Journal of Food Science and Technology

, Volume 54, Issue 3, pp 601–610 | Cite as

Single step extraction and derivatization of intramuscular lipids for fatty acid Ultra Fast GC analysis: application on pig thigh

  • Eleonora Laura De Paola
  • Giuseppe Montevecchi
  • Francesca Masino
  • Andrea Antonelli
  • Domenico Pietro Lo Fiego
Original Article


Two different methods for single step transesterification from pig meat without fat extraction have been tested. Freeze-drying of the meat with and without anhydrous salt, followed by a base-catalyzed transmethylation (KOH/MeOH) was carried out. Both methods were compared with the standard Folch procedure of fat extraction followed by transmethylation. The methods were tested on a complete sample set of biceps femoris of pig thigh, used for the production of dry-cured ham. The set was divided in three subgroups according to total fat content. Both derivatization protocols on freeze-dried pork muscle were proven to be a valid alternative to the Folch procedure for FAME analysis. Freeze-drying method offered several advantages in comparison with the Folch procedure, including a lower solvent requirement, and process temperature, as well as considerable saving of time. In freeze-drying, the addition of an anhydrous salt (Na2SO4) gave more friable samples which resulted in higher yields for some fatty acids, particularly evident in the case of tissues with high lipid content.


Fatty acids analysis Meat lipids Single step transesterification Ultra Fast GC 



The research was supported by a financial contribution from MIUR-PRIN 2007 (Prof. Domenico Pietro Lo Fiego).


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Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  1. 1.Dipartimento di Scienze della Vita (Area: Scienze Agro-Alimentari)Università degli Studi di Modena e Reggio EmiliaReggio EmiliaItaly
  2. 2.Centro di Ricerca Interdipartimentale per il Miglioramento e la Valorizzazione delle Risorse Biologiche Agro-Alimentari BIOGEST-SITEIAUniversità degli Studi di Modena e Reggio Emilia, Tecnopolo di Reggio EmiliaReggio EmiliaItaly

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