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A vanishing hotspot—the impact of molecular insights on the diversity of Central European Bythiospeum Bourguignat, 1882 (Mollusca: Gastropoda: Truncatelloidea)

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Abstract

The current study investigates the Central European groundwater snails of the genus Bythiospeum which are currently regarded as highly diverse with a hotspot in south-western Germany coupled with high rates of endemism. This systematic concept combined with the fragility of their habitat results in a high rate of threatened species (76–96 %). The analyses of the mitochondrial COI fragment of nearly 200 specimens from 35 localities in Germany, Switzerland and marginally Austria and France including type localities of 14 taxa revealed five well-distinguished clades with genetic distances from 3.6 to 16.4 % while variability within clades and populations is very low. Morphological investigations focused on parts of the reproductive systems showed rather great uniformity with only minor differences in penis shape, size and localisation. Previous species concepts based on conchology and morphology do not correspond to the current results, and shell shape is suspected as poor indicator for species delineation. Our data indicate a significantly lower diversity in Central Europe, e.g. only three species occurring in Germany instead of 25. Taxonomic implications of our hypothesis are given and discussed. Accordingly, most of the German populations belong to Bythiospeum acicula (Held, 1838) while B. husmanni (C. R. Boettger, 1963) is a very restricted relict. In comparison with other groundwater organisms, two of the more northerly distributed species show a large range stretching over at least 410 or 490 km respectively. Post-glacial re-colonisation from refugia is discussed as most likely explanation of the recent distribution pattern.

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Acknowledgments

The following people and organisations helped collecting material, gave valuable hints to localities or allowed access to their properties: ARGE Blautopf, Dr. Klaus Dobat, Tübingen, Margrit and Gerhard Falkner, Hörlkofen, Klaus Groh, Bad Dürkheim, Ralf Hanneforth, Schwerte, Hajo Kobialka, Höxter, Hannes Köble, Köngen (Landesverband für Höhlen- und Karstforschung BW e. V.), Achim Lehmkuhl, SMNS, Uwe Maigler, Wendlingen, Dr. Wolfgang Rähle, Tübingen, Thomas Rathgeber, formerly SMNS, Carsten Renker, Harxheim, Anette Rosenbauer, Backnang, Jörg Rüetschi, Bern, Ulf Stein, Landratsamt Esslingen, Rainer Straub, Filderstadt, Pascal Stucki, Neuchâtel, Ewald Wehrle, Rheinfelden (Tschamberhöhle), Dieter Weber, Haßloch, Gerhard Weitmann, Mainz, Burkhard Westphal and the owners of the sources in Mühlheim, Oberminseln and Dossenbach. Special thanks go to Anette Rosenbauer who supported YM in his local collecting efforts. Prof. Dr. Thomas Wilke, Justus-Liebig-Universität Giessen and his lab members confirmed the poor condition of DNA in older specimens. Prof. Dr. Annette Reineke, Hochschule Geisenheim University, granted access to lab facilities for the molecular experiments conducted by JK. Emmanuel Tardy, Muséum d’histoire naturelle de la Ville de Genève, kindly supplied information regarding the material of Bernasconi and provided some anatomical material for comparison by loan. Dr. Rähle generously shared his experience and insight in various discussions, especially on molluscs throughout the Ice Ages. This study was partially funded by a Research Award from the Malacological Society of London to YM. GBOL Munich (Prof. Dr. Haszprunar, Jerome Moriniere, Katrin Koller) financially supported part of the sequencing in exchange for the sequence data.

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Richling, I., Malkowsky, Y., Kuhn, J. et al. A vanishing hotspot—the impact of molecular insights on the diversity of Central European Bythiospeum Bourguignat, 1882 (Mollusca: Gastropoda: Truncatelloidea). Org Divers Evol 17, 67–85 (2017). https://doi.org/10.1007/s13127-016-0298-y

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