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Responses of Soil Active Organic Carbon Fractions and Enzyme Activities to Freeze-thaw Cycles in Wetlands

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Abstract

In order to clarify the response of soil active organic carbon fractions and enzyme activities in different type of wetlands to freeze-thaw cycles (FTCs), a FTCs simulation experiment of different type wetland soils were carried out. In the study, samples were collected from three soil layers of (0–10, 10–20 and 20–30 cm) undisturbed Deyeuxia purpurea wetland (UDPW), disturbed Deyeuxia purpurea wetland (DDPW) and rice paddy field (RP), and then exposed to FTCs at large (-10 to 10℃) or small (-5 to 5℃) amplitudes, respectively. The results showed that FTCs increased the soil dissolved organic carbon (DOC) concentration in the three soil layers of UDPW, DDPW and RP by approximately 4.7–45.1%, 3.8–41.9% and 1.1–32.7% at large amplitude, and 8.7–48.1%, 5.2–43.4% and 2.2–31.8% at small amplitude. The interaction between small amplitude and higher water content resulted in the maximum increment of DOC concentration. However, FTCs decreased microbial biomass carbon (MBC) concentration and cellulase, invertase and catalase activities, and particularly the interaction between the large amplitude and higher water content exerted the most significant effect. As the increase of freeze-thaw frequency, DOC concentrations increased firstly and then decreased, while MBC concentrations and the three enzyme activities were opposite to DOC. The average change in DOC and MBC concentrations and enzyme activities due to the effects of FTCs varied from soil type, and the variation of DOC, MBC and enzyme activities across different type wetlands were as follows: UDPW > DDPW > RP. As the soil depth increased, the FTCs effect gradually weakened, which was manifested as 0–10 cm > 10–20 cm > 20–30 cm. For the three wetland soils, the significant correlations between active organic carbon fractions and enzyme activities indicate that the increase in DOC due to FTCs plays an important role on soil microbes and enzyme activities. However, the correlation was weak in RP soil, which might be related to strong disturbance of human.

摘要

为了明确不同类型湿地土壤活性有机碳和土壤酶活性对冻融循环的响应, 本研究选取三江平原未受干扰的小叶章湿地, 受到干扰的小叶章湿地和水稻田 0–10, 10–20 和 20–30 cm 三个层的土壤进行冻融模拟实验, 实验分别设置大 (-10–10℃) 和小 (-5–5℃) 两个冻融幅度。实验结果显示, 冻融循环增加了未受干扰的小叶章湿地、受到干扰的小叶章湿地和水稻田三个土层的土壤可溶性有机碳浓度, 在大幅度冻融条件下, 可溶性有机碳浓度分别增加了4.7–45.1%, 3.8–41.9% 和 1.1–32.7%, 小幅度冻融条件下分别增加了8.7–48.1%,5.2–43.4% 和 2.2–31.8%。其中, 小振幅冻融循环和高含水量的交互作用对可溶性有机碳浓度影响最大。随着土壤活动层深度的增加, 冻融作用对三种土壤DOC浓度的影响均减弱。冻融循环却降低了微生物量碳浓度和纤维素酶、蔗糖酶和过氧化氢酶活性, 其中大幅度冻融和高含水量交互作用影响最为显著。随着冻融频率的增加, 可溶性有机碳浓度在初期增加后下降, 而微生物量碳浓度和三种酶活性却与可溶性有机碳浓度相反。冻融循环引起的土壤可溶性有机碳、微生物量碳浓度和酶活性的平均变化量在不同类型的湿地上存在差, 可溶性有机碳、微生物量碳和酶活性的变化: 未受干扰小叶章湿地 > 受干扰小叶章湿地 > 水稻田。随着土壤深度的增, 冻融循环效应逐渐减弱。总体上表现为; 0–10 cm > 10–20 cm > 20–30 cm。在3种湿地土壤, 活性有机碳组分与酶活性之间的显著相关性表明, 冻融循环增加的可溶性有机碳对土壤微生物和酶活性起着重要作用。而水稻田土壤的相关性较弱, 可能与人为干扰有关。

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

We gratefully acknowledge “National Natural Science Foundation of China” (No. 31670489); “Doctoral Fund project of Jiamusi University” (No. JMSUBZ2020-12) for financial support.

Funding

This work was supported by [National Natural Science Foundation of China] (Grant numbers. No. 31670489); and [Doctoral Fund project of Jiamusi University] (Grant numbers No. JMSUBZ2020-12).

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  1. The Faculty of Science of Jiamusi University, Jiamusi, 154007, China

    Jinqiu Guan, Chunxiang Song, Yude Wu, Xingtian Qi, Rongjun Qu & Fu Li

  2. Heilongjiang Academy of Forestry, Harbin, 150081, China

    Hongwei Ni

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  1. Jinqiu Guan
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Contributions

Jinqiu Guan, Fu Li and Hongwei Ni developed the idea of the study, participated in its design and coordination and helped to draft the manuscript. Chunxiang Song and Yude Wu contributed to the acquisition and interpretation of data. Xingtian Qi and Rongjun Qu provided critical review and substantially revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Fu Li or Hongwei Ni.

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Guan, J., Song, C., Wu, Y. et al. Responses of Soil Active Organic Carbon Fractions and Enzyme Activities to Freeze-thaw Cycles in Wetlands. Wetlands 42, 36 (2022). https://doi.org/10.1007/s13157-022-01553-7

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