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Oxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanism

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Abstract

In addition to its well-known effects on parturition and lactation, oxytocin (OT) plays an important role in modulation of pain and nociceptive transmission. But, the mechanism of this effect is unclear. To address the possible role of OT on pain modulation at the peripheral level, the effects of OT on intracellular calcium levels ([Ca2+]i) in rat dorsal root ganglion (DRG) neurons were investigated by using an in vitro calcium imaging system. DRG neurons were grown in primary culture following enzymatic and mechanical dissociation of ganglia from 1- or 2-day-old neonatal Wistar rats. Using the fura-2-based calcium imaging technique, the effects of OT on [Ca2+]i and role of the protein kinase C (PKC)-mediated pathway in OT effect were assessed. OT caused a significant increase in basal levels of [Ca2+]i after application at the doses of 30 nM (n = 34, p < 0.01), 100 nM (n = 41, p < 0.001) and 300 nM (n = 46, p < 0.001). The stimulatory effect of OT (300 nM) on [Ca2+]i was persistent in Ca2+-free conditions (n = 56, p < 0.01). Chelerythrine chloride, a PKC inhibitor, significantly reduced the OT-induced increase in [Ca2+]i (n = 28, p < 0.001). We demonstrated that OT activates intracellular calcium signaling in cultured rat primary sensory neurons in a dose- and PKC-dependent mechanism. The finding of the role of OT in peripheral pain modification may serve as a novel target for the development of new pharmacological strategies for the management of pain.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

    Ahmet Ayar

  2. Department of Biophysics, Faculty of Medicine, Firat University, TR23119, Elazig, Turkey

    Mete Ozcan & Ihsan Serhatlioglu

  3. Department of Physiology Faculty of Medicine, Inonu University, Malatya, Turkey

    Ergul Alcin

  4. Department of Anaesthesiology, Elazig Education and Research Hospital, Elazig, Turkey

    Sibel Ozcan

  5. Department of Physiology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey

    Selim Kutlu

  6. Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey

    Haluk Kelestimur

Authors
  1. Ahmet Ayar
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  2. Mete Ozcan
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  3. Ergul Alcin
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  4. Ihsan Serhatlioglu
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  5. Sibel Ozcan
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  6. Selim Kutlu
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  7. Haluk Kelestimur
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Corresponding author

Correspondence to Mete Ozcan.

Additional information

A preliminary form of this study was presented in Physiology 2009 (6–10 July 2009, Main Meeting of The Physiological Society), Dublin, Ireland.

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Ayar, A., Ozcan, M., Alcin, E. et al. Oxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanism. J Physiol Biochem 70, 43–48 (2014). https://doi.org/10.1007/s13105-013-0278-z

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  • DOI: https://doi.org/10.1007/s13105-013-0278-z

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