Journal of Food Science and Technology

, Volume 53, Issue 6, pp 2804–2816 | Cite as

Phytochemical study and antioxidant, antimicrobial and anticancer activities of Melanelia subaurifera and Melanelia fuliginosa lichens

  • Svetlana Ristić
  • Branislav Ranković
  • Marijana KosanićEmail author
  • Tatjana Stanojković
  • Slaviša Stamenković
  • Perica Vasiljević
  • Ivana Manojlović
  • Nedeljko Manojlović
Original Article


The aim of this study was to investigate antioxidant, antimicrobial and anticancerous activity of Melanelia subaurifera and Melanelia fuliginosa. The phytochemical analysis was determined by HPLC–UV method. Antioxidant activity was evaluated by DPPH and reducing power assay while antimicrobial activity was determined by minimal inhibitory concentration. The cytotoxic activity was tested using MTT method. The method for quantification of 2′-O-methyl anziaic acid and lecanoric acid in these lichens using RF-HPLC was also developed and validated. The depsides (lecanoric acid, gyrophoric acid, atranorin, anziaic acid and 2′-O-methyl anziaic acid), and dibenzofurane (usnic acid) were identified in these lichens. The antioxidant activity (IC50) of lichens extracts ranged from 121.52 to 424.51 μg/ml. 2′-O-Methyl anziaic acid showed the highest antimicrobial activity with MIC ranging from 0.0625 to 1 mg/ml. M. subaurifera extract showed the highest cytotoxic activity against the tested cell lines (IC50 = 9.88 to 31.64 μg/ml).


HPLC analysis Lichens secondary metabolites Biological activities 



This work was financed in part by the Ministry of Science, Technology, and Development of the Republic of Serbia and was carried out within the framework of Project Nos. 173032, 175011 and 172015.


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Copyright information

© Association of Food Scientists & Technologists (India) 2016

Authors and Affiliations

  • Svetlana Ristić
    • 1
  • Branislav Ranković
    • 1
  • Marijana Kosanić
    • 1
    Email author
  • Tatjana Stanojković
    • 2
  • Slaviša Stamenković
    • 3
  • Perica Vasiljević
    • 3
  • Ivana Manojlović
    • 4
  • Nedeljko Manojlović
    • 4
  1. 1.Department of Biology, Faculty of ScienceUniversity of KragujevacKragujevacSerbia
  2. 2.Institute of Oncology and Radiology of SerbiaBelgradeSerbia
  3. 3.Department of Biology and Ecology, Faculty of Sciences and MathematicsUniversity of NišNisSerbia
  4. 4.Department of Pharmacy, Faculty of Medicinal SciencesUniversity of KragujevacKragujevacSerbia

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