Abstract
Lactobacillus brevis FPTLB3 was isolated from freshwater fish, capable of producing bacteriocin that had broad spectrum of inhibition (3200 AU/ml) against Escherichia coli MTCC 1563, Enterococcus faecalis MTCC 2729, Lactobacillus casei MTCC 1423, Lactobacillus sakei ATCC 15521 and Staphylococcus aureus ATCC 25923. The antimicrobial activity of crude supernatant fluid was stable after heating at 121 °C for 60 min and declined thereafter. Stability of antimicrobial activity was observed at pH range of 2.0 to 8.0. Its active principle was proteinaceous in nature since the bacteriocin was inactivated by proteolytic enzymes, but not by other non–proteolytic enzymes. Mitomycin C and UV light did not affect the activity of the bacteriocin, while chloroform extraction completely destroyed their activity. Exposure to surfactant resulted in an increase in titre, except Nonidet P-40, which led to total loss of activity. No bacteriocin adsorption was detected at pH 1 to 2, whereas 100% bacteriocin adsorption was found at pH 6.5. Based on Tricine SDS-PAGE the estimated molecular mass of bacteriocin was 54 kDa. No plasmid was found to present in the isolate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Bar N, Harris ND, Rill RL (1987) Purification and properties of an antimicrobial substance produced by Lactobacillus bulgaricus. J Food Sci 52:411–415
Banach S, Rehberger1 T, Parrott1 T, Maxwell C, Coalson J, Touchette K (2002) Influence of Lactobacillus brevis Strain 1E-1 on the gastrointestinal microflora of pre-weaning and weaning pigs. Presented at the 2002 American Society of Animal Science Meeting
Bhattacharyya BK, Bhattacharjee D (2007) Bacteriocin: a biological food preservative. J Food Sci Technol 44(5):459–464
Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulphate-polyacrilamide gel electrophoresis. J Ind Microbiol 2:319–322
Borpuzari RN, Borpuzari T, Deuri B (2007) Probiotic characteristics of Lactobacillus acidophilus strains isolated from fermented milk. J Food Sci Technol 44(2):158–160
Caplice E, Fitzgerald GE (1999) Food fermentations: role of microorganisma in food production and preservation. Int J Food Microbiol 50:131–149
Chin HS, Chin JS, Kim JM, Yang R, Yoon SS (2001) Detection and antibacterial activity of a bacteriocin produced by Lactobacillus plantarum. Food Sci Biotechnol 10:335–341
Corsetti A, Settanni L, Van Sinderen D (2004) Characterization of bacteriocin-like inhibitory substances (BLIS) from Sourdough lactic acid acid bacteria and evaluation of their in vitro and in situ activity. J Appl Microbiol 96:521–534
Graciella M, Vignolo M, de Kairuz N, Aida AP, de Ruiz H, Oilver G (1995) Influence of growth conditions on the production of lacticin 705, a bacteriocin produced by Lactobacillucasei CRL 705. J Appl Bacteriol 78:5–10
Hazarika RA, Singh DK, Kapoor KN, Malik SVS, Agarwal RK (2007) Effect of nisin-producing lactic acid bacteria on the survivability of verotoxic Escherichia coli (VTEC) O111 in red meat gravy. J Food Sci Technol 44(5):524–526
Hernandez D, Cardell E, Zarate V (2005) Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillu plantarum TF711. J Appl Microbiol 99:77–84
Huot E, Maghrous J, Barena-Gonzalez C (1996) Bacteriocin J46, a new bacteriocin produced by Lactococcus lactis subsp. cremoris J46: Isolation and characterization of the protein and its gene. Anaerobe 2:137–145
Ivanova I, Miteva V, Ts S, Pantev A, Budakov I, Danova S, Moncheva P, Nikolova I, Dousset X, Boyaval P (1998) Characterization of a bacteriocin produced by Streptococcus thermophilus 81. Int J Food Microbiol 42:147–158
Jamuna M, Jeevaratnam K (2009) Antibacterial efficacy of nisin and pediocins against food spoilage and pathogenic organisms in broth and a vegetarian food. J Food Sci Technol 46(6):563–568
