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Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation

  • Original Article
  • Published:
The Journal of Indian Prosthodontic Society

Abstract

The aims of this study were to evaluate the effectiveness of six disinfection methods and the influence of these methods on the adaptation of maxillary dentures. Acrylic resin specimens contaminated with fungi were exposed to the following disinfection treatments: 1. microwave oven (900 W) at full power for 5 min (with soaking the specimen in 250 ml water), 2. microwave oven at medium power for 5 min (with soaking the specimen in 250 ml water), 3. sodium hypochlorite 5.25 % for 5 min, 4. diluted sodium hypochlorite 1:420 for 5 h, 5. Chlorhexidine gluconate for 5 h, 6. effervescent tablets for 15 min, 7. soaking in 250 ml tap water for 15 min. Colony forming units (CFUs) of remaining cells were counted and compared with t test (p ≤ 0.05). Dimensional stability was evaluated using aluminum die simulating the maxillary edentulous arch. Posterior palatal gaps were measured. Data were analyzed using one-way ANOVA test and t test (p ≤ 0.05). Microwave irradiation (at full or medium power) and sodium hypochlorite 5.25 % for 5 min were able to reduce the CFUs of fungi by more than 4 log10 whereas diluted sodium hypochlorite, chlorhexidine gluconate, and effervescent tablets did not achieve a reduction of >2.8, 2.68 and 1.66, respectively. For dimensional stability test, t test revealed significant difference between control group and the microwave at full power group (p = 0.000). Within the limits of this study, microwave oven at medium power and sodium hypochlorite (5.25 %) are effective and safe methods of disinfecting removable dentures.

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References

  1. Jeganathan S, Lin C (1992) Denture stomatitis: a review of the aetiology, diagnosis and management. Aust Dent J 37:107–114

    Article  PubMed  Google Scholar 

  2. McCourtie J, Douglas L (1985) Extracellular polymer of Candida albicans: isolation, analysis and role in adhesion. J Gen Microbiol 131:495–503

    PubMed  Google Scholar 

  3. Theilade E, Budtz-Jorgensen E (1988) Predominant cultivable microflora of plaque on removable dentures in patients with denture induced stomatitis. Oral Microbiol Immunol 3:8–13

    Article  PubMed  Google Scholar 

  4. Kulak Y, Arikan A, Kazazoglu E (1997) Existence of Candida albicans and microorganisms in denture stomatitis patients. J Oral Rehabil 24:788–790

    Article  PubMed  Google Scholar 

  5. Machado A, Breeding L, Vergani C, da Cruz Perez L (2009) Hardness and surface roughness of reline and denture base acrylic resins after repeated disinfection procedures. J Prosthet Dent 102:115–122

    Article  PubMed  Google Scholar 

  6. Rohrer M, Bulard R (1985) Microwave sterilization. J Am Dent Assoc 110:194–198

    Article  PubMed  Google Scholar 

  7. Silva M, Consani R, Sardi J, Mesquita M, Macêdo A, Takahashi J (2013) Microwave irradiation as an alternative method for disinfection of denture base acrylic resins. Minerva Stomatol 62:23–29

    PubMed  Google Scholar 

  8. Altieri K, Sanitá P, Machado A, Giampaolo E, Pavarina A, Vergani C (2012) Effectiveness of two disinfectant solutions and microwave irradiation in disinfecting complete dentures contaminated with methicillin-resistant Staphylococcus aureus. J Am Dent Assoc 143:270–277

    Article  PubMed  Google Scholar 

  9. Dovigo L, Pavarina A, Ribeiro D, de Oliveira J, Vergani C, Machado A (2009) Microwave disinfection of complete dentures contaminated in vitro with selected bacteria. J Prosthodont 18:611–617

    Article  PubMed  Google Scholar 

  10. Ribeiro D, Pavarina A, Dovigo L, Palomari Spolidorio D, Giampaolo E, Vergani C (2009) Denture disinfection by microwave irradiation: a randomized clinical study. J Dent 37:666–672

