Journal of Food Science and Technology

, Volume 51, Issue 1, pp 148–152 | Cite as

Molecular assay to fraud identification of meat products

  • Abbas DoostiEmail author
  • Payam Ghasemi Dehkordi
  • Ebrahim Rahimi
Original Article


Detection of species fraud in meat products is important for consumer protection and food industries. A molecular technique such as PCR method for detection of beef, sheep, pork, chicken, donkey, and horse meats in food products was established. The purpose of this study was to identification of fraud and adulteration in industrial meat products by PCR-RFLP assay in Iran. In present study, 224 meat products include 68 sausages, 48 frankfurters, 55 hamburgers, 33 hams and 20 cold cut meats were collected from different companies and food markets in Iran. Genomic DNA was extracted and PCR was performed for gene amplification of meat species using specific oligonucleotid primers. Raw meat samples are served as the positive control. For differentiation between donkey’s and horse’s meat, the mitochondrial DNA segment (cytochrome-b gene) was amplified and products were digested with AluI restriction enzyme. Results showed that 6 of 68 fermented sausages (8.82%), 4 of 48 frankfurters (8.33%), 4 of 55 hamburgers (7.27%), 2 of 33 hams (6.6%), and 1 of 20 cold cut meat (5%) were found to contain Haram (unlawful or prohibited) meat. These results indicate that 7.58% of the total samples were not containing Halal (lawful or permitted) meat and have another meat. These findings showed that molecular methods such as PCR and PCR-RFLP are potentially reliable techniques for detection of meat type in meat products for Halal authentication.


PCR-RFLP Mitochondrial DNA Meat species Haram Halal Iran 



The authors would like to thank all the staff of Biotechnology Research Center of Islamic Azad University of Shahrekord Branch in southwest Iran for their sincere support.


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Copyright information

© Association of Food Scientists & Technologists (India) 2011

Authors and Affiliations

  • Abbas Doosti
    • 1
    Email author
  • Payam Ghasemi Dehkordi
    • 1
  • Ebrahim Rahimi
    • 2
  1. 1.Biotechnology Research CenterIslamic Azad UniversityShahrekordIran
  2. 2.Department of Food Hygiene, Faculty of Veterinary MedicineIslamic Azad UniversityShahrekordIran

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