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mRNA Expression Analysis of E-Cadherin, VEGF, and MMPs in Gastric Cancer: a Pilot Study

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Abstract

Gastric cancer (GC) is a serious fatal cancer on a global scale because of its presentation at advanced stage. The expressions of vascular endothelial growth factor (VEGF), E-cadherin, and matrix metalloproteinases (MMPs) in other cancers have been reported. However, its expression and underlying mechanisms are little known in gastric cancer in Indian context. In this study, we detected mRNA expression of VEGF, E-cadherin, and MMPs (MMP-1, MMP-2, and MMP-9) in 73 gastric cancer tissues and 27 normal controls by reverse-transcriptase polymerase chain reaction (RT-PCR). Receiver operator characteristics analysis was done for determining the diagnostic utility of VEGF, MMPs and E-cadherin with respect to the sensitivity and specificity. The association of VEGF, MMPs, and E-cadherin expression with the clinicopathological characteristics and the prognosis was subsequently analyzed. The mRNA expression results showed that E-cadherin was significantly downregulated in 47.9% of GC in comparison to control. There was no change in VEGF expression observed in 90.4% GC cases. MMP-1, MMP-2, and MMP-9 were overexpressed in 13.7%, 28.8%, and 11% of GC, respectively, with significant change in MMP-2 (p ≤ 0.0001) and MMP-9 (p = 0.027) in comparison to control. Our results strengthen the necessity of more studies to elucidate the prophetic role of these genes in the development of gastric cancer.

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Authors and Affiliations

  1. Department of Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Puneet Kumar, Arun Sebastian, Khushi Verma, Ruhi Dixit, Soni Kumari & Satyendra Kumar Tiwary

  2. Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

    Soni Kumari & Gopeshwar Narayan

  3. Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Juhi Singh

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  1. Puneet Kumar
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All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki Declaration ethical standards and approved by the Institutional Ethical Committee of Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. The study was approved by the Institutional Ethical Committee of Institute of Medical Sciences, Banaras Hindu University.

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Kumar, P., Sebastian, A., Verma, K. et al. mRNA Expression Analysis of E-Cadherin, VEGF, and MMPs in Gastric Cancer: a Pilot Study. Indian J Surg Oncol 12 (Suppl 1), 85–92 (2021). https://doi.org/10.1007/s13193-020-01096-5

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