Journal of Food Science and Technology

, Volume 54, Issue 3, pp 601–610 | Cite as

Single step extraction and derivatization of intramuscular lipids for fatty acid Ultra Fast GC analysis: application on pig thigh

  • Eleonora Laura De Paola
  • Giuseppe Montevecchi
  • Francesca Masino
  • Andrea Antonelli
  • Domenico Pietro Lo Fiego
Original Article
  • 135 Downloads

Abstract

Two different methods for single step transesterification from pig meat without fat extraction have been tested. Freeze-drying of the meat with and without anhydrous salt, followed by a base-catalyzed transmethylation (KOH/MeOH) was carried out. Both methods were compared with the standard Folch procedure of fat extraction followed by transmethylation. The methods were tested on a complete sample set of biceps femoris of pig thigh, used for the production of dry-cured ham. The set was divided in three subgroups according to total fat content. Both derivatization protocols on freeze-dried pork muscle were proven to be a valid alternative to the Folch procedure for FAME analysis. Freeze-drying method offered several advantages in comparison with the Folch procedure, including a lower solvent requirement, and process temperature, as well as considerable saving of time. In freeze-drying, the addition of an anhydrous salt (Na2SO4) gave more friable samples which resulted in higher yields for some fatty acids, particularly evident in the case of tissues with high lipid content.

Keywords

Fatty acids analysis Meat lipids Single step transesterification Ultra Fast GC 

Notes

Acknowledgements

The research was supported by a financial contribution from MIUR-PRIN 2007 (Prof. Domenico Pietro Lo Fiego).

