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Journal of Food Science and Technology

, Volume 50, Issue 6, pp 1097–1105 | Cite as

Chemical composition and physical quality characteristics of Ghanaian cocoa beans as affected by pulp pre-conditioning and fermentation

  • Emmanuel Ohene AfoakwaEmail author
  • Jennifer Quao
  • Jemmy Takrama
  • Agnes Simpson Budu
  • Firibu Kwesi Saalia
Original Article

Abstract

Investigations were conducted to evaluate the effects of pod storage (as a means of pulp preconditioning) and fermentation on the chemical composition and physical characteristics of Ghanaian cocoa beans. A 4 × 2 full factorial design with factors as pod storage (0, 7, 14, 21 days) and cocoa treatment (fermented and unfermented) were conducted. Samples were analyzed for their chemical composition (moisture, crude fat, crude protein, ash and carbohydrate content) and mineral content using standard analytical methods. The physical qualities of the beans were analyzed for their proportions of cocoa nibs, shells and germ. Fermentation and increasing pod storage resulted in significant (P < 0.05) decreases in ash (3.48–2.92%), protein (21.63–17.62%) and fat (55.21–50.40%) content of the beans while carbohydrate content increased from 15.47% to 24.93% with both treatments. As well, increasing pod storage and fermentation significantly (P < 0.05) increased the copper content of the beans from while reductions in Mg and K occurred. Amongst the minerals studied, potassium was the most abundant mineral followed by magnesium, phosphorus and calcium in the fermented cocoa beans. Proportion of cocoa nibs also increased from with increasing pod storage and fermentation whiles reductions in shell content and no appreciable changes in germ proportions were noted.

Keywords

Theobroma cacao Pod storage Forastero Fermentation Chemical composition Physical quality 

Notes

Acknowledgment

The authors want to thank the Cocoa Research Institute of Ghana, Tafo, Eastern Region, Ghana for providing the cocoa pods used in research study.

