Breeding and rearing the offspring through successive generations are mandatory in order to study evolutionary responses to anthropogenic impact in marine organisms. However, fish offer a limited number of marine model species that allow performing multigenerational experimental approaches. Here, we propose a novel breeding and rearing experimental model based on the marbled goby Pomatoschistus marmoratus (Risso 1810) which is representative of small (up to 65 mm total length), benthic species with a short life cycle. We devised a ‘full-sib/half-sib’ breeding design, and the resulting offspring were reared in captivity using a complex feeding protocol and a creative design of the tanks. Three families survived up to 160 days post-hatching (dph); one was reared at 24 °C and two at 18 °C. The families reared at 18 °C reached sexual maturity and spawned. The size range at sexual maturity of individuals reared in captivity was consistent with the one observed in nature. The possibility to complete the entire life cycle, from hatching to sexual maturity and spawning in P. marmoratus offers great perspectives for experimental evolution and quantitative genetics studies aimed at understanding the role of evolutionary processes in response to global changes.
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Amaral, I. P., & Johnston, I. A. (2012). Experimental selection for body size at age modifies early life-history traits and muscle gene expression in adult zebrafish. Journal of Experimental Biology, 215(22), 3895–3904.
Buechel, S. D., Booksmythe, I., Kotrschal, A., Jennions, M. D., & Kolm, N. (2016). Artificial selection on male genitalia length alters female brain size. Proceedings of the Royal Society of London B, 283, 20161796.
Burgess, S.C. & Marshall, D.J. (2011). Temperature-induced maternal effects and environmental predictability. Journal of Experimental Biology, 214, 2329–2336.
Cato, J., & Brown, C. (2003). Marine ornamental species: collection, culture, and conservation. Ames: Iowa State Press.
Charmantier, A., Garant, D., & Kruuk, L. E. B. (2014). Quantitative genetics in the wild. Oxford: Oxford University Press.
Crozier, L.G. & Hutchings, J.A. (2014). Plastic and evolutionary responses to climate change in fish. Evolutionary Applications, 7, 68–87.
Donelson, J. M., & Munday, P. L. (2015). Transgenerational plasticity mitigates the impact of global warming to offspring sex ratios. Global Change Biology, 21, 2954–2962.
Engström-Öst, J., & Candolin, U. (2007). Human-induced water turbidity alters selection on sexual displays in sticklebacks. Behavioral Ecology, 18, 393–398.
Garland, T., & Rose, M. R. (2009). Experimental evolution—concepts, methods, and applications of selection experiments. Berkeley: University of California Press.
Guillaume, A. S., Monro, K., & Marshall, D. J. (2015). Transgenerational plasticity and environmental stress: do paternal effects act as a conduit or a buffer? Functional Ecology, 30, 1175–1184.
Heino, M., Diaz Pauli, B., & Dieckmann, U. (2015). Fisheries-induced evolution. Annual Review of Ecology, Evolution, and Systematics, 46, 461–480.
Helfman, G., Collette, B. B., Facey, D. E., & Bowen, B. W. (2009). The diversity of fishes: biology, evolution, and ecology. Boston: Wiley-Blackwell.
Holt, J., & Holt, S. A. (2000). Vertical distribution and role of physical processes in the feeding dynamics of two larval sciaenids S. Ocellatus and C. nebulosus. Marine Ecology Progress Series, 193, 181–190.
Hutchings, J. A., & Fraser, D. J. (2008). The nature of fisheries- and farming-induced evolution. Molecular Ecology, 17, 294–313.
Kotrschal, A., Rogell, B., Bundsen, A., et al. (2013). Artificial selection on relative brain size in the guppy reveals costs and benefits of evolving a larger brain. Current Biology, 23(2), 168–171.
Lynch, M. T., & Walsh, B. (1998). Genetics and analysis of quantitative traits. Massachusetts: Sinauer Associates, Inc..
Malvezzi, A. J., Murray, C. S., Feldheim, K. A., et al. (2015). A quantitative genetic approach to assess the evolutionary potential of a coastal marine fish to ocean acidification. Evolutionary Applications., 8(4), 352–362.