References

  1. AOAC (2012) Association of Official Analytical Chemist. Official methods of analysis of the association of official analytical chemists, 19th edn. AOAC, ArlingtonGoogle Scholar
  2. AOAC Method 24.005 (1990a). Standard method for crude lipids determination or ethereal extract. Official methods of analysis, Association of Official Analytical Chemists, 15th ed. AOAC, Arlington, VA, USAGoogle Scholar
  3. AOAC 960.39 (1990b). Standard method for crude lipids determination or ethereal extract. Official methods of analysis,15th ed. AOAC, Arlington, VA, USA Google Scholar
  4. Berg H, Magard M, Johansson G, Mathiasson L (1997) Development of an SFE method for determination of lipid classes and total fat in meats and its comparison with conventional methods. J Chromatogr A 785:345–352CrossRefGoogle Scholar
  5. Boselli E, Velazco V, Caboni MF, Lercker G (2001) Pressurized liquid extraction of lipids for the determination of oxysterols in egg-containing food. J Chromatogr A 917:239–244CrossRefGoogle Scholar
  6. Calvo P, Castano AL, Hernandez MT, Gonzalez-Gomez D (2011) Effects of microcapsule constitution on the quality of microencapsulated walnut oil. Eur J Lipid Sci Technol 113:1273–1280CrossRefGoogle Scholar
  7. Christie W (1993) Advances in lipid methodology—two. The Oily Press Ltd, DundeeGoogle Scholar
  8. Christie W (2003) Lipid analysis: isolation, separation, identification and structural analysis of lipids, 3rd edn. American Oil Chemists’ Society, UrbanaGoogle Scholar
  9. Destaillats F, Golay PA, Joffre F, De Wispelaere M, Hug B, Giuffrida F, Fauconnot L, Dionisi F (2007) Comparison of available analytical methods to measure trans-octadecenoic acid isomeric profile and content by gas-liquid chromatography in milk fat. J Chromatogr A 1145:222–228CrossRefGoogle Scholar
  10. Dodds ED, McCoy MR, Geldenhuys A, Rea LD, Kennish JM (2004) Microscale recovery of total lipids from fish tissue by accelerated solvent extraction. J Am Oil Chem Soc 81:835–840CrossRefGoogle Scholar
  11. Ficarra A, Lo Fiego DP, Minelli G, Antonelli A (2010) Ultra fast analysis of subcutaneous pork fat. Food Chem 121:809–814CrossRefGoogle Scholar
  12. Ficarra A, De Paola EL, Lo Fiego DP, Minelli G, Antonelli A (2013) Single step extraction and derivatization of meat lipids for fatty acid Ultra Fast GC analysis. Riv Ital Sostanze Grasse 90:81–86Google Scholar
  13. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
  14. Golay PA, Dionisi F, Hug B, Giuffrida F, Destaillats F (2006) Direct quantification of fatty acids in dairy powders with special emphasis on trans fatty acid content. Food Chem 101:1115–1120CrossRefGoogle Scholar
  15. Grahl-Nielsen O (1999) Comment: fatty acid signatures and classification trees: new tools for investigating the foraging ecology of seals. Can J Fish Aquat Sci 56:2219–2223CrossRefGoogle Scholar
  16. Grob K, Suter B (2000) Determination of total fat, milk fat and fatty acid composition through 1-min transesterification directly in the foods: collaborative studies. Mitt Geb Lebensmittelunters Hyg 91:224–233Google Scholar
  17. Joensen H, Grahl-Nielsen O (2001) The redfish species Sebastes viviparus, Sebastes marinus and Sebastes mentella have different composition of their tissue fatty acids. Comp Biochem Phys B 129:73–85CrossRefGoogle Scholar
  18. Kaiser HF (1958) The varimax criterion for analytic rotation in factor analysis. Psychometrika 23:187–200CrossRefGoogle Scholar
  19. Kolakowska A, Zygadlik B, Czarnecki H, Szczygielski M (1998) Susceptibility of muscle lipids in pigs to oxidation depending on breed soybean or rapeseed diet. Pol J Food Nutr Sci 7:655–662Google Scholar
  20. Lee MRF, Tweed JKS, Kim EJ, Scollan ND (2012) Beef, chicken and lamb fatty acid analysis—a simplified direct bimethylation procedure using freeze-dried material. Meat Sci 92:863–866CrossRefGoogle Scholar
  21. List GR, Friedrich JP, King JW (1989) Supercritical CO2 extraction and processing of oilseeds. Oil Mill Gaz 95:28–34Google Scholar
  22. Lo Fiego DP (1996) Carcass fatness and lipid quality in the heavy pig. Meat Focus Int 5:261–263Google Scholar
  23. Lo Fiego DP, Santoro P, Macchioni P, De Leonibus E (2005) Influence of genetic type, live weight at slaughter and carcass fatness on fatty acid composition of subcutaneous adipose tissue of raw ham in the heavy pig. Meat Sci 69:107–114CrossRefGoogle Scholar
  24. Lo Fiego DP, Macchioni P, Minelli G, Santoro P (2010) Lipid composition of covering and intramuscular fat in pigs at different slaughter age. Ital J Anim Sci 9:200–205CrossRefGoogle Scholar
  25. Merkle JA, Larick DK (1995) Fatty acid content of supercritical carbon dioxide-extracted fractions of beef fat. J Food Sci 60:959–962CrossRefGoogle Scholar
  26. Nieto G, Ros G (2012) Modification of fatty acid composition in meat through diet: effect on lipid peroxidation and relationship to nutritional quality—a review. In: Catala A (ed) Lipid Peroxidation, chapter 12, In Tech Open Access Publisher ,Rijeka, pp 239–258. http://www.intechopen.com/books/lipid-peroxidation/modification-of-fatty-acid-composition-in-meat-through-diet-effect-on-lipid-peroxidation-and-relatio
  27. Raventós M, Duarte S, Alarcón R (2002) Application and possibilities of supercritical CO2 extraction in food processing industry: an overview. Food Sci Technol Int 8:269–284CrossRefGoogle Scholar
  28. Rossi R, Corino C (2002) Influence of long-term nutrition with different dietary fats on fatty acid composition of heavy pigs backfat. Ital J Anim Sci 1:7–16CrossRefGoogle Scholar
  29. Sahena F, Zaidul ISM, Jinap S, Karim AA, Abbas KA, Norulaini NAN, Omar AKM (2009) Application of supercritical CO2 in lipid extraction—a review. J Food Eng 95:240–253CrossRefGoogle Scholar
  30. Schäfer K (1998) Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material. Anal Chim Acta 358:69–77CrossRefGoogle Scholar
  31. Suter B, Grob K, Paciarelli B (1997a) Determination of fat content and fatty acid composition through 1-min transesterification in the food sample; principle. Z Lebensm Unters F A 204:252–258CrossRefGoogle Scholar
  32. Suter B, Grob K, Paciarelli B, Novoselac A (1997b) Determination of fat content and fatty acid composition through 1-min transesterification in the food sample. II. Solubilization of the fat, results. Mitt Geb Lebensmittelunters Hyg 88:259–276Google Scholar
  33. Suter B, Grob K, Paciarelli B (1999) Simultaneous determination of milk fat (butiryc acid) and total fat by 1-min transesterification directly in the food. Mitt Geb Lebensmittelunters Hyg 90:149–166Google Scholar
  34. Taylor DL, Larick DK (1995) Investigations into the effect of supercritical carbon dioxide extraction on the fatty acid and volatile profiles of cooked chicken. J Agric Food Chem 43:2369–2374CrossRefGoogle Scholar
  35. Wood JD, Richardson RI, Nute GR, Fisher AV, Campo MM, Kasapidou E, Sheard PR, Enser M (2004) Effects of fatty acids on meat quality: a review. Meat Sci 66:21–32CrossRefGoogle Scholar
  36. Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Sci 78:343–358CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2017

Authors and Affiliations

  1. 1.Dipartimento di Scienze della Vita (Area: Scienze Agro-Alimentari)Università degli Studi di Modena e Reggio EmiliaReggio EmiliaItaly
  2. 2.Centro di Ricerca Interdipartimentale per il Miglioramento e la Valorizzazione delle Risorse Biologiche Agro-Alimentari BIOGEST-SITEIAUniversità degli Studi di Modena e Reggio Emilia, Tecnopolo di Reggio EmiliaReggio EmiliaItaly

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