Abstract
The changes in phytate, phytase activity and in vitro availability of iron and zinc during soaking and sprouting of green and white faba bean (Vicia faba L.) were investigated. Faba bean were soaked for 24 h and germinated for 72 h after soaking for 24 h to reduce phytate content and increase iron and zinc in vitro availability. The results revealed that iron and zinc content was significantly reduced from 28.2 to 39.8 % and 12.5 to 27.6 % for soaking treatment and 38.2 to 38.9 % and 24.5 to 29.2 % for sprouting treatment, respectively. Phytate content was significantly reduced from 26.9 to 32.5 % for soaking treatment and 28.0 to 34.9 % for sprouting treatment, respectively. The results proved that the main distinct point is the change of phytase activity as well as specific activity during different treatment which showed no significant differences between the green and white faba bean. The in vitro availability of iron and zinc were significantly improved as a result of soaking and sprouting treatments.
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References
Adeyeye EI, Arogundade LA, Akintayo ET, Aisida OA, Alao PA (2000) Calcium, zinc and phytate interrelationships in some foods of major consumption in Nigeria. Food Chem 71:435–441
Barrientos L, Scott JJ, Murthy PPN (1994) Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen. Plant Physiol 106:1489–1495
Bau HM, Villaume C, Nicolas JP, Mejean L (1997) Effects of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean seeds. J Sci Food Agric 73:1–9
Egli I, Davidsson L, Juillerat MA, Barclay D, Hurrell RF (2002) The influence of soaking and germination on the phytase activity and phytic acid content of grains and seeds potentially useful for complementary feeding. J Food Sci 67:3484–3488
Elkhalil EAI, El Tinay AH, Mohamed BE, Elsheikh EAE (2001) Effect of malt pretreatment on phytic acid and in vitro protein digestibility of sorghum flour. Food Chem 72:29–32
Godoy S, Chicco C, Meschy F, Requena F (2005) Phytic phosphorus and phytase activity of animal feed ingredients. Commun Rep 30:24–28
Greiner R, Konietzny U (1999) Improving enzymatic reduction of myo-inositol phosphates with inhibitory effects on mineral absorption in black beans (Phaseolus vulgaris var. Preto). J Food Process Preserv 23:249–261
Greiner R, Konietzny U (2006) Phytase for food application. Food Technol Biotechnol 44:125–140
Haug G, Lantzsch W (1983) Methods for determination of phytate of cereal products. J Sci Food Agric 34:1423–1424
Horwitz W (2000) Official Methods of Analysis of AOAC International. Association of Official Analytical Chemists, AOAC, Washington, D.C.
Kayode PAP, Linnemann AR, Nout MJR, Hounhouigan DJ, Stomph TJ (2006) Diversity and food quality properties of farmers’ varieties of sorghum from Benin. J Sci Food Agric 86:1032–1039
Kayode APP, Hounhouigana JD, Nout MJR (2007) Impact of brewing process operations on phytate, phenolic compounds and in vitro solubility of iron and zinc in opaque sorghum beer. LWT Food Sci Technol 40:834–841
Kiers LJ, Nout MJR, Rombouts FM (2000) In vitro digestibility of processed and fermented soya bean, cowpea and maize. J Sci Food Agric 80:1325–1331
Kumar V, Sinha AK, Makkar HPS, Becker K (2010) Dietary roles of phytate and phytase in human nutrition: a review. Food Chem 120:945–959
Lestienne I, Icard-Verniere C, Mouquet C, Picq C, Treche S (2005) Effects of soaking whole cereal and legume seeds on iron, zinc, and phytate contents. Food Chem 89:421–425
Lorenz K (1980) Cereal sprouts: composition, nutritive value, food applications. Crit Rev Food Sci Nutr 13:353–385
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 187:265–257
Luo YW, Gu ZX, Han YH, Chen ZG (2009) The impact of processing on phytic acid, in vitro soluble iron and Phy/Fe molar ratio of faba bean(Vicia faba L.). J Sci Food Agric 89:861–866
Luo YW, Xie WH, Cui QX (2010) Effects of phytases and dehulling treatments on in vitro iron and zinc bioavailability in faba bean (Vicia faba L.) flour and legume fractions. J Food Sci 75:c191–c198
Ma G, Jin Y, Piao J, Kok F, Bonnema G, Jacobsen E (2005) Phytate, calcium, iron, and zinc contents and their molar ratios in food commonly consumed in China. J Agric Food Chem 53:10285–10290
Marero LM, Payumo EM, Aguinaldo AR, Matsumoto I, Homma S (1991) The antinutritional factors in weaning foods prepared from germinated legumes and cereals. Lebensm Wiss Technol 24:177–181
Mubarak AE (2005) Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem 89:489–495
Radhakrishnan MR, Sivaprasad J (1980) Tannin content of sorghum varieties and their role in iron bioavailability. J Agric Food Chem 28:55–57
Reddy NR, Sathe SK (2002) Occurrence, distribution, content, and dietary intake of phytate. In: Food phytates. CRC Press, Boca Raton
Sandberg AS, Svanberg U (1991) Phytate hydrolysis by phytase in cereals; effects on in vitro estimation of iron availability. J Food Sci 56:1330–1333
Steiner T, Mosenthin R, Zimmermann B, Greiner R, Roth S (2007) Distribution of total phosphorus, phytate phosphorus and phytase activity in legume seeds, cereals and cereal by-products as influenced by harvest year and cultivar. Anim Feed Sci Technol 133:320–334
Towo E, Matuschek E, Svanberg U (2006) Fermentation and enzyme treatment of tannin sorghum gruels: effect of phenolic compounds, phytate and in vitro accessible iron. Food Chem 94:369–376
Troost FJ, Brummer RJ, Dainty JR, Hoogewerff JA, Bull VJ (2003) Iron supplements inhibit zinc but not copper absorption in vivo in ileostomy subjects. Am J Clin Nutr 78:1018–1023
Vijayakumari K, Pugalenthi M, Vadivel V (2007) Effect of soaking and hydrothermal processing methods on the levels of antinutrients and in vitro protein digestibility of Bauhinia purpurea L. seeds. Food Chem 103:968–975
Zielinski H, Frias M, Mariusz K, Kozlowska PH, Vidal-Valverde C (2005) Vitamin B1 and B2, dietary fiber and mineral content of cruciferae sprouts. Eur Food Res Technol 221:78–83
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This work was supported by National Science Foundation of China (31201318) and Qing Lan Project.
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Luo, Y., Xie, W. Effect of soaking and sprouting on iron and zinc availability in green and white faba bean (Vicia faba L.). J Food Sci Technol 51, 3970–3976 (2014). https://doi.org/10.1007/s13197-012-0921-7
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DOI: https://doi.org/10.1007/s13197-012-0921-7
Keywords
- Soaking
- Sprouting
- Faba bean
- Iron
- Zinc availability