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Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice

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Abstract

Alcoholic liver disease (ALD) is a common and serious threat to human health worldwide. In this study, the hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice was examined. Mice with alcohol-induced hepatotoxicity were treated intragastrically with gastrodin (50, 80, or 100 mg/kg). The mice treated with gastrodin experienced better outcomes than those who received only one dose of alcohol (50%, 10 mL/kg b.w.). Gastrodin treatment reduced the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), decreased hepatic malondialdehyde (MDA) content, and increased hepatic superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities in a dose-dependent manner. Gastrodin also alleviated histopathological changes induced by alcohol. Gastrodin protected against alcohol-induced increases in expression levels of the cytochrome P450 2E1 (CYP2E1) and mRNA levels of chemokine (C-X-C motif) ligand 1 (CXCL-1), interferon-γ (IFN-γ), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), vascular cell adhesion molecule 1 (VCAM-1), nuclear factor-kappa B (NF-κB), Toll-like receptor 4 (TLR-4), and activator of transcription 3 (STAT-3). Moreover, gastrodin-increased nuclear transcription factor 2 (Nrf2) translocates to the nucleus and enhanced the activity of anti-oxidant enzymes, and could thereby ameliorate alcohol-induced liver injury in mice. This study demonstrated that gastrodin may be an effective therapeutic agent against alcohol-induced liver injury.

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Funding

This work was supported by Construction Project of Shaanxi Collaborative Innovation Center/Qinling-Bashan Mountains Bioresources Comprehensive Development and Collaborative Innovation (QBXT-Z(Z)-15-1, QBXT-Z(Z)-15-21), the High-end Foreign Experts Recruitment Program of State Administration of Foreign Experts Affairs (GDT20176100048, GDT20186100426), Shaanxi Science and Technology Coordinating Innovation Engineering Program (2015KTTSSF01-03, 2015HBGC-18), Shaanxi Provincial Science and Technology Research Development Program (2017NY180), Shaanxi Province Key Research and Development Plan (2017SF-074), The Scientific Research Program of the Shaanxi Provincial Education Department (17JK0135), and Shaanxi University of Technology Master Graduate Innovation Project (SLGYCX1833).

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Authors and Affiliations

  1. Chinese-German Joint Laboratory for Natural Product Research, College of Biological Science and Engineering, Qingling-Bashan Mountains Bioresources Comprehensive Development C.I.C, Shaanxi University of Technology, East on the 1st Ring Road, Hanzhong, 723000, Shaanxi Province, China

    Xin-Xin Li, Zhi-Hui Jiang, Bo Zhou, Chen Chen & Xiao-Ying Zhang

  2. College of Veterinary Medicine, Northwest A&F University (North Campus), Xinong Rd. 22, Post Box 19, Yangling, 712100, Shaanxi Province, China

    Xiao-Ying Zhang

  3. Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Xiao-Ying Zhang

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  1. Xin-Xin Li
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Correspondence to Chen Chen or Xiao-Ying Zhang.

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All animal experimental protocols were reviewed and approved by the Ethics Committee of Shaanxi University of Technology for the use of laboratory animals.

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Li, XX., Jiang, ZH., Zhou, B. et al. Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice. J Physiol Biochem 75, 29–37 (2019). https://doi.org/10.1007/s13105-018-0647-8

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  • DOI: https://doi.org/10.1007/s13105-018-0647-8

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