Mazzoldi, C., & Rasotto, M. B. (2001). Extended breeding season in the marbled goby, Pomatoschistus marmoratus (Teleostei: Gobiidae), in the Venetian lagoon. Environmental Biology of Fishes, 61, 175–183.
Mazzoldi, C., Poltronieri, C., & Rasotto, M. (2002). Egg size variability and mating system in the marbled goby Pomatoschistus marmoratus (Pisces: Gobiidae). Marine Ecology Progress Series, 233, 231–239.
Munday, P. L., Warner, R. R., Monro, K., Pandolfi, J. M., & Marshall, D. J. (2013). Predicting evolutionary responses to climate change in the sea. Ecology Letters, 16, 1488–1500.
Nelson, J. S. (2006). Fishes of the world (4th ed.). Hoboken: John Wiley & Sons.
Olivotto, I., Yasumasu, S., Gioacching, G., Maradonna, F., Cionna, C., & Carnevali, O. (2004). Cloning and expression of high choriolytic enzyme, a component of the hatching enzyme system, during embryonic development of the marine ornamental teleost, Chrysiptera parasema. Marine Biology, 145, 1235–1241.
Olivotto, I., Zenobi, A., Rollo, A., Migliarini, B., Avella, M., & Carnevali, O. (2005). Breeding, rearing and feeding studies in the cleaner goby Gobiosoma evelynae. Aquaculture, 250, 175–182.
Panfili, J., de Pontual, H., Troadec, H., & Wright, P. J. (2002). Manual of fish sclerochronology. Brest: Ifremer-lRD coedition.
Patzner, R. A., Gonçalves, E. J., Hastings, P. A., & Kapoor, B. G. (2009). The biology of blennies. Enfield: Science Publishers.
Patzner, R. A., Van Tassel, J. L., Kovačić, M., & Kapoor, B. G. (2011). The biology of gobies. Enfield: Science Publishers.
Pauls, S. U., Nowak, C., Bálint, M., & Pfenninger, M. (2013). The impact of global climate change on genetic diversity within populations and species. Molecular Ecology, 22, 925–946.
Risso, A. (1810). Ichthyologie de Nice, ou histoire naturelle des poissons du Département des Alpes Maritimes. F. Schoell, Paris. i-xxxvi + 1–388, Pls. 1–11.
Salinas, S., & Munch, S. B. (2012). Thermal legacies: transgenerational effects of temperature on growth in a vertebrate. Ecology Letters, 15, 159–163.
van der Sluijs, I., Gray, S. M., Amorim, M. C. P., Barber, I., Candolin, U., Hendry, A. P., Krahe, R., et al. (2011). Communication in troubled waters: responses of fish communication systems to changing environments. Evolutionary Ecology, 25, 623–640.
Wegner, K. M., Kalbe, M., & Reusch, T. B. H. (2007). Innate versus adaptive immunity in sticklebacks: evidence for trade-offs from a selection experiment. Evolutionary Ecololgy, 21, 473–4843.
West-Eberhard, M. J. (2003). Developmental plasticity and evolution. New York: Oxford University Press.
This study was supported by “Progetto Giovani Studiosi 2014” (2124PRGR14) from the University of Padova to LL.
Human and animal rights
The study did not involve endangered or protected species and was carried out in accordance with current Italian regulations for the use of animals in scientific procedures. Sampling and experimental procedures were reviewed and approved by the animal ethics committee of the University of Padova (OPBA, permission no. 134730). Animal collection in the field was authorised by Regione Veneto, Giunta Regionale (decreto 20, 14 March 2015).
Conflict of interest
The authors declare that they have no competing interests.
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Locatello, L., Santon, M., Mazzoldi, C. et al. The marbled goby, Pomatoschistus marmoratus, as a promising species for experimental evolution studies. Org Divers Evol 17, 709–716 (2017). https://doi.org/10.1007/s13127-017-0339-1
- Experimental evolution
- Marbled goby
- Rearing in captivity