Organisms Diversity & Evolution

, Volume 17, Issue 2, pp 375–391

A new giant egg-laying onychophoran (Peripatopsidae) reveals evolutionary and biogeographical aspects of Australian velvet worms

Original Article

DOI: 10.1007/s13127-016-0321-3

Cite this article as:
Oliveira, I.S. & Mayer, G. Org Divers Evol (2017) 17: 375. doi:10.1007/s13127-016-0321-3

Abstract

Representatives of Ooperipatellus (Peripatopsidae) are among the smallest onychophorans known, commonly varying between 10 and 20 mm in length. Herein, we present a peculiar new species of Ooperipatellus from Tasmania that can exceed twice the length of other representatives of this taxon. Ooperipatellus nickmayerisp. nov. is comprehensively described based on morphological, molecular, karyological and slime protein profile data. Morphological analyses expose a set of novel features in this species, including a swollen area covered with a modified integument on the posterior border of the male genital pad, modified papillae on the female ovipositor and the presence of pseudoplicae in the dorsal integument. The evolutionary significance of pseudoplicae remains unclear, but similarities between O. nickmayerisp. nov. and Plicatoperipatus jamaicensis, the only species from which these structures were previously known, suggest they evolved due to functional constraints of the onychophoran integument. Our karyological investigation further revealed that the new species has the largest karyotype known within Peripatopsidae (2n = 50, XY). Finally, the results of our molecular phylogenetic analyses support the recognition of O. nickmayerisp. nov. and shed light on previously unclear aspects of the biogeographical history of Ooperipatellus in Southern Australia, Tasmania and New Zealand.

Keywords

Biogeography Onychophora Ooperipatellus Peripatopsidae Tasmania 

Supplementary material

13127_2016_321_Fig11_ESM.gif (723 kb)
Figure S1

Arrangement of dermal papillae along the dorsal midline inOoperipatellus nickmayerisp. nov. Scanning electron micrographs. In all images, the anterior orientation is at the top of the figure, with posterior at the bottom. (A) Overview of dorsal integument. Arrows point to pseudoplicae composed solely of accessory papillae. (B) Detail of the same image as in A, artificially coloured. Pseudoplicae are represented in purple, primary papillae in blue, and accessory papillae in orange. The pattern formed by primary and accessory papillae repeats along the entire dorsal midline (dotted line), irrespective of whether or not pseudoplicae are present. Abbreviations: ac, accessory papilla; pp., primary papilla. (GIF 723 kb)

13127_2016_321_MOESM1_ESM.tif (9.1 mb)
High resolution image (TIFF 9331 kb)
13127_2016_321_Fig12_ESM.gif (54 kb)
Figure S2

Phylogenetic relationships among representative onychophorans, includingOoperipatellus nickmayerisp. nov. Maximum likelihood topology combining the 12S rRNA, 16S rRNA, 18S rRNA and 28S rRNA nucleotide sequences with translated amino acids of COI sequences. Four species of Peripatidae were used as outgroup taxa. Bootstrap values are provided above the branches. Asterisks indicate maximum bootstrap support values (=100). (GIF 53 kb)

13127_2016_321_MOESM2_ESM.tif (187 kb)
High resolution image (TIFF 186 kb)
13127_2016_321_Fig13_ESM.gif (53 kb)
Figure S3

Phylogenetic relationships among representative onychophorans, includingOoperipatellus nickmayerisp. nov. Maximum likelihood topology combining the 12S rRNA, 16S rRNA, 18S rRNA and 28S rRNA nucleotide sequences with COI nucleotide sequences. Four species of Peripatidae were used as outgroup taxa. Bootstrap values are provided above the branches. Asterisks indicate maximum bootstrap support values (=100). (GIF 53 kb)

13127_2016_321_MOESM3_ESM.tif (188 kb)
High resolution image (TIFF 187 kb)

Funding information

Funder NameGrant NumberFunding Note
Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • 290029/2010-4
Deutsche Forschungsgemeinschaft (DE)
  • Ma 4147/3-1

Copyright information

© Gesellschaft für Biologische Systematik 2017

Authors and Affiliations

  1. 1.Department of Zoology, Institute of BiologyUniversity of KasselKasselGermany
  2. 2.Departamento de Zoologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil

Personalised recommendations