Journal of Food Science and Technology

, Volume 54, Issue 3, pp 792–801 | Cite as

Isolation, molecular characterization and screening of indigenous lactobacilli for their abilities to produce bioactive conjugated linoleic acid (CLA)

Original Article

Abstract

Ingestion of conjugated linoleic acid poised many health benefits; however, amount of CLA one can get through generalized diet in is inadequate in exerting the desired benefits. Therefore, presence of CLA producing lactobacilli in dairy fermented foods has a tremendous potential to increase the CLA content. Therefore, present study was focused to isolate and characterize CLA producing lactobacilli from different dairy products and human faeces. Arguably, 283 lactobacilli were isolated from various sources and tested for CLA production. Fifty-seven CLA producing (≥20 µg/ml) lactobacilli were selected from screening in de Man, Rogosa and Sharpe (MRS) broth and reconstituted with skim milk (SM), supplemented with 0.5 mg/ml of linoleic acid. Positive strains were classified into—L. plantarum (44%), L. gasseri (30%), L. fermentum (21%) and L. salivarius (5%) species. Nineteen most efficient strains (CLA ≥25 µg/ml) were further assessed in SM for CLA production. Total 08 strains produced significantly higher CLA in SM than MRS and also produced cis 9, trans 11, trans 10, cis 12 and trans 9, trans 11 isomers. Overall, L. plantarum HIF15 was reported as the best producer of CLA and other 08 lactobacilli may be utilized for the formulation of CLA-enriched functional foods to support these bacteria to synthesize CLA in the human gut.

Keywords

Linoleic acid Biohydrogenation L. plantarum ct11 t10 c12 tt11 

Notes

Acknowledgements

We gratefully acknowledge ICAR-NDRI, Karnal for the financial support in form of fellowship to DKD. We express our sincere thanks to Dr. Ajai Kumar, AIRF (JNU), New Delhi for providing technical assistance for GC facility.

