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Anthocyanins-rich extract of wild Chinese blueberry protects glucolipotoxicity-induced INS832/13 β-cell against dysfunction and death

Journal of Food Science and Technology volume 52, pages 3022–3029 (2015)Cite this article

Abstract

As commonly observed events in diabetic patients, glucolipotoxicity induces oxidative stress, apoptosis and functional defects in β-cells. Anthocyanins are well investigated as strong antioxidants and modulators for metabolic syndromes. Therefore, this study examined the protective effects of anthocyanins-rich extracts (BAE) from wild Chinese blueberry (Vaccinium spp.) against glucolipotoxicity in β-cells. Results showed that INS832/13 β-cells subjected to glucolipotoxicity were significantly decreased (p < 0.05) in cell survival rate, which were alleviated by BAE and metformin treatments. Both BAE and metformin reduced reactive oxidative species and improved the antioxidant defense system. Moreover, BAE were effective in reducing intracellular triglycerides (TG) level, restoring intracellular insulin content, lowering basal insulin secretion (BIS) and increasing glucose-stimulated insulin secretion which in turn resulted in an elevated insulin secretion index. However, metformin only demonstrated marginal effect on secretion dysfunction and had no effect (p > 0.05) on BIS or TG. Additionally, TG levels reduced by BAE treatment were correlated with BIS (p < 0.01, r = 0.9755). This study has for the first time demonstrated that anthocyanin enriched extract of wild Chinese blueberry could effectively protect β-cells against glucolipotoxicity in vitro. These results implied the potential efficacy of BAE as a complementary measure for diabetes intervention.

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Acknowledgments

We thank Dr. Christopher Newgard (Duke University) for providing the INS-1 832/13 cells. This research was supported in part by the Key Projects of China in the National Science & Technology Pillar Program, holding by Baoping Ji, during the Twelfth Five-Year Plan Period grand (2011BAD08B03-01).

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  1. Baoping Ji

    Present address: China Agricultural University, Mailbox #294, #17 Qinghua East Road, Haidian District, Beijing, 100083, People’s Republic of China

Authors and Affiliations

  1. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China

    Jia Liu, Fengyi Gao, Baoping Ji, Ruojun Wang, Junsi Yang, Huijun Liu & Feng Zhou

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  1. Jia Liu
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  2. Fengyi Gao
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Correspondence to Baoping Ji.

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Liu, J., Gao, F., Ji, B. et al. Anthocyanins-rich extract of wild Chinese blueberry protects glucolipotoxicity-induced INS832/13 β-cell against dysfunction and death. J Food Sci Technol 52, 3022–3029 (2015). https://doi.org/10.1007/s13197-014-1379-6

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