Occurrence of clinical genotype Vibrio vulnificus in clam samples in Mangalore, Southwest coast of India
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Vibrio vulnificus is an opportunistic human pathogen causing gastroenteritis, wound infection and primary septicemia. V. vulnificus population has been divided into subpopulations based on their phenotype and genotype characteristics. In this study, 38.5% (10/26) of clam (Meretrix meretrix) samples obtained from Mangalore markets were seen to harbor V. vulnificus. Biochemical characterization of V. vulnificus isolates showed the strains to belong to Biotype 1 phenotype. Genotyping of strains using the 16S rRNA and virulence correlated gene (vcg) typing methods identified the isolates to be of 16S rRNA typeB and vcgC type respectively. Analysis of representative 16S rRNA and vcg gene sequences further substantiated that the V. vulnificus associated with clams in the present study to be of clinical origin, implicated as virulent type responsible for causing infection in humans.
KeywordsVibrio vulnificus Clinical genotype Biotype 1 vcgC gene 16S rRNA typeB Clams
The first author acknowledges the funding received by UGC Rajiv Gandhi National Fellowship Scheme (RGNF-2013-14-SC-KER-48760) to carry out this study. The authors acknowledge the DBT-Bioinformatics Centre, KVAFSU, College of Fisheries Mangalore for carrying out the sequence analysis work.
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