References

  1. Ando A, Ogawa J, Kishino S, Shimizu S (2003) CLA production from ricinoleic acid by lactic acid bacteria. J Am Oil Chem Soc 80:889–894CrossRefGoogle Scholar
  2. Andrade JC, Ascencao K, Gullon P, Henriques S, Pinto J, Rocha-Santos TA, Freitas AC, Gomes A (2012) Production of conjugated linoleic acid by food-grade bacteria: a review. Int J Dairy Technol 65:467–481CrossRefGoogle Scholar
  3. Barrett E, Ross R, Fitzgerald G, Stanton C (2007) Rapid screening method for analyzing the conjugated linoleic acid production capabilities of bacterial cultures. Appl Environ Microbiol 73:2333–2337CrossRefGoogle Scholar
  4. Chung SH, Kim IH, Park HG, Kang HS, Yoon CS, Jeong HY, Choi NJ, Kwon EG, Kim YJ (2008) Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017: utilization as a functional starter culture for milkfermentation. J Agric Food Chem 56:3311–3316CrossRefGoogle Scholar
  5. Dahiya DK, Puniya AK (2015) Evaluation of survival, free radical scavenging and human enterocyte adherence potential of lactobacilli with anti-obesity and anti-inflammatory CLA isomer-producing attributes. J Food Process Preserv 39:2866–2877CrossRefGoogle Scholar
  6. Dubernet S, Desmasures N, Guéguen M (2002) A PCR-based method for identification of lactobacilli at the genus level. FEMS Microbiol Lett 214:271–275CrossRefGoogle Scholar
  7. Farmani J, Safari M, Roohvand F, Razavi SH, Aghasadeghi MR, Noorbazargan H (2010) Conjugated linoleic acid-producing enzymes: a bioinformatics study. Eur J Lipid Sci Technol 112:1088–1100CrossRefGoogle Scholar
  8. Gorissen L, Leroy F, De Vuyst L, De Smet S, Raes K (2013) Bacterial production of conjugated linoleic and linolenic acid in foods: a technological challenge. Crit Rev Food Sci Nutr 55:1561–1574CrossRefGoogle Scholar
  9. Hennessy A, Ross R, Devery R, Stanton C (2009) Optimization of a reconstituted skim milk based medium for enhanced CLA production by Bifidobacteria. J Appl Microbiol 106:1315–1327CrossRefGoogle Scholar
  10. Hennessy AA, Barrett E, Ross RP, Fitzgerald GF, Devery R, Stanton C (2012) The production of conjugated α-linolenic, γ-linolenic and stearidonic acids by strains of bifidobacteria and propionibacteria. Lipids 47:313–327CrossRefGoogle Scholar
  11. Herman-Lara E, Santos-Blanco V, Vivar-Vera M, García H, Ochoa-Martínez L, Martínez-Sánchez C (2012) Conjugated linoleic acid content in selected Mexican beef and dairy products. CyTA J Food 10:71–77CrossRefGoogle Scholar
  12. Jenkins T, Wallace R, Moate P, Mosley E (2008) Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J Anim Sci 86:397–412CrossRefGoogle Scholar
  13. Kim Y, Liu R (2002) Increase of conjugated linoleic acid content in milk by fermentation with lactic acid bacteria. J Food Sci 67:1731–1737CrossRefGoogle Scholar
  14. Kim JH, Kim Y, Kim YJ, Park Y (2016) Conjugated linoleic acid: potential health benefits as a functional food ingredient. Annu Rev Food Sci Technol. doi: 10.1146/annurev-food-041715-033028 Google Scholar
  15. Kishino S, Ogawa J, Yokozeki K, Shimizu S (2011) Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme systemrequiring oxidoreduction cofactors. Biosci Biotechnol Biochem 75:318–322CrossRefGoogle Scholar
  16. Lee HY, Park JH, Seok SH, Baek MW, Kim DJ, Lee KE, Paek KS, Lee Y, Park JH (2006) Human originated bacteria Lactobacillus rhamnosus PL60 produce conjugated linoleic acid and show anti-obesity effects in diet-induced obese mice. Biochim Biophys Acta Mol Cell Biol Lipids 1761:736–744CrossRefGoogle Scholar
  17. Lee K, Paek K, Lee H, Park JH, Lee Y (2007) Antiobesity effect of trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice. J Appl Microbiol 103:1140–1146CrossRefGoogle Scholar
  18. Li H, Liu Y, Liu X, Zhang H (2012) Conjugated linoleic acid conversion by six Lactobacillus plantarumstrains cultured in MRS broth supplemented with sunflower oil and soymilk. J Food Sci 77:M330–M336CrossRefGoogle Scholar
  19. Lin TY, Lin CW, Wang YJ (2003) Production of conjugated linoleic acid by enzyme extract of Lactobacillus acidophilus CCRC14079. Food Chem 83:27–31CrossRefGoogle Scholar
  20. Lock AL, Bauman DE (2004) Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids 39:1197–1206CrossRefGoogle Scholar
  21. Macdonald HB (2000) Conjugated linoleic acid and disease prevention: a review of current knowledge. J Am Coll Nutr 19:111S–118SCrossRefGoogle Scholar
  22. Nunes JC, Torres AG (2010) Fatty acid and CLA composition of Brazilian dairy products, and contribution to daily intake of CLA. J Food Compos Anal 23:782–789CrossRefGoogle Scholar
  23. O’Fallon J, Busboom J, Nelson M, Gaskins C (2007) A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J Anim Sci 85:1511–1521CrossRefGoogle Scholar
  24. Ogawa J, Matsumura K, Kishino S, Omura Y, Shimizu S (2001) Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl Environ Microbiol 67:1246–1252CrossRefGoogle Scholar
  25. Özer CO, Kılıç B, Kılıç GB (2016) In vitro microbial production of conjugated linoleic acid by probiotic L. plantarum strains: utilization as a functional starter culture in sucuk fermentation. Meat Sci 114:24–31CrossRefGoogle Scholar
  26. Pospiech A, Neumann B (1995) A versatile quick-prep of genomic DNA from Gram-positive bacteria. TIG 11:217–218CrossRefGoogle Scholar
  27. Puniya AK, Chaitanya S, Tyagi AK, De S, Singh K (2008) Conjugated linoleic acid producing potential of lactobacilli isolated from the rumen of cattle. J Ind Microbiol Biotechnol 35:1223–1228CrossRefGoogle Scholar
  28. Rubio R, Jofre A, Martín B, Aymerich T, Garriga M (2014) Characterization of lactic acid bacteria isolated from infant feces as potential probiotic starter cultures for fermented sausages. Food Microbiol 38:303–311CrossRefGoogle Scholar
  29. Shantha N, Decker E (1993) Conjugated linoleic acid concentrations in processed cheese containing hydrogen donors, iron and dairy-based additives. Food Chem 47:257–261CrossRefGoogle Scholar
  30. Song YL, Kato N, Liu CX, Matsumiya Y, Kato H, Watanabe K (2000) Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group-and species-specific primers derived from the 16S–23S rRNA intergenic spacer region and its flanking 23S rRNA. FEMS Microbiol Lett 187:167–173Google Scholar
  31. Sosa-Castañeda J, Hernández-Mendoza A, Astiazarán-García H, Garcia H, Estrada-Montoya M, González-Córdova A, Vallejo-Cordoba B (2015) Screening of Lactobacillus strains for their ability to produce conjugated linoleic acid in milk and to adhere to the intestinal tract. J Dairy Sci 98:6651–6659CrossRefGoogle Scholar
  32. Taboada N, Van Nieuwenhove C, Alzogaray SL, Medina R (2015) Influence of autochthonous cultures on fatty acid composition, esterase activity and sensory profile of Argentinean goat cheeses. J Food Compos Anal 40:86–94CrossRefGoogle Scholar
  33. Terán V, Pizarro PL, Zacarías M, Vinderola G, Medina R, Van Nieuwenhove C (2015) Production of conjugated dienoic and trienoic fatty acids by lactic acid bacteria and bifidobacteria. J Funct Foods 19:417–425CrossRefGoogle Scholar
  34. Van Nieuwenhove C, Oliszewski R, Gonzalez S, ChaiaA Perez (2007) Conjugated linoleic acid conversion by dairy bacteria cultured in MRS broth and buffalo milk. Lett Appl Microbiol 44:467–474CrossRefGoogle Scholar
  35. Zheng G, Genwang Z, Yan S (2003) Research development of the preparation methods of conjugated linoleic acid. Chem Peking 66:592Google Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  1. 1.Dairy Microbiology DivisionICAR-National Dairy Research InstituteKarnalIndia
  2. 2.College of Dairy Science and TechnologyGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

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