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Positive influence of a natural product as propolis on antioxidant status and lipid peroxidation in senescent rats

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Abstract

Given the importance of oxidative stress associated to aging, it would be interesting to assess the effect of oral supplementation with antioxidant substances capable of diminishing oxidative aggression and free radicals generation associated to this condition. This study investigated the effects of AIN-93 M diet supplemented either with 2 % of propolis, or with 4 % of a natural product obtained from lyophilizate vegetables, selected by its antioxidant properties, in senescent healthy Wistar rats fed ad libitum over 3 months. Propolis supplementation leads to a lower level of glucose and cholesterol concentrations together with a reduction in protein oxidation. Plasma thiobarbituric acid-reactive substance levels were lower in the rats consuming the natural vegetable product and propolis possibly due to its antioxidant components, neutralizing the free radical produced, and thus preventing cellular damage. The results of the present study suggest a synergic effect of overall propolis compounds reducing the oxidative stress and glucose and cholesterol plasma levels associated with aging.

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References

  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 50:121–127

    Article  Google Scholar 

  2. Aga M, Arai N, Ohashi E, Ariyasu T, Arai S, Iwaki K, Ohta T, Fukuda S (2009) Propolis enhances adipocyte differentiation and prevents insulin resistance in 3T3-L1 cells (propolis improves insulin resistance in vitro). J Jpn Soc Food Sci Tech 56:31–39

    Article  CAS  Google Scholar 

  3. Ballatori N, Krance SM, Notenboom S, Shi S, Tieu K, Hammond CL (2009) Glutathione dysregulation and the etiology and progression of human diseases. Biol Chem 390:191–214

    Article  PubMed  CAS  Google Scholar 

  4. Beyraghdar Kashkooli O, Ebrahimi Dorcheh E, Mahboobi-Soofiani N, Samie A (2011) Long-term effects of propolis on serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Ecotoxicol Environ Saf 74:315–318

    Article  PubMed  Google Scholar 

  5. Bohr V (2002) Oxidative DNA damage and repair. Free Radic Biol Med 32:804–812

    Article  PubMed  CAS  Google Scholar 

  6. Buettner GR (1993) The pecking order of free radicals and antioxidants: lipid peroxidation, alpha-tocopherol, and ascorbate. Arch Biochem Biophys 300:535–543

    Article  PubMed  CAS  Google Scholar 

  7. Burdock GA (1998) Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 36:347–363

    Article  PubMed  CAS  Google Scholar 

  8. Castro SL (2001) Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. Ann Rev Biomed Sci 3:49–83

    Google Scholar 

  9. Chen Q, Fischer A, Reagan J, Yan L, Ames B (1995) Oxidative DNA damage and senescence of human diploid fibroblast cells. Proc Natl Acad Sci USA 92:4337–4341

    Article  PubMed  CAS  Google Scholar 

  10. Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress, and the biology of ageing. Nature 408:239–247

    Article  PubMed  CAS  Google Scholar 

  11. Glei M, Pool-Zobel BL (2006) The main catechin of green tea, (−)-epigallocatechin-3-gallate (EGCG), reduces bleomycin-induced DNA damage in human leucocytes. Toxicol In Vitro 20:295–300

    Article  PubMed  CAS  Google Scholar 

  12. Gómez-Romero M, Arráez-Román D, Moreno-Torres R, García-Salas P, Segura-Carretero A, Fernández-Gutiérrez A (2007) Antioxidant compounds of propolis determined by capillary electrophoresis-mass spectrometry. J Sep Sci 30:595–603

    Article  PubMed  Google Scholar 

  13. Grassmann J (2005) Terpenoids as plant antioxidants. Vitam Horm 72:505–535

    Article  PubMed  CAS  Google Scholar 

  14. Han LK, Sumiyoshi M, Zhang J, Liu MX, Zhang XF, Zheng YN (2003) Anti-obesity action of Salix matsudana leaves (Part 1). Anti-obesity action by polyphenols of Salix matsudana