Kim SH, Shin BH, Kim YH, Nam SW, Jeon SJ (2007) Cloning and expression of a full-length glutamate decarboxylase gene from Lactobacillus brevis BH2. Biotechnol Bioprocess Eng 12:707–712
Kishi A, Uno K, Matsubara Y (1996) Effect of the oral administration of Lactobacillus brevis subsp. coagulans on interferon-alpha producing capacity in humans. J Am Coll Nutr 15:408–412
Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12:39–86
Leroy F, De Vuyst L (2002) Bacteriocin produced by Enterococcus faecium RZS C5 is cell density limited and occurs in the very early growth phase. Int J Food Microbiol 72:155–164
Messi P, Guerrieri E, Bondi M (2003) Bacteriocin-like substance (BLS) production in Aeromonas hydrophila water isolates. FEMS Microbiol Lett 220:121–125
Ogunbanwo ST, Sanni AI, Onilude AA (2003) Influence of cultureal conditions on the production of bacteriocin by Lactobacillus brevis OG1. African J Biotechnol 2(7):179–184
Rattanachaikunsopon P, Phumkhachorn P (2006) Isolation and preliminary characterization of bacteriocin produced by Lactobacillus plantarum N014 isolated from Nham, a traditional Thai fermented pork. J Food Prot 69(8):1937–1943
Schagger H, Von Jagow G (1987) Tricine sodium dodecyl sulphate polyacrilamide gel electrophoresis for the separation of proteins in the range of 1 to 100 kDa. Anal Biochem 166:368–379
Skytta E, Haikara A, Mattila-Sandholm T (1993) Production and characterization of antibacterial compounds produced by Pediococcus damnosus and P. pentosaceus. J Appl Bacteriol 74:134–142
Sudeepa ES, Tamil Selvi A, Bhaskar N (2007) Antimicrobial activity of a lipolytic and hydrocarbon degrading lactic acid bacterium isolated from fish processing waste. J Food Sci Technol 44(4):417–442
Todorov SD, Dicks LMT (2004) Partial characterization of bacteriocins produced by four lactic acid bacteria isolated from regional South African barley beer. Ann Microbiol 54(4):403–413
Todorov SD, Dicks LMT (2005) Pediocin ST18, an antilisterial bacteriocin produced by Pediococcus pentosaseus ST 18 isolated from boza, a tradition cereal beverage from Bulgaria. Process Biochem 40:365–370
Todorov SD, Dicks LMT (2006) Screening for bacteriocin-producing lactic acid bacteria from boza, a traditional cereal beverage from Bulgaria comparison of the bacteriocins. Process Biochem 41:11–19
Todorov S, Onno B, Sorokin O, Chobert JM, Ivanova I, Dousset X (1999) Detection and characterization of a novel antibacterial substance produced by Lactobacillus plantarum ST31 isolated from sourdough. Int J Food Microbiol 48:167–177
Villani F, Pepe O, Mouriello G, Salzano G, Moschetti G, Coppola S (1995) Antilisterial activity of thermophilin 347, a bacteriocin produced by Streptococcus thermophilus. Int J Food Microbiol 25:179–190
Wanda J, Bonita A (1991) Partial purification and characterization of a bacteriocin produced by Propionibacterium thoenii. Appl Env Microbiol 57:701–706
Yakabe Y, Moore EL, Yokota S, Sui H, Nobuta Y, Fukao M, Palmer H, Yajima N (2009) Safety assessment of Lactobacillus brevis KB290 as a probiotic strain. Food and Chemical Toxicology, FCT 4990, 15th July 2009 disk used, Model 5 G, No. of pages 4
Yang R, Johnson MC, Ray B (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Env Microbiol 58:3355–3359
Acknowledgement
The support from CFTRI (Mysore, India) and IMTECH (Chandigarh, India) is gratefully acknowledged for enriching this study with support of literature and providing microbial cultures respectively.
Author information
Authors and Affiliations
Corresponding author
Additional information
A part of M. F. Sc thesis of first author
Rights and permissions
About this article
Cite this article
Banerjee, S.P., Dora, K.C. & Chowdhury, S. Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish. J Food Sci Technol 50, 17–25 (2013). https://doi.org/10.1007/s13197-011-0240-4
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-011-0240-4