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Effect of probiotics on obesity-related markers per enterotype: a double-blind, placebo-controlled, randomized clinical trial

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Abstract

Background

Prevention and improvement of disease symptoms are important issues, and probiotics are suggested as a good treatment for controlling the obesity. Human gut microbiota has different community structures. Because gut microbial composition is assumed to be linked to probiotic function, this study evaluated the efficacy of probiotics on obesity-related clinical markers according to gut microbial enterotype.

Methods

Fifty subjects with body mass index over 25 kg/m2 were randomly assigned to either the probiotic or placebo group. Each group received either unlabeled placebo or probiotic capsules for 12 weeks. Body weight, waist circumference, and body composition were measured every 3 weeks. Using computed tomography, total abdominal fat area and visceral fat area were measured. Blood and fecal samples were collected before and after the intervention for biochemical parameters and gut microbial compositions analysis.

Results

Gut microbial compositions of all the subjects were classified into two enterotypes according to Prevotella/Bacteroides ratio. The fat percentage, blood glucose, and insulin significantly increased in the Prevotella-rich enterotype of the placebo group. The obesity-related markers, such as waist circumference, total fat area, visceral fat, and ratio of visceral to subcutaneous fat area, were significantly reduced in the probiotic group. The decrease of obesity-related markers was greater in the Prevotella-rich enterotype than in the Bacteroides-rich enterotype.

Conclusion

Administration of probiotics improved obesity-related markers in obese people, and the efficacy of probiotics differed per gut microbial enterotype and greater responses were observed in the Prevotella-dominant enterotype.

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Funding

This research was supported by Main Research Program (E0170601-03) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science and ICT.

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  1. Eun-Ji Song and Kyungsun Han contributed equally to this work.

Authors and Affiliations

  1. Research Group of Healthcare, Korea Food Research Institute, Wanju-gun, 55365, Republic of Korea

    Eun-Ji Song & Young-Do Nam

  2. Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea

    Eun-Ji Song & Young-Do Nam

  3. Department of Rehabilitation Medicine of Korean Medicine, Dongguk University, Gyeonggi-do, 10326, Republic of Korea

    Kyungsun Han & Hojun Kim

  4. Clinical Medicine Division, Korea Institute of Oriental Medicine, Daejeon, 34054, Republic of Korea

    Kyungsun Han

  5. Research and Development Center, Cell Biotech Co. Ltd., Gimpo, 10003, Republic of Korea

    Tae-Joong Lim, Sanghyun Lim & Myung-Jun Chung

  6. Environmental Risk and Welfare Research Team, Korea Basic Science Institute, Seoul, 02841, Republic of Korea

    Myung Hee Nam

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Correspondence to Hojun Kim or Young-Do Nam.

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This study was conducted according to the principles of Declaration of Helsinki and good clinical practice guidelines. The study protocol was approved by the Institutional Review Board of Ilsan Dongguk University Hospital (approval number 2016-02) and registered in Clinical Research Information Service (CRIS identifier: KCT0002292). Written informed consent has also been obtained from all the participants.

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Song, EJ., Han, K., Lim, TJ. et al. Effect of probiotics on obesity-related markers per enterotype: a double-blind, placebo-controlled, randomized clinical trial. EPMA Journal 11, 31–51 (2020). https://doi.org/10.1007/s13167-020-00198-y

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