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Journal of Food Science and Technology

, Volume 53, Issue 6, pp 2804–2816 | Cite as

Phytochemical study and antioxidant, antimicrobial and anticancer activities of Melanelia subaurifera and Melanelia fuliginosa lichens

  • Svetlana Ristić
  • Branislav Ranković
  • Marijana KosanićEmail author
  • Tatjana Stanojković
  • Slaviša Stamenković
  • Perica Vasiljević
  • Ivana Manojlović
  • Nedeljko Manojlović
Original Article

Abstract

The aim of this study was to investigate antioxidant, antimicrobial and anticancerous activity of Melanelia subaurifera and Melanelia fuliginosa. The phytochemical analysis was determined by HPLC–UV method. Antioxidant activity was evaluated by DPPH and reducing power assay while antimicrobial activity was determined by minimal inhibitory concentration. The cytotoxic activity was tested using MTT method. The method for quantification of 2′-O-methyl anziaic acid and lecanoric acid in these lichens using RF-HPLC was also developed and validated. The depsides (lecanoric acid, gyrophoric acid, atranorin, anziaic acid and 2′-O-methyl anziaic acid), and dibenzofurane (usnic acid) were identified in these lichens. The antioxidant activity (IC50) of lichens extracts ranged from 121.52 to 424.51 μg/ml. 2′-O-Methyl anziaic acid showed the highest antimicrobial activity with MIC ranging from 0.0625 to 1 mg/ml. M. subaurifera extract showed the highest cytotoxic activity against the tested cell lines (IC50 = 9.88 to 31.64 μg/ml).

Keywords

HPLC analysis Lichens secondary metabolites Biological activities 

Notes

Acknowledgments

This work was financed in part by the Ministry of Science, Technology, and Development of the Republic of Serbia and was carried out within the framework of Project Nos. 173032, 175011 and 172015.