References

  1. Afoakwa EO (2010) Chocolate science and technology. Wiley-Blackwell Publishers, Oxford, UK, pp 3–22CrossRefGoogle Scholar
  2. Afoakwa EO, Paterson A (2010) Cocoa fermentation: chocolate flavour quality. Encyclopedia of biotechnology in agriculture and food. Taylor & Francis Publishing Inc., Oxford, U.K, pp 457–468Google Scholar
  3. Afoakwa EO, Paterson A, Fowler M, Ryan A (2008) Flavour formation and character in cocoa and chocolate: a critical review. Crit Rev Food Sci Nutr 48:840–857CrossRefGoogle Scholar
  4. Afoakwa EO, Paterson A, Fowler M (2007) Factors influencing rheological and textural qualities in chocolate – a review. Trends Food Sci Technol 18:290–298CrossRefGoogle Scholar
  5. Adeyeye EI, Akinyeye RO, Ogunlade I, Olaofe O, Boluwade JO (2010) Effect of farm and industrial processing on the amino acid profile of cocoa beans. Food Chem 118:357–363CrossRefGoogle Scholar
  6. Aremu CY, Agiang MA, Ayatse JOI (1995) Nutrient and anti-nutrient profiles of raw and fermented cocoa beans. Plant Foods Human Nutr 48:221–223Google Scholar
  7. Asiedu JJ (1989) Processing tropical crops: a technological approach. Macmillan Press Limited, London, pp 24–41Google Scholar
  8. AOAC (2005) Official methods of analysis, 18th edn. Association of Official Analytical Chemists, Washington, D.C USAGoogle Scholar
  9. Biehl B, Meyer B, Crone G, Pollmann L, Said MB (1989) Chemical and physical changes in the pulp during ripening and post-harvest storage of cocoa pods. J Sci Food Agric 48:189–208CrossRefGoogle Scholar
  10. Biehl B, Brunner E, Passern D, Quesnel VC, Adomako D (1985) Acidification, proteolysis and flavour potential in fermenting cocoa beans. J Sci Food Agric 36:583–598CrossRefGoogle Scholar
  11. Biehl B, Passern D (1982) Proteolysis during fermentation-like incubation of cocoa seeds. J Sci Food Agric 33:1280–1290CrossRefGoogle Scholar
  12. Crouzillat D, Lerceteau E, Rogers J, Petiard V (1999) Evolution of cacao bean proteins during fermentation: a study by two-dimensional electrophoresis. J Sci Food Agric 79:619–625CrossRefGoogle Scholar
  13. Dand R (1997) The international Cocoa trade. John Wiley & Sons, Inc, New York USA, p 102Google Scholar
  14. Duncan RJE (1984) A survey of Ghanaian cocoa farmers’ fermentation and drying practices and their implications for Malaysian practices. Proceedings of International Conference on Cocoa and Coconut, 15–17 October, 1984, Kuala Lumpur, MalaysiaGoogle Scholar
  15. Frost DJ, Adhikari K, Lewis DS (2011) Effect of barley flour on the physical and sensory characteristics of chocolate chip cookies. J Food Sci Technol. doi: 10.1007/s13197-010-0179-x
  16. Fowler MS (2009) Cocoa Beans: From Tree to Factory. In: Beckett ST (ed) Industrial chocolate manufacture and use, 4th edn. Wiley-Blackwell Science, Oxford, UK, pp 137–152Google Scholar
  17. Gotsch N (1997) Cocoa biotechnology: status, constraints and future prospects. Biotech Adv 15:333–352CrossRefGoogle Scholar
  18. ICCO (2009) ICCO document: Annual report 2007/2008. International Cocoa Organization, London U.KGoogle Scholar
  19. Jayathilakan K, Sultana K, Radhakrishna K, Bawa AS (2011) Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review. J Food Sci Technol. doi: 10.1007/s13197-011-0290-7
  20. Knudsen MH, Fold N (2011) Land distribution and acquisition practices in Ghana’s cocoa frontier: The impact of a state-regulated marketing system. Land Use Pol 28:378–387CrossRefGoogle Scholar
  21. Kratzer U, Frank R, Kalbacher H, Biehl B, Wöstemeyer J, Voigt J (2009) Subunit structure of the vicilin-like globular storage protein of cocoa seeds and the origin of cocoa- and chocolate-specific aroma precursors. Food Chem 113:903–913CrossRefGoogle Scholar
  22. Lehrian DW, Patterson GR (1983) Cocoa fermentation. In: Reed G (ed) Biotechnology, a comprehensive treatise, vol. 5. Verlag Chemie, Basel, pp 529–575Google Scholar
  23. Mamot S, Samarakhody RJ (1984) Cocoa fermentation effect of surface area, frequency of turning and depth of cocoa masses. In: International Conference on Cocoa and Coconut, 15–17 October, 1984, Kuala Lumpur, Malaysia.Google Scholar
