Effects of pterostilbene in brown adipose tissue from obese rats

  • Leixuri Aguirre
  • Iñaki Milton-Laskibar
  • Elizabeth Hijona
  • Luis Bujanda
  • Agnes M. Rimando
  • María P. Portillo
Original Article

DOI: 10.1007/s13105-017-0556-2

Cite this article as:
Aguirre, L., Milton-Laskibar, I., Hijona, E. et al. J Physiol Biochem (2017). doi:10.1007/s13105-017-0556-2

Abstract

In recent years, much attention has been paid by the scientific community to phenolic compounds as active biomolecules naturally present in foods. Pterostilbene is a resveratrol dimethylether derivative which shows higher bioavailability. The aim of the present study was to analyze the effect of pterostilbene on brown adipose tissue thermogenic markers in a model of genetic obesity, which shows reduced thermogenesis. The experiment was conducted with 30 Zucker (fa/fa) rats that were distributed in three experimental groups: control and two groups orally administered with pterostilbene at 15 and 30 mg/kg body weight/day for 6 weeks. Gene expression of uncoupling protein 1 (Ucp1), peroxisome proliferator-activated receptor γ co-activator 1 α (Pgc-1α), carnitine palmitoyl transferase 1b (Cpt1b), peroxisome proliferator-activated receptor α (Pparα), nuclear respiratory factor 1 (Nfr1), and cyclooxygenase-2 (Cox-2); protein expression of PPARα, PGC-1α, p38 mitogen-activated protein kinase (p38 MAPK), UCP1 and glucose transporter (GLUT4); and enzyme activity of CPT 1b and citrate synthase (CS) were assessed in interscapular brown adipose tissue. With the exception of Pgc-1α expression, all these parameters were significantly increased by pterostilbene administration. These results show for the first time that pterostilbene increases thermogenic and oxidative capacity of brown adipose tissue in obese rats. Whether these effects effectively contribute to the antiobesity properties of these compound needs further research.

Keywords

Pterostilbene Brown adipose tissue Obese rats Fatty acid oxidation GLUT4 

Funding information

Funder NameGrant NumberFunding Note
Ministerio de Economía y Competitividad
  • (AGL-2015-65719-R)
Fondo Europeo de Desarrollo Regional
  • (FEDER)
Instituto de Salud Carlos III
    Government of the Basque Country
    • (IT-572-13)
    University of the Basque Country (UPV/EHU)
    • (ELDUNANOTEK UFI11/32)

    Copyright information

    © University of Navarra 2017

    Authors and Affiliations

    • Leixuri Aguirre
      • 1
      • 2
    • Iñaki Milton-Laskibar
      • 1
    • Elizabeth Hijona
      • 3
      • 4
    • Luis Bujanda
      • 3
      • 4
    • Agnes M. Rimando
      • 5
    • María P. Portillo
      • 1
      • 2
    1. 1.Nutrition and Obesity Group, Department of Nutrition and Food Sciences, Faculty of PharmacyUniversity of the Basque Country (UPV/EHU) and Lucio Lascaray Research CentreVitoriaSpain
    2. 2.CIBER Obesity and Physiopathology of Nutrition (CIBERobn)Institute of Health Carlos IIIMadridSpain
    3. 3.Department of GastroenterologyUniversity of the Basque Country (UPV/EHU), Donostia Hospital and Biodonostia InstituteSan SebastiánSpain
    4. 4.CIBER Hepatic and Digestive Pathologies (CIBERehd)Institute of Health Carlos IIIMadridSpain
    5. 5.United States Department of Agriculture, Agricultural Research ServiceNatural Products Utilization Research UnitUniversityUSA

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