojas M, Brewer M (2007) Effect of natural antioxidants on oxidative stability of cooked, refrigerated beef and pork. J Food Sci 72:S282–S288
Sandeep S, Souravh B (2014) Rosemary, (Rosmarinus Officinalis) Linn. An updated review. AAJST 1:069–077
Serrano R, Ortuño J, Bañón S (2014) Improving the sensory and oxidative stability of cooked and chill-stored lamb using dietary rosemary diterpenes. J Food Sci 79:S1805–S1810
Shahidi F (2000) Antioxidants in food and food antioxidants. Food/Nahrung 44:158–163
Shantha NC, Decker EA (1994) Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. J AOAC Int 77:421–424
Singh A, Sharma PK, Garg G (2010) Natural products as preservatives. Int J Pharma Biosci 1(P601):P612
Suh H-J, Chung M-S, Cho Y-H et al (2005) Estimated daily intakes of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ) antioxidants in Korea. Food Addit Contam 22:1176–1188
Thorsen MA, Hildebrandt KS (2003) Quantitative determination of phenolic diterpenes in rosemary extracts: aspects of accurate quantification. J Chromatogr A 995:119–125
Todd Jr PH (1991) Color-stabilized paprika pigment compositions and foods colored therewith having increased resistance to oxidative color fading. US Patent 5059437
Union European (2008) Regulation (EC) No 1334/2008 of the European Parliament and of the Council of 16 December 2008 on flavourings and certain food ingredients with flavouring properties for use in and on foods and amending Council Regulation (EEC) No 1601/91, Regulations (EC) No 2232/96 and (EC) No 110/2008 and Directive 2000/13/EC. Off J EU 51:34–50
US Food and Drug Administration, USFDA (2013) CFR—code of federal regulations title 21. USFDA, Silver Spring
Véronique COMA (2008) Bioactive packaging technologies for extended shelf life of meat-based products. Meat Sci 78:90–103
Wattenberg LW (2014). Report on carcinogens, butylated hydroxyanisole. National Toxicology Program, Department of Health and Human Services, 13th ed (CAS No. 25013-16-5)
Williams G, Iatropoulos M, Whysner J (1999) Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food Chem Toxicol 37:1027–1038
Witte VC, Krause GF, Bailey ME (1970) A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. J Food Sci 35:582–585
Wojdyło A, Oszmiański J, Czemerys R (2007) Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 105:940–949
Yi J, Dong W, Zhu Z et al (2015) Surfactant concentration, antioxidants, and chelators influencing oxidative stability of water-in-walnut oil emulsions. J Am Oil Chem Soc 92:1093–1102
Acknowledgements
The authors are grateful to M/s Akay Flavours & Aromatics Pvt. Ltd, Cochin, India for financial support and for the lot samples produced in their GMP-certified manufacturing plant.
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The authors have declared that there is no conflict of interest. StabilRose™ is the registered trademark of M/s Akay Flavours & Aromatics Pvt. Ltd, for their rosemary antioxidants.
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Rajeev, P.S., Johannah, N.M., Gopakumar, G. et al. Optimization of antioxidant efficacy of a deflavored and decolorized rosemary extract: effect of carnosol content on the oxidative stability of paprika colored beef patties. J Food Sci Technol 54, 1665–1677 (2017). https://doi.org/10.1007/s13197-017-2599-3
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DOI: https://doi.org/10.1007/s13197-017-2599-3


