The marbled goby, Pomatoschistus marmoratus, as a promising species for experimental evolution studies

Abstract

Breeding and rearing the offspring through successive generations are mandatory in order to study evolutionary responses to anthropogenic impact in marine organisms. However, fish offer a limited number of marine model species that allow performing multigenerational experimental approaches. Here, we propose a novel breeding and rearing experimental model based on the marbled goby Pomatoschistus marmoratus (Risso 1810) which is representative of small (up to 65 mm total length), benthic species with a short life cycle. We devised a ‘full-sib/half-sib’ breeding design, and the resulting offspring were reared in captivity using a complex feeding protocol and a creative design of the tanks. Three families survived up to 160 days post-hatching (dph); one was reared at 24 °C and two at 18 °C. The families reared at 18 °C reached sexual maturity and spawned. The size range at sexual maturity of individuals reared in captivity was consistent with the one observed in nature. The possibility to complete the entire life cycle, from hatching to sexual maturity and spawning in P. marmoratus offers great perspectives for experimental evolution and quantitative genetics studies aimed at understanding the role of evolutionary processes in response to global changes.

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References

  1. Amaral, I. P., & Johnston, I. A. (2012). Experimental selection for body size at age modifies early life-history traits and muscle gene expression in adult zebrafish. Journal of Experimental Biology, 215(22), 3895–3904.

    CAS  Article  PubMed  Google Scholar 

  2. Buechel, S. D., Booksmythe, I., Kotrschal, A., Jennions, M. D., & Kolm, N. (2016). Artificial selection on male genitalia length alters female brain size. Proceedings of the Royal Society of London B, 283, 20161796.

    Article  Google Scholar 

  3. Burgess, S.C. & Marshall, D.J. (2011). Temperature-induced maternal effects and environmental predictability. Journal of Experimental Biology, 214, 2329–2336.

  4. Cato, J., & Brown, C. (2003). Marine ornamental species: collection, culture, and conservation. Ames: Iowa State Press.

    Book  Google Scholar 

  5. Charmantier, A., Garant, D., & Kruuk, L. E. B. (2014). Quantitative genetics in the wild. Oxford: Oxford University Press.

    Book  Google Scholar 

  6. Crozier, L.G. & Hutchings, J.A. (2014). Plastic and evolutionary responses to climate change in fish. Evolutionary Applications, 7, 68–87.

  7. Donelson, J. M., & Munday, P. L. (2015). Transgenerational plasticity mitigates the impact of global warming to offspring sex ratios. Global Change Biology, 21, 2954–2962.

    Article  PubMed  Google Scholar 

  8. Engström-Öst, J., & Candolin, U. (2007). Human-induced water turbidity alters selection on sexual displays in sticklebacks. Behavioral Ecology, 18, 393–398.

    Article  Google Scholar 

  9. Garland, T., & Rose, M. R. (2009). Experimental evolution—concepts, methods, and applications of selection experiments. Berkeley: University of California Press.

    Google Scholar 

  10. Guillaume, A. S., Monro, K., & Marshall, D. J. (2015). Transgenerational plasticity and environmental stress: do paternal effects act as a conduit or a buffer? Functional Ecology, 30, 1175–1184.

    Article  Google Scholar 

  11. Heino, M., Diaz Pauli, B., & Dieckmann, U. (2015). Fisheries-induced evolution. Annual Review of Ecology, Evolution, and Systematics, 46, 461–480.

    Article  Google Scholar 

  12. Helfman, G., Collette, B. B., Facey, D. E., & Bowen, B. W. (2009). The diversity of fishes: biology, evolution, and ecology. Boston: Wiley-Blackwell.

    Google Scholar 

  13. Holt, J., & Holt, S. A. (2000). Vertical distribution and role of physical processes in the feeding dynamics of two larval sciaenids S. Ocellatus and C. nebulosus. Marine Ecology Progress Series, 193, 181–190.

    Article  Google Scholar 

  14. Hutchings, J. A., & Fraser, D. J. (2008). The nature of fisheries- and farming-induced evolution. Molecular Ecology, 17, 294–313.

    Article  PubMed  Google Scholar 

  15. Kotrschal, A., Rogell, B., Bundsen, A., et al. (2013). Artificial selection on relative brain size in the guppy reveals costs and benefits of evolving a larger brain. Current Biology, 23(2), 168–171.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. Lynch, M. T., & Walsh, B. (1998). Genetics and analysis of quantitative traits. Massachusetts: Sinauer Associates, Inc..

