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Journal of Food Science and Technology

, Volume 51, Issue 9, pp 2006–2013 | Cite as

Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology

  • Weibao Kong
  • Na Liu
  • Ji Zhang
  • Qi Yang
  • Shaofeng Hua
  • Hao Song
  • Chungu Xia
Original Article

Abstract

An investigation into ultrasound-assisted extraction (UAE) was conducted for the extraction of chlorophyll from Chlorella vulgaris residue after lipid separation. The best possible combination of extraction parameters was obtained with the response surface methodology (RSM), at a three-variable, three-level experiment Box–Behnken design (BBD). The optimum extraction parameters were as follows: extraction temperature, 61.4 °C, extraction time, 78.7 min, ethanol volume, 79.4 %, at a fixed ultrasonic power of 200 W. Under the modified optimal conditions, the model predicted a total chlorophyll content of 30.1 mg/g. Verification of the optimization showed that chlorophyll extraction of 31.1 ± 1.56 mg/g was observed under the optimal conditions, which well matches with the predicted value. Under these conditions, two stage extraction could sufficiently reach the maximal chlorophyll yield (35.2 mg/g), and the extraction rate reached up to 88.9 %. The present paper provides a feasible technology route for comprehensive utilization of bioactive substances from Chlorella and microalgal biomass biorefinery.

Keywords

Chlorophyll Chlorella vulgaris Ultrasound-assisted extraction Response surface methodology Optimization 

Notes

Acknowledgement

Financial support was provided by National Science Found for Distinguished Young Scholars of China (Grant No. 20625308) and Research Fund for Young Teachers of Northwest Normal University (Grant No. NWNU-LKQN-10-30).

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Copyright information

© Association of Food Scientists & Technologists (India) 2012

Authors and Affiliations

  • Weibao Kong
    • 1
    • 2
  • Na Liu
    • 1
  • Ji Zhang
    • 1
  • Qi Yang
    • 1
  • Shaofeng Hua
    • 2
  • Hao Song
    • 2
  • Chungu Xia
    • 2
  1. 1.College of Life ScienceNorthwest Normal UniversityLanzhouChina
  2. 2.State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhouChina

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