    Article  PubMed  Google Scholar 

  11. Dixon D, Breeding L, Faler T (1999) Microwave disinfection of denture base materials colonized with Candida albicans. J Prosthet Dent 81:207–214

    Article  PubMed  Google Scholar 

  12. Silva M, Mima E, Colombo A, Sanitá P, Jorge J, Massucato E, Vergani C (2012) Comparison of denture microwave disinfection and conventional antifungal therapy in the treatment of denture stomatitis: a randomized clinical study. Oral Surg Oral Med Oral Pathol Oral Radiol 114:469–479

    Article  PubMed  Google Scholar 

  13. Sanita P, Machado A, Pavarina A, Massucato E, Colombo A, Vergani C (2012) Microwave denture disinfection versus nystatin in treating patients with well-controlled type 2 diabetes and denture stomatitis: a randomized clinical trial. Int J Prosthodont 25:232–244

    PubMed  Google Scholar 

  14. Nirale RM, Thombre R, Kubasad G (2012) Comparative evaluation of sodium hypochlorite and microwave disinfection on dimensional stability of denture bases. J Adv Prosthodont 4:24–29

    Article  PubMed Central  PubMed  Google Scholar 

  15. Senna P, Da Silva W, Faot F, Del Bel Cury A (2011) Microwave disinfection: cumulative effect of different power levels on physical properties of denture base resins. J Prosthodont 20:606–612

    Article  PubMed  Google Scholar 

  16. Basso M, Giampaolo E, Vergani C, Machado A, Pavarina A, Compagnoni M (2010) Influence of microwave disinfection on the linear dimensional stability of complete dentures: a clinical study. Int J Prosthodont 23:318–320

    PubMed  Google Scholar 

  17. Consani R, Iwasaki R, Mesquita M, Mendes W, Consani S (2008) Effect of repeated simulated disinfections by microwave energy on the complete denture base adaptation. Open Dent J 2:61–66

    Article  PubMed Central  PubMed  Google Scholar 

  18. Seo R, Vergani C, Pavarina A, Compagnoni M, Machado A (2007) Influence of microwave disinfection on the dimensional stability of intact and relined acrylic resin denture bases. J Prosthet Dent 98:216–223

    Article  PubMed  Google Scholar 

  19. Fleck G, Ferneda F, da Ferreira Silva DF, Mota EG, Shinkai RS (2007) Effect of two microwave disinfection protocols on adaptation of poly (methyl methacrylate) denture bases. Minerva Stomatol 56:121–127

    PubMed  Google Scholar 

  20. Sartori E, Schmidt C, Walber L, Shinkai R (2006) Effect of microwave disinfection on denture base adaptation and resin surface roughness. Braz Dent J 17:195–200

    Article  PubMed  Google Scholar 

  21. Pavan S, Arioli Filho JN, Dos Santos PH, Mollo Fde A Jr (2005) Effect of microwave treatments on dimensional accuracy of maxillary acrylic resin denture base. Braz Dent J 16:119–123

    Article  PubMed  Google Scholar 

  22. Basso M, Giampaolo E, Machado A, Pavarina A, Vergani C (2012) Evaluation of the occlusion vertical dimension of complete dentures after microwave disinfection. Gerodontology 29:e815–e821

    Article  PubMed  Google Scholar 

  23. Furukawa K, Niagro F, Runyan D, Cameron S (1998) Effectiveness of chlorine dioxide in disinfection on two soft denture liners. J Prosthet Dent 80:723–729

    Article  PubMed  Google Scholar 

  24. Silva M, Vergani C, Giampaolo E, Neppelenbroek K, Spolidorio D, Machado A (2006) Effectiveness of microwave irradiation on the disinfection of complete dentures. Int J Prosthodont 19:288–293

