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Heavy metal stress induced hyperglycemia in blue swimmer crab, Portunus pelagicus

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Abstract

The study was to find out the effect of cadmium and mercury on levels of hemolymph glucose, crustacean hyperglycemic hormone (CHH) and hepatopancreas glycogen in the blue swimmer crab Portunus pelagicus. The experiments were performed in both intact and eyestalk ablated crabs. Quantification of CHH was done by the indirect ELISA with the aid of primary anti-Carcinus maenas-CHH antibody. Higher glucose concentration was observed on exposure to 8×10–6 of cadmium ((825.6±5.42) μg/mL) and 6×10–6 of mercury ((90.5±6.25) μg/mL) after 48 h and 24 h respectively. Higher level of hemolymph glucose was observed in eyestalk intact crabs on exposure to cadmium and mercury than eyestalk ablated crabs. Decrease in the levels of CHH was observed in both eyestalk intact and ablated crabs on heavy metal exposure. Decline of the hepatopancreas glycogen level was also witnessed with the exposure to heavy metal, which validated its utilization in the production of glucose. Thus this study brings to light, the variations in hemolymph glucose, CHH and hepatopancreas glycogen on heavy metal stress. These carbohydrate metabolites can be used as biomarkers in assessing heavy metal contamination in water bodies.

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Acknowledgements

The authors thank H. Dircksen Stockholm University for kindly providing the CHH antiserum.

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Authors and Affiliations

  1. Department of Oceanography and Coastal Area Studies, Alagappa University, Thondi Campus, Thondi, 623409, Tamilnadu, India

    R. Saravanan, V. Sugumar & M. I. Beema Mahin

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  1. R. Saravanan
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  2. V. Sugumar
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  3. M. I. Beema Mahin
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Correspondence to V. Sugumar.

Additional information

Foundation item: The grant from the Department of Science and Technology, New Delhi under contract No. SR/FT/LS-137/2009 dt. 17.01.2012.

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Saravanan, R., Sugumar, V. & Beema Mahin, M.I. Heavy metal stress induced hyperglycemia in blue swimmer crab, Portunus pelagicus. Acta Oceanol. Sin. 37, 47–53 (2018). https://doi.org/10.1007/s13131-018-1211-7

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