References

  1. Behera BC, Verma N, Sonone A, Makhija U (2009) Optimization of culture conditions for lichen Usnea ghattensis G. awasthi to increase biomass and antioxidant metabolite production. Food Technol Biotechnol 47:7–12Google Scholar
  2. Berdy J (2012) Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 65:385–395CrossRefGoogle Scholar
  3. Bezivin C, Tomasi S, Lohezic-Le Devehat F, Boustie C (2003) Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. Phytomedicine 10:499–503CrossRefGoogle Scholar
  4. Blanco O, Crespo A, Divakar PK, Esslinger TL, Hawksworth DL, Lumbsch HT (2004) Melanelixia and Melanohalea, two new genera segregated from Melanelia (Parmeliaceae) based on molecular and morphological data. Mycol Res 108:873–884CrossRefGoogle Scholar
  5. Bnyan I, Hasan H, Ewadh M (2013) Antimicrobial activity of Citrullus colocynthis against different types of bacteria. Adv Life Sci Technol 7:48–51Google Scholar
  6. Bogo D, de Matos MF, Honda NK, Pontes EC, Oguma PM, da Santos EC et al (2010) In vitro antitumour activity of orsellinates. Z Naturforsch C 65:43–48CrossRefGoogle Scholar
  7. Boustie J, Tomasi S, Grube M (2011) Bioactive lichen metabolites: alpine habitats as an untapped source. Phytochem Rev 10:287–307CrossRefGoogle Scholar
  8. Cheng B, Cao S, Vasquez V, Annamalai T, Tamayo-Castillo G, Clardy J et al (2013) Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor. PLoS ONE 8:e60770CrossRefGoogle Scholar
  9. Culberson CF, Hertel H (1972) 2′-O-methylanziaic acid, a new depside in Lecidea diducens and Lecidea speirodes. The Brylogist 75:372–376CrossRefGoogle Scholar
  10. Dobson FS (2011) Lichens. An illustrated guide to the British and Irish species, 6th edn. Richmond Publishing Co, LondonGoogle Scholar
  11. Dorman HJ, Bachmayer O, Kosar M, Hiltunen R (2004) Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in Turkey. J Agric Food Chem 52:762–770CrossRefGoogle Scholar
  12. Dos Santos FM, de Souza MG, Crotti AE, Martins CH, Ambrósio SR, Veneziani RC, Silva EML, Cunha WR (2012) Evaluation of antimicrobial activity of extracts of Tibouchina candolleana (melastomataceae), isolated compounds and semi-synthetic derivatives against endodontic bacteria. Braz J Microbiol 43:793–799CrossRefGoogle Scholar
  13. Hisham AA (2014) Comparative antibacterial and antibiofilm activities of manuka honey and Egyptian clover honey. Asian J Appl Sci 2:110–115Google Scholar
  14. Huneck S, Yoshimura I (1996) Identification of lichen substances. Springer, Berlin HeidelbergCrossRefGoogle Scholar
  15. Jayabalan R, Subathradevi P, Marimuthu S, Sathishkumar M, Swaminathan K (2008) Changes in free-radical scavenging ability of kombucha tea during fermentation. Food Chem 109:227–234CrossRefGoogle Scholar
  16. Jayaprakasha GK, Rao LJ (2000) Phenolic constituents from the lichen Parmotrema stuppeum (Nyl.). Hale and their antioxidant activities. Z Naturforsch C 55:1018–1022Google Scholar
  17. Karagoz A, Dogruoz N, Zeybek Z, Aslan A (2009) Antibacterial activity of some lichen extracts. J Med Plants Res 3:1034–1039Google Scholar
  18. Kosanić M, Ranković B, Stanojković T (2012) Antioxidant, antimicrobial, and anticancer activities of three Parmelia species. J Sci Food Agric 92:1909–1916CrossRefGoogle Scholar
  19. Lin H, Annamalai T, Bansod P, Tse-Dinhb Y, Sun D (2013) Synthesis and antibacterial evaluation of anziaic acid and analogues as topoisomerase I inhibitors. Med Chem Commun 4:1613–1618CrossRefGoogle Scholar
  20. Lopes TI, Coelho RG, Yoshida NC, Honda NK (2008) Radicalscavenging activity of orsellinates. Chem Pharm Bull 56:1151–1554CrossRefGoogle Scholar
  21. Manojlović N, Vasiljević P, Jusković M, Najman S, Janković S, Milenković-Andjelković A (2010) HPLC analysis and cytotoxic potential of extracts from the lichen, Thamnolia vermicularis var. Subuliformis. J Med Plants Res 4:817–823Google Scholar
  22. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRefGoogle Scholar
  23. NCCLS (National Committee for Clinical Laboratory Standards) (1998) Reference method for broth dilution antifungal susceptibility testing of conidium-forming filamentous fungi: Proposed standard M38-p. NCCLS, WayneGoogle Scholar
  24. Oyaizu M (1986) Studies on products of browning reaction prepared from glucoseamine. Jpn J Nutr 44:307–314CrossRefGoogle Scholar
  25. Saran S, Menon S, Shailajan S, Pokharna P (2013) Validated RP-HPLC method to estimate eugenon from commercial formulations like Caturjata Churna, Lavangadi Vati, Jatiphaladi Churna, Sitopaladi Churna and clove oil. J Pharm Res 6:53–60Google Scholar
  26. Sarker SD, Nahar L, Kumarasamy Y (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42:321–324CrossRefGoogle Scholar
  27. Schinor EC, Salvador MJ, Ito IY (2007) Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chresta scapigera. Braz J Microbiol 38:145–149CrossRefGoogle Scholar
  28. Sharnoff SD (1997) Lichens and people. Online, http://www.lichen.com/people.html
  29. Shukla V, Joshi G, Rawat M (2010) Lichens as a potential natural source of bioactive compounds: a review. Phytochem Rev 9:303–314CrossRefGoogle Scholar
  30. Slinkard K, Singleton VL (1997) Total phenolic analyses: automation and comparison with manual method. Am J Enol Vitic 28:49–55Google Scholar
  31. Swathi D, Suchitha Y, Prashith Kekuda TR, Venugopal TM, Vinayaka KS, Mallikarjun N et al (2010) Antimicrobial, antihlmintic and insecticidal activity of a macrolichen Everniastrum cirrhatum (FR.). Hale. IJDDR 2:780–789Google Scholar
  32. Vasudeo PZ, Lew C (2011) Biopharmaceutical potential of lichens. Pharm Biol 50:778–798Google Scholar
  33. Wirth V (1995) Die Flechten Baden-Würtembergs, Verbreitungsatlas, 1and2. Eugen Ulmer GmbHandCo, StuttgartGoogle Scholar
  34. Yoshimura I, Kinoshita Y, Yamamoto Y, Huneck S, Yamada Y (1994) Analysis of secondary metabolites from lichen by high performance liquid chromatography with a photodiode array detector. Phytochem Anal 5:197–205CrossRefGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2016

Authors and Affiliations

  • Svetlana Ristić
    • 1
  • Branislav Ranković
    • 1
  • Marijana Kosanić
    • 1
    Email author
  • Tatjana Stanojković
    • 2
  • Slaviša Stamenković
    • 3
  • Perica Vasiljević
    • 3
  • Ivana Manojlović
    • 4
  • Nedeljko Manojlović
    • 4
  1. 1.Department of Biology, Faculty of ScienceUniversity of KragujevacKragujevacSerbia
  2. 2.Institute of Oncology and Radiology of SerbiaBelgradeSerbia
  3. 3.Department of Biology and Ecology, Faculty of Sciences and MathematicsUniversity of NišNisSerbia
  4. 4.Department of Pharmacy, Faculty of Medicinal SciencesUniversity of KragujevacKragujevacSerbia

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