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Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii

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Abstract

Phosphorus (P) is one of the key nutrients for the growth of phytoplankton. In this study, we used a method coupling label-free quantitation with liquid chromatography-mass spectrometry (LFQ-LC-MS/MS) to track the change of relative protein abundance between P-replete and P-deficient treatments in a non-model diatom, Thalassiosira weissflogii. Out of the 631 proteins identified, 132 were found to have significant changes in abundance (>1.5 folds) between the two treatments, especially those proteins involved in macromolecular biosynthesis pathways. For example, the up-regulation of sulfolipid biosynthesis protein in the P-deficient culture suggested a switch from using phospholipids to sulfolipids. In addition, the ribosome subunits and tRNA synthetases were down-regulated, which might explain the decrease in protein content in the P-deficient culture. A vacuolar sorting receptor homologous protein was found to be 9.2-folds up-regulated under P-deficiency, indicating an enhancement in the vacuolar sorting pathway for protein degradation. Our results show that T. weissflogii has sophisticated responses in multiple macromolecular metabolism pathways under P-deficiency, a mechanism which can be critical for this species to survive under various levels of P availability in the environment.

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, Xiamen University, Xiamen, 361005, China

    Xiuxiu Wang & Bangqin Huang

  2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China

    Xiuxiu Wang & Bangqin Huang

  3. Department of Marine Sciences, University of Connecticut, Groton, 06340, USA

    Huan Zhang

Authors
  1. Xiuxiu Wang
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  2. Bangqin Huang
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  3. Huan Zhang
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Correspondence to Bangqin Huang.

Additional information

Foundation item: The National Natural Science Foundation of China (NSFC) under contract No. 40925018; the National Basic Research Program (973 Program) under contract No. 2011CB403603.

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Wang, X., Huang, B. & Zhang, H. Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii . Acta Oceanol. Sin. 33, 85–91 (2014). https://doi.org/10.1007/s13131-014-0413-x

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