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Collagen extraction from mussel byssus: a new marine collagen source with physicochemical properties of industrial interest

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Abstract

Mussel byssus is a by-product of mussel production and is a potential source of collagen. The goal of this study was to extract collagen from the byssus of Chilean mussel using an enzymatic method and characterize it. A pepsin-aided extraction method was employed where first an enzymatic hydrolysis at two pepsin/substrate ratios (1:50 or 4:50) and times (4 or 24 h) was done. Extraction was conducted at 80 °C for 24 h, in a 0.5 N acetic acid solution. All samples were analyzed for collagen content, amino acid profile, turbidity, viscosity, solubility, denaturation temperature and surface tension. Hydrolysis time had significant effect on collagen content, hydroxyproline content and extraction yield. Hydrolysis with a pepsin/byssus ratio of 4:50 for 24 h gave the better extraction performance with values of 69 mg/g protein, 1.8 mg/g protein and 30%, for collagen content, hydroxyproline content and extraction yield, respectively. No differences were found for the viscosity and surface tension of collagen dispersions, suggesting that the enzymatic hydrolysis did not affect the integrity of the collagen molecule. Denaturation temperature of freeze-dried byssus collagen presented a high value (83–91 °C), making this kind of collagen a very interesting material for encapsulation of bioactive molecules and for biomedical applications.

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Acknowledgements

R. N. Zúñiga thanks CONICYT for financial support by means of the FONDECYT Project 11110156. The assistance of Dr. Oscar Marambio in DSC analyses is gratefully appreciated.

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Authors and Affiliations

  1. Department of Biotechnology, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago, Chile

    F. Rodríguez, G. González & R. N. Zúñiga

  2. Department of Chemical and Biochemical Engineering, Instituto Tecnológico de Zacatepec, Calzada Tecnológico 27, Zacatepec, Morelos, Mexico

    L. Morán

  3. Department of Chemistry, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago, Chile

    E. Troncoso

  4. Programa Institucional de Fomento a la Investigación, Desarrollo e Innovación, Universidad Tecnológica Metropolitana, Ignacio Valdivieso 2409, San Joaquín, Santiago, Chile

    E. Troncoso

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  1. F. Rodríguez
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Correspondence to R. N. Zúñiga.

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Rodríguez, F., Morán, L., González, G. et al. Collagen extraction from mussel byssus: a new marine collagen source with physicochemical properties of industrial interest. J Food Sci Technol 54, 1228–1238 (2017). https://doi.org/10.1007/s13197-017-2566-z

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  • DOI: https://doi.org/10.1007/s13197-017-2566-z

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