Journal of Food Science and Technology

, Volume 54, Issue 3, pp 669–678 | Cite as

Effects of degree of hydrolysis (DH) on the functional properties of egg yolk hydrolysate with alcalase

  • Zhi-jie Bao
  • Ying Zhao
  • Xiao-ying Wang
  • Yu-Jie Chi
Original Article


Effects of enzymatic hydrolysis on the physicochemical and functional properties of egg yolk were investigated in this study. Alcalase, neutrase and flavourzyme were used to hydrolyze egg yolk. Solubility, foaming properties, emulsifying and microstructure properties of egg yolk were determined after enzymatic hydrolysis. Results showed that alcalase had better efficiency of hydrolysis than neutrase and flavourzyme. Enzymatic hydrolysis caused a marked changes in protein solubility, surface hydrophobicity, molecular weight distributions, microstructure and other functional properties. It was observed that egg yolk and its hydrolysates exhibited a relatively smooth curve over the entire pH range; egg yolk hydrolysates with high DH had higher solubility than those having lower DH. Foam capacity and stability generally increased with increasing DH although foam stability showed a decrease at 15% DH. Hydrolysates of egg yolk showed scattered and fewer aggregated particles. This study demonstrated that egg yolk hydrolysates could be an excellent emulsifying agent for food and other applications.


Egg yolk Enzyme Hydrolysate Functional properties 



This study was funded by the Modern Agro-industry Technology Research System of China (Grant No. CARS-41-K25) and National Natural Science Foundation of China (Grant No. 31470094). The authors also sincerely appreciate the invaluable inputs by the editors and reviewers which greatly improved the quality of the manuscript.


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Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  • Zhi-jie Bao
    • 1
  • Ying Zhao
    • 1
  • Xiao-ying Wang
    • 1
  • Yu-Jie Chi
    • 1
  1. 1.Department of Food ScienceNortheast Agricultural UniversityHarbinChina

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