Journal of Physiology and Biochemistry

, Volume 72, Issue 2, pp 157–167 | Cite as

Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice

  • Youngshim Choi
  • Suhyeon Jang
  • Myung-Sook Choi
  • Zae Young Ryoo
  • Taesun ParkEmail author
Original Paper


Fibroblast growth factors (FGFs) are pleiotropic growth factors that control cell proliferation, migration, and differentiation. Herein, we evaluated whether visceral adiposity of mice is accompanied by the alteration of signaling molecules mediated by fibroblast growth factor receptor 1 (FGFR1) induced by using two different male C57BL/6J mice models of obesity namely high-fat diet (HFD)-induced obesity for 12 weeks or mice with genetic deletion of leptin (ob/ob). Both HFD-fed and ob/ob mice exhibited significantly higher messenger RNA (mRNA) levels of FGF1, cyclin D (cycD), transcription factor E2F1, peroxisome proliferator-activated receptor-gamma 2 (PPAR-γ2), CCAAT-enhancer-binding protein alpha (C/EBPα), and adipocyte protein 2 (aP2) genes in their epididymal adipose tissues compared to those of the normal diet (ND)-fed and lean control mice, respectively. In addition, immunoblot analyses of the epididymal adipose tissues revealed that both mice exposed to HFD and ob/ob mice exhibited elevated phosphorylation of FGFR1, extracellular-signal-regulated kinase (ERK), and retinoblastoma (Rb) proteins. These data support the notion that FGF1-mediated signaling represents an important signaling cascade related to adipogenesis, at least partially, among other known signaling pathways. These new findings regarding the molecular mechanisms controlling adipose tissue plasticity provide a novel insight about the functional network with potential therapeutic application against obesity.


FGF1 Adipogenesis High-fat diet Obesity Adipose tissue 



Adipocyte protein 2


Bone morphogenetic proteins


CCAAT-enhancer-binding protein alpha


Cyclin D


Extracellular-signal-regulated kinase


Fatty acid synthase


Fibroblast growth factors


Fibroblast growth factor receptor 1


Glycogen synthase kinase 3


High-fat diet


Lipoprotein lipase


Microvascular endothelial cells


Normal diet


Peroxisome proliferator-activated receptor-gamma 2






Mothers against decapentaplegic homolog 3


Sterol regulatory element-binding transcription factor 1c


Transforming growth factor-beta



This research was supported by Technology Commercialization Support Program (Program no. 1130373), Ministry of Agriculture, Food and Rural Affairs, and SRC program (Center for Food & Nutritional Genomics: Grant no. 2015R1A5A6001906) of the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology.

Compliance with ethical standards

Ethics statement

All animal experiments were performed humanely according to Korean Food and Drug Administration (KFDA) guidelines. All mouse experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the Yonsei Laboratory Animal Research Center (YLARC) (Permit no.: 2011-0061). All mice were maintained in the specific pathogen-free facility of the YLARC.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

13105_2016_468_MOESM1_ESM.docx (13 kb)
Supplementary Table 1 Composition of the experimental diet (g/kg) fed to mice. (DOCX 13 kb)
13105_2016_468_MOESM2_ESM.doc (38 kb)
Supplementary Table 2 Primer sequences used for semi-quantitative RT-PCR analysis. (DOC 37 kb)


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Copyright information

© University of Navarra 2016

Authors and Affiliations

  • Youngshim Choi
    • 1
  • Suhyeon Jang
    • 1
  • Myung-Sook Choi
    • 2
  • Zae Young Ryoo
    • 3
  • Taesun Park
    • 1
    Email author
  1. 1.Department of Food and NutritionYonsei UniversitySeodaemun-guRepublic of Korea
  2. 2.Department of Food Science and NutritionKyungpook National UniversityDaeguRepublic of Korea
  3. 3.School of Life Science and BiotechnologyKyungpook National UniversityDaeguRepublic of Korea

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