    Google Scholar 

  17. Malvezzi, A. J., Murray, C. S., Feldheim, K. A., et al. (2015). A quantitative genetic approach to assess the evolutionary potential of a coastal marine fish to ocean acidification. Evolutionary Applications., 8(4), 352–362.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. Mazzoldi, C., & Rasotto, M. B. (2001). Extended breeding season in the marbled goby, Pomatoschistus marmoratus (Teleostei: Gobiidae), in the Venetian lagoon. Environmental Biology of Fishes, 61, 175–183.

    Article  Google Scholar 

  19. Mazzoldi, C., Poltronieri, C., & Rasotto, M. (2002). Egg size variability and mating system in the marbled goby Pomatoschistus marmoratus (Pisces: Gobiidae). Marine Ecology Progress Series, 233, 231–239.

    Article  Google Scholar 

  20. Munday, P. L., Warner, R. R., Monro, K., Pandolfi, J. M., & Marshall, D. J. (2013). Predicting evolutionary responses to climate change in the sea. Ecology Letters, 16, 1488–1500.

    Article  PubMed  Google Scholar 

  21. Nelson, J. S. (2006). Fishes of the world (4th ed.). Hoboken: John Wiley & Sons.

    Google Scholar 

  22. Olivotto, I., Yasumasu, S., Gioacching, G., Maradonna, F., Cionna, C., & Carnevali, O. (2004). Cloning and expression of high choriolytic enzyme, a component of the hatching enzyme system, during embryonic development of the marine ornamental teleost, Chrysiptera parasema. Marine Biology, 145, 1235–1241.

    CAS  Article  Google Scholar 

  23. Olivotto, I., Zenobi, A., Rollo, A., Migliarini, B., Avella, M., & Carnevali, O. (2005). Breeding, rearing and feeding studies in the cleaner goby Gobiosoma evelynae. Aquaculture, 250, 175–182.

    Article  Google Scholar 

  24. Panfili, J., de Pontual, H., Troadec, H., & Wright, P. J. (2002). Manual of fish sclerochronology. Brest: Ifremer-lRD coedition.

    Google Scholar 

  25. Patzner, R. A., Gonçalves, E. J., Hastings, P. A., & Kapoor, B. G. (2009). The biology of blennies. Enfield: Science Publishers.

    Book  Google Scholar 

  26. Patzner, R. A., Van Tassel, J. L., Kovačić, M., & Kapoor, B. G. (2011). The biology of gobies. Enfield: Science Publishers.

    Google Scholar 

  27. Pauls, S. U., Nowak, C., Bálint, M., & Pfenninger, M. (2013). The impact of global climate change on genetic diversity within populations and species. Molecular Ecology, 22, 925–946.

    Article  PubMed  Google Scholar 

  28. Risso, A. (1810). Ichthyologie de Nice, ou histoire naturelle des poissons du Département des Alpes Maritimes. F. Schoell, Paris. i-xxxvi + 1–388, Pls. 1–11.

  29. Salinas, S., & Munch, S. B. (2012). Thermal legacies: transgenerational effects of temperature on growth in a vertebrate. Ecology Letters, 15, 159–163.

    Article  PubMed  Google Scholar 

  30. van der Sluijs, I., Gray, S. M., Amorim, M. C. P., Barber, I., Candolin, U., Hendry, A. P., Krahe, R., et al. (2011). Communication in troubled waters: responses of fish communication systems to changing environments. Evolutionary Ecology, 25, 623–640.

    Article  Google Scholar 

  31. Wegner, K. M., Kalbe, M., & Reusch, T. B. H. (2007). Innate versus adaptive immunity in sticklebacks: evidence for trade-offs from a selection experiment. Evolutionary Ecololgy, 21, 473–4843.

    Article  Google Scholar 

  32. West-Eberhard, M. J. (2003). Developmental plasticity and evolution. New York: Oxford University Press.

    Google Scholar 

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Correspondence to Lisa Locatello.

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Funding

This study was supported by “Progetto Giovani Studiosi 2014” (2124PRGR14) from the University of Padova to LL.

Human and animal rights

The study did not involve endangered or protected species and was carried out in accordance with current Italian regulations for the use of animals in scientific procedures. Sampling and experimental procedures were reviewed and approved by the animal ethics committee of the University of Padova (OPBA, permission no. 134730). Animal collection in the field was authorised by Regione Veneto, Giunta Regionale (decreto 20, 14 March 2015).

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The authors declare that they have no competing interests.

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Locatello, L., Santon, M., Mazzoldi, C. et al. The marbled goby, Pomatoschistus marmoratus, as a promising species for experimental evolution studies. Org Divers Evol 17, 709–716 (2017). https://doi.org/10.1007/s13127-017-0339-1

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Keywords

  • Fish
  • Experimental evolution
  • Marbled goby
  • Rearing in captivity