  24. Meyer B, Biehl B, Said M, Samarakoddy RJ (1989) Postharvest pod storage: a method for pulp preconditioning to impair strong nib acidification during cocoa fermentation in Malaysia. J Sci Food Agric 48:285–304CrossRefGoogle Scholar
  25. Nout MJR, Motarjemi Y (1997) Assessment of fermentation as a household technology for improving food safety: a joint FAO/WHO workshop. Food Cont 8:221–226CrossRefGoogle Scholar
  26. Ntiamoah A, Afrane G (2008) Environmental impacts of cocoa production and processing in Ghana: life cycle assessment approach. J Cleaner Prod 16:1735–1740CrossRefGoogle Scholar
  27. Ostovar K, Keeney PG (1973) Isolation and characterization of microorganisms involved in the fermentation of Trinidad’s cacao beans. J Food Sci 38:611–617CrossRefGoogle Scholar
  28. Ouattara HG, Koffi BL, Karou GT, Sangare A, Niamke SL, Diopoh JK (2008) Implication of Bacillus spp. in the production of pectinolytic enzymes during cocoa fermentation. World J Microbiol Biotechnol 24:1753–1760CrossRefGoogle Scholar
  29. Pandey A, Singh G (2011) Development and storage study of reduced sugar soy containing compound chocolate. J Food Sci Technol 48:76–82CrossRefGoogle Scholar
  30. Páramo D, García-Alamilla P, Salgado-Cervantes MA, Robles-Olvera VJ, Rodríguez-Jimenes GC, García-Alvarado MA (2010) Mass transfer of water and volatile fatty acids in cocoa beans during drying. J Food Eng 99:276–283CrossRefGoogle Scholar
  31. Reineccius GA, Andersen DA, Kavanagh TE, Keeney PG (1972) Identification and quantification of the free sugars in cocoa beans. J Agric Food Chem 20:199–202CrossRefGoogle Scholar
  32. Rodriguez-Campos J, Escalona-Buendía HB, Orozco-Avila I, Lugo-Cervantes E, Jaramillo-Flores ME (2011) Dynamics of volatile and non-volatile compounds in cocoa (Theobroma cacao L.) during fermentation and drying processes using principal components analysis. Food Res Int 44:250–258CrossRefGoogle Scholar
  33. Rohan TA (1963). Processing of raw cocoa for the market. FA0 Technical Bulletin No 60. FAO, ItalyGoogle Scholar
  34. Rossini K, Noreña CPZ, Brandelli A (2011) Changes in the color of white chocolate during storage: potential roles of lipid oxidation and non-enzymatic browning reactions. J Food Sci Technol 48:305–311CrossRefGoogle Scholar
  35. Sanchez J, Daquenet G, Guiraud JP, Vincent JC, Galzy P (1985) A study of the yeast flora and the effect of pure culture seedling during the fermentation process of cocoa beans. Lebensm - Wiss Technol l8:69–75Google Scholar
  36. Schumacher AB, Brandelli A, Macedo FC, Pieta L, Klug TV et al (2010) Chemical and sensory evaluation of dark chocolate with addition of quinoa (Chenopodium quinoa Willd.). J Food Sci Technol 47:202–206CrossRefGoogle Scholar
  37. Schwan RF, Wheals AE (2004) The microbiology of cocoa fermentation and its role in chocolate quality. Crit Rev Food Sci Nutr 44:205–221CrossRefGoogle Scholar
  38. Schwan RF, Rose AH, Board RG (1995) Microbial fermentation of cocoa beans, with emphasis on enzymatic degradation of the pulp. J Applied Bacteriol 79:96S–107SGoogle Scholar
  39. Svanberg U, Lorri W (1997) Fermentation and nutrient availability. Food Cont 8:319–327CrossRefGoogle Scholar
  40. Thompson SS, Miller KB, Lopez AS (2007) Cocoa and coffee. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food microbiology fundamentals and frontiers. ASM Press, Washington, DC, pp 837–850Google Scholar
  41. White A, Handler P, Smith EL, Hill RL, Lehman IR (1978) Carbohydrate metabolism I. In: White L et al (eds) Principles of biochemistry, 6th edn. McGraw Hill, New York, pp 423–476Google Scholar
  42. Wood GAR, Lass RA (1985) Cocoa, 4th edn. Longman Group, London, U.KGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2011

Authors and Affiliations

  • Emmanuel Ohene Afoakwa
    • 1
    Email author
  • Jennifer Quao
    • 1
  • Jemmy Takrama
    • 2
  • Agnes Simpson Budu
    • 1
  • Firibu Kwesi Saalia
    • 1
  1. 1.Department of Nutrition & Food ScienceUniversity of GhanaAccraGhana
  2. 2.Cocoa Research Institute of GhanaNew TafoGhana

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