Effect of diets on digestive enzymes from worker termites of Odontotermes brunneus (Termitidae)
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The digestive system of termites may have several biotechnological applications. The enzyme activity of α-amylase, cellulase and protease in Odontotermes brunneus workers were tested against three different diets. Additionally, the effects of temperature (30–80 °C) and pH (2.0–10.0) on enzyme activities were evaluated. All enzymes investigated were detected in the gut extracts of worker termites. Odontotermes brunneus worker gut α-amylase and cellulase activity was high in wood diet 25 U and 1700 mU/mg and the protease activity was high in filter paper 130 U/mg. α-amylase in termites consisted of 15–25 U/mg protein when the pH was about 4.0–6.0. Cellulase in termites consisted of 1000 U/mg protein when the pH was about 4.0–6.0. Protease in termites consisted of 125 U/mg protein when the pH was about 6.0–8.0. Also the enzyme activity on temperature was influenced by different diet in O. brunneus. Over all study revealed that the diet of O. brunneus from different source may influence the enzyme production and its stability, which can be used as individual resources. The influence of mixed diet and the involvement of enzymes producing micro bacteria need to be further analyzed.
KeywordsWorker Enzymes Odontotermes brunneus Protease Amylase Cellulase
The authors thank our department of PG and Research Centre in Biotechnology for providing infrastructure facility and we thank Dr. P. Balaji Head of the department for his support to carry out this work.
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Conflict of interest
The authors declared that we have no conflict of interest.
- Ahmed S, Mustafa T, Riaz MA, Hussain A (2006) Efficacy of insecticides against subterranean termites in sugarcane. Int J Agric Biol 8:508–510Google Scholar
- Campbell NA, Reece JB, Urry LA, Cain ML, Wasserman SA, Minorsky PV, Jackson RB (2008) Biology, Eight edn. Pearson-Benjamin Cummings, LondonGoogle Scholar
- Chottani OB (1997) Fauna of India–isoptera (termites) Vol. II, pp. xx + 801 (Published Director.ZSI. Calcutta)Google Scholar
- Drapcho CM, Nhuan NP, Walker TH (2008) Biofuels engineering process technology. The McGraw-Hill companies and Industries, New YorkGoogle Scholar
- Fagbohunka BS, Edorh SE, Adeyanju MM, Ezima EN, Alabi MA, Ogunlabi OO (2015) Activites of a cellulase of the termite, Ametermes eveuncifer (Silverstri) soldier: clue to termites salt intolerance. J Nat Sci Res 5(11):117–121Google Scholar
- Haddar A, Agrebi R, Bougatef A, Hmidet N, Sellami-Kamoun A, Nasri M (2009) Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: purification, characterization and potential application as a laundry detergent additive. Biores Technol 100:3366–3373. https://doi.org/10.1016/j.biortech.2009.01.061 CrossRefGoogle Scholar
- Hmidet N, Ali NE, Haddar A, Kanoun S, Alya S, Nasri MJ (2009) Alkaline proteases and thermo stable α-amylase co-produced by Bacillus licheniformis NH1: characterization and potential application as detergent additive. Biochem Eng 47:71–79. https://doi.org/10.1016/j.bej.2009.07.005 CrossRefGoogle Scholar
- Li X, Yang H, Roy B, Wang D, Yue W, Jiang L, Park EY, Miao Y (2009) The most stirring technology in future: cellulase enzyme and biomass utilization. Afr J Biotechnol 8(11):2418–2422Google Scholar
- Noirot C (1992) From wood- to humus feeding: an important trend in termite evolution. Biol Evol Soc Insects, pp 107–119Google Scholar
- Waller DA, La Fage JP (1986) Nutritional ecology of termites. In: Slansky F Jr, Rodriguez JG (eds) Nutritional ecology of insects, mites, spiders, and related invertebrates. Wiley, New York, pp 487–532Google Scholar
- Whistler RL, Chen CC (1991) Hemicelluloses. In: Lewin M, Goldstein IS (eds) Wood structure and composition. International fiber science and technology series, vol 11. Marcel Decker Inc, New York, pp 287–320Google Scholar