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Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts

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Abstract

The content of phenolic compounds (TPC) and glucans, as well as the effectiveness of antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts were determined and compared. The content of glucans (total, α- and β-) strongly depended on the extraction time and particle size, but only interaction of these parameters influenced the TPC. Gallic acid, quercetin, trans-cinnamic acid, kaempferol, hesperetin and naringenin were detected in extracts by HPLC–DAD. The most abundant phenols were hesperetin (1.875–3.222 µg/g) and naringenin (1.235–2.856 µg/g). The ethanol extracts exhibited noteworthy antioxidant activity, but the significant amount of phenolic compounds was strongly linked to polysaccharides, and hence reduced their antioxidant capacity. The results of the antiproliferative activity in vitro showed that the analyzed extracts were the most effective against HeLa cells. Significant correlations were observed between the antiproliferative effect and the TPC/glucan content of extracts.

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References

  • Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  • Cao QZ, Lin ZB (2006) Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell. Life Sci 78:1457–1463

    Article  CAS  Google Scholar 

  • Chen NH, Liu JW, Zhong JJ (2010) Ganoderic acid T inhibits tumor invasion in vitro and in vivo through inhibition of MMP expression. Pharmacol Rep 62:157–170

    Article  Google Scholar 

  • Ćilardžić J, Vukojević J, Stajić M, Stanojković T, Glamočlija J (2014) Biological activity of Ganoderma lucidum basidiocarps cultivated on alternative and commercial substrate. J Ethnopharmacol 155:312–319

    Article  Google Scholar 

  • Ferreira ICFR, Barros L, Abreu RMV (2009) Antioxidant in wild mushrooms. Curr Med Chem 16:1543–1560

    Article  CAS  Google Scholar 

  • Ferreira ICFR, Heleno SA, Reis FS, Stojković D, Queiroz MJRP, Vasconcelos HM, Sokolović M (2015) Chemical features of Ganoderma polysaccharide with antioxidant, antitumor and antimicrobial activities. Phytochemistry 114:38–55

    Article  CAS  Google Scholar 

  • Galor SW, Yuen J, Buswell AJ, Benzie FF (2011) Ganoderma lucidum (Lingzhi, Reshi) A medicinal mushroom. In: Galor SW, Benzie FF (eds) Herbal medicine, bimolecular and clinical aspects, 2nd edn. CRC Press Taylor & Francis Group, Boca Ralon, pp 175–200

    Chapter  Google Scholar 

  • Heleno SA, Barros L, Martins A, Queiroz MJRP, Santos-Buelga C, Ferreira ICFR (2012) Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: a comparative study of the antioxidant potential of phenolic and polysaccharides extracts. Food Res Int 46:135–140

    Article  CAS  Google Scholar 

  • Kaneda H, Kobayashi N, Furusho S, Sahara H, Koshino S (1995) Reducing activity and flavor stability of beer. Master Brew Associ Am Tech Q 32:90–94

    CAS  Google Scholar 

  • Karaman M, Jovin E, Malbaša R, Matavuly M, Popović M (2010) Medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents. Phytother Res 24:1473–1481

    Article  CAS  Google Scholar 

  • Kim MY, Seguin P, Ahn JK et al (2008) Phenolic compounds concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem 56:7265–7270

    Article  CAS  Google Scholar 

  • Kozarski M, Klaus A, Nikšić M, Jakovljević D, Helsper JPFC, Griensven LJL (2011) Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganodrema lucidum and Pleurotus linteu. Food Chem 129:1667–1675

    Article  CAS  Google Scholar 

  • Kozarski M, Klaus A, Nikšić M et al (2012) Antioxidative activities and chemical characterization of polysaccharide extracts from widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. J Food Compos Anal 26:144–153

    Article  CAS  Google Scholar 

  • Li N, Hu YL, He CX, Hu CJ, Zhou J, Tang GP, Gao JQ (2010) Preparation, characterisation and anti-tumour activity of Ganoderma lucidum polysaccharide nanoparticles. J Pharm Pharmacol 62(1):139–144

    Article  CAS  Google Scholar 

  • Liu X, Yuan JP, Chung CK, Chen XJ (2002) Antitumor activity of sporoderm-broken germinating spores of Ganoderma lucidum. Cancer Lett 182:155–161

    Article  CAS  Google Scholar 

  • Liu RM, Li YB, Liang XF, Liu HZ, Xiao JH, Zhong JJ (2015) Structurally related ganoderic acids induce apoptosis in human cervical cancer HeLa cells: involvement of oxidative stress and antioxidant protective system. Chem Biol Interact 240:134–144

    Article  CAS  Google Scholar 

  • Luthria DL (2008) Influence of experimental conditions on the extraction of phenolic compounds from parsley (Petroselinum crispum) flakes using a pressurized liquid extractor. Food Chem 107:745–752

