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Efficacy of Angelica archangelica essential oil, phenyl ethyl alcohol and α- terpineol against isolated molds from walnut and their antiaflatoxigenic and antioxidant activity

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Abstract

Mold association, aflatoxin B1 contamination as well as oxidative deterioration of agri-food items during storage and processing are some global task for food industries. In view of the adverse effects of some synthetic preservatives on treated food items and subsequently on consumers health, recently plant based chemicals are encouraged by food industries as better alternatives of synthetics. The present study recommends the combination (1:1:1) of Angelica archangelica essential oil: Phenyl ethyl alcohol (PEA): α- terpineol as botanical preservative against molds, aflatoxin contamination and oxidative deterioration of walnut samples. Eight mold species were procured from stored walnut samples, including some aflatoxigenic Aspergillus flavus strains. The combination inhibited growth of aflatoxigenic strain Aspergillus flavus NKDW-7 and aflatoxin B1 production at 2.25 and 2.0 μL mL−1 respectively. The IC50 value of the combination was recorded as 3.89 μL mL−1, showing strong antioxidant potential. The antifungal action of the combination showed > 90 % decrease in ergosterol content in plasma membrane of A. flavus at 2.0 μL mL−1. The LD50 of the combination, through oral administration on mice, was 9562.9 μL kg−1 body weight, indication favourable safety profile as a plant based preservative. The combination may be recommended as safe preservative against molds, aflatoxin contamination and oxidative deterioration of walnut samples.

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Acknowledgment

Bhanu Prakash is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India for financial assistance as Senior Research Fellow (SRF) and Dr. Priyanka Singh is thankful to Department of Science and Technology (DST) New Delhi for financial assistance as DST- Fast Track Young Scientist.

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Authors and Affiliations

  1. Laboratory of Herbal Pesticides, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, U.P., India

    Bhanu Prakash, Priyanka Singh & N. K. Dubey

  2. Department of Botany, University of Jammu, Jammu, 180006, J&K, India

    Reema Goni & Ajay Kumar Pandit Raina

Authors
  1. Bhanu Prakash
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  2. Priyanka Singh
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  3. Reema Goni
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  4. Ajay Kumar Pandit Raina
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  5. N. K. Dubey
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Correspondence to N. K. Dubey.

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Prakash, B., Singh, P., Goni, R. et al. Efficacy of Angelica archangelica essential oil, phenyl ethyl alcohol and α- terpineol against isolated molds from walnut and their antiaflatoxigenic and antioxidant activity. J Food Sci Technol 52, 2220–2228 (2015). https://doi.org/10.1007/s13197-014-1278-x

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  • DOI: https://doi.org/10.1007/s13197-014-1278-x

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