    PubMed  Google Scholar 

  25. Sanitá P, Vergani C, Giampaolo E, Pavarina A, Machado A (2009) Growth of Candida species on complete dentures: effect of microwave disinfection. Mycoses 52:154–160

    Article  PubMed  Google Scholar 

  26. Rudd R, Senia E, McCleskey F, Adams ED (1984) Sterilization of complete dentures with sodium hypochlorite. J Prosthet Dent 51:318–321

    Article  PubMed  Google Scholar 

  27. Baysan A, Whiley R, Wright PS (1998) Use of microwave energy to disinfect a long-term soft lining material contaminated with Candida albicans or Staphylococcus aureus. J Prosthet Dent 79:454–458

    Article  PubMed  Google Scholar 

  28. Tamamoto M, Hamada T, Miyake Y, Suginaka H (1985) Ability of enzymes to remove Candida. J Prosthet Dent 53:214–216

    Article  PubMed  Google Scholar 

  29. Lal K, Santarpia RP 3rd, Pollock J, Renner R (1992) Assessment of antimicrobial treatment of denture stomatitis using an in vivo replica model system: therapeutic efficacy of an oral rinse. J Prosthet Dent 67:72–77

    Article  PubMed  Google Scholar 

  30. Burns D, Kazanoglu A, Moon P, Gunsolley J (1990) Dimensional stability of acrylic resin materials after microwave sterilization. Int J Prosthodont 3:489–493

    PubMed  Google Scholar 

  31. Polyzois G, Zissis A, Yannikakis S (1995) The effect of glutaraldehyde and microwave disinfection on some properties of acrylic denture resin. Int J Prosthodont 8:150–154

    PubMed  Google Scholar 

  32. Neppelenbroek K, Pavarina A, Spolidorio D, Vergani C, Mima E, Machado A (2003) Effectiveness of microwave sterilization on three hard chairside reline resins. Int J Prosthodont 16:616–620

    PubMed  Google Scholar 

  33. Craig R (1997) Prosthetic applications of polymers in restorative dental materials, 12th edn. Mosby, St. Louis, pp 513–547

    Google Scholar 

  34. Thomas C, Webb B (1995) Microwaving of acrylic resin dentures. Eur J Prosthodont Restor Dent 3:179–182

    PubMed  Google Scholar 

  35. Brondani MA, Samim F, Feng H (2012) A conventional microwave oven for denture cleaning: a critical review. Gerodontology 29:e6–e15

    Article  PubMed  Google Scholar 

  36. Senna P, da Silva W, Cury A (2012) Denture disinfection by microwave energy: influence of Candida albicans biofilm. Gerodontology 29:e186–e191

    Article  PubMed  Google Scholar 

  37. Senna P, Sotto-Maior B, Silva W, Del Bel Cury A (2013) Adding denture cleanser to microwave disinfection regimen to reduce the irradiation time and the exposure of dentures to high temperatures. Gerodontology 30:26–31

    Article  PubMed  Google Scholar 

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Acknowledgments

The author would like to thank Assoc. Prof. Abir Al-Kafri, Faculty of Medicine, for her assistance in disinfection test and Prof. Dr. Rafi Jabra, Higher Institution for Applied Science and Technology, for his help in dimensional stability test. Special acknowledgment is directed to Damascus University for the financial support of this study.

Conflict of interest

The author has no known conflicts of interest associated with the products used in this study and there has been no financial support for this work that could have influenced its outcome.

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Authors and Affiliations

  1. Department of Removable Prosthodontics, Faculty of Dentistry, Damascus University, P.O. Box 4926, Damascus, Syria

    Mohannad H. Al-Saadi

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  1. Mohannad H. Al-Saadi
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Correspondence to Mohannad H. Al-Saadi.

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Al-Saadi, M.H. Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation. J Indian Prosthodont Soc 14 (Suppl 1), 24–30 (2014). https://doi.org/10.1007/s13191-014-0354-2

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  • DOI: https://doi.org/10.1007/s13191-014-0354-2

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