    Article  CAS  Google Scholar 

  • Pecić S, Nikićević N, Veljović M, Jadranin M, Tešević V, Belović M, Nikšić M (2016) The influence of extraction parameters on physicochemical properties of special grain brandies with Ganoderma lucidum. Chem Ind Chem Eng Q 22(2):181–189

    Article  Google Scholar 

  • Rakić G, Grgurić-Šipka S, Kaluđerović G, Bette M, Filipović L, Aranđelović S, Radulović S, Tešić Z (2012) Synthesis, spectroscopic, X-ray characterization and in vitro cytotoxic testing results of activity of five new trans-platinum(IV) complexes with functionalized pyridines. Eur J Med Chem 55:214–219

    Article  Google Scholar 

  • Rawat A, Mohsin M, Negi PS, Sah AN, Singh S (2013) Evalution of polyphenolic contents and antioxidant activity of wildly collected Ganoderma lucidum from central Himalayan hills of India. Asian J Plant Sci Res 3(3):85–90

    CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C (1999) Antioxidant activity applying an improved ABTS radical cationde colorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  • Rice-Evans CA, Miller JM, Paganga G (1996) Glucosinolates and phenolics as antioxidants from structure-antioxidant activity relationship of flavon-plant foods. Free Radic Biol Med 20:933–956

    Article  CAS  Google Scholar 

  • Saltarelli R, Ceccaroli P, Iotti M, Zambonelli A, Buffalini M, Casadei L, Vallorani L, Stocchi V (2009) Biochemical characterisation and antioxidant activity of mycelium of Ganoderma lucidum from Central Italy. Food Chem 116(1):143–151

    Article  CAS  Google Scholar 

  • Saltarelli R, Ceccaroli P, Buffalini M, Vallorani L, Casadei L, Zambonelli A, Iotti M, Badalyan S, Stocchi V (2015) Biochemical characterization and antioxidant and antiproliferative activities of different Ganoderma collections. J Mol Microbiol Biotechnol 25(1):16–25

    Article  CAS  Google Scholar 

  • Sheikh IA, Vyas D, Ganaie MA, Dehariya K, Singh V (2014) HPLC determination of phenolics and free radical scavenging activity of ethanolic extracts of two polypore mushrooms. Int J Pharm Pharm Sci 6:679–684

    CAS  Google Scholar 

  • Silva D (2003) Ganoderma lucidum (Reishi) in cancer treatment. Integr Cancer Ther 2:358–364

    Article  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphor molybdic-phospho tungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Stojković DS, Barros L, Calhelha RC et al (2014) A detailed comparative study between chemical and bioactive properties of Ganoderma lucidum from different origins. Int J Food Sci Nutr 65:42–47

    Article  Google Scholar 

  • Su CH, Lai MN, Lin CC, Ng LT (2016) Comparative characterization of physicochemical properties and bioactivities of polysaccharides from selected medicinal mushrooms. Appl Microbiol Biotechnol 100:4385–4393

    Article  CAS  Google Scholar 

  • Supino R (1995) MTT assays. In: O’Hare S, Atterwill CK (eds) Vitro toxicity testing protocols. Humana Press, New York, pp 137–149

    Chapter  Google Scholar 

  • Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60:258–274

    Article  CAS  Google Scholar 

  • Wasser SP (2005) Reishi or Ling Zhi (Ganoderma lucidum). In: Coates PM, Betz JM, Blackman MR, Grass GM, Levine M, Moss J, White J (eds) Encyclopedia of dietary supplements. Marcel Dekker, New York, pp 603–622

    Google Scholar 

  • Wasser SP (2010) Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms 12:1–16

    Article  CAS  Google Scholar 

  • Yuen JW, Gohel MD (2005) Anticancer effects of Ganoderma lucidum: a review of scientific evidence. Nutr Cancer 53:11–17

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was performed within the framework of the research Projects Nos. III 46001 and III 41026 supported by the Ministry of Education, Sciences and Technological Development, Republic of Serbia.

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Authors and Affiliations

  1. Department for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia

    Sonja Veljović, Mile Veljović, Ninoslav Nikićević, Saša Despotović & Miomir Nikšić

  2. Economics Institute, University of Belgrade, KraljaMilana 16, 11000, Belgrade, Serbia

    Sonja Veljović

  3. Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia

    Siniša Radulović & Lana Filipović

Authors
  1. Sonja Veljović
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  2. Mile Veljović
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  5. Siniša Radulović
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Correspondence to Sonja Veljović.

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Veljović, S., Veljović, M., Nikićević, N. et al. Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts. J Food Sci Technol 54, 1312–1320 (2017). https://doi.org/10.1007/s13197-017-2559-y

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  • DOI: https://doi.org/10.1007/s13197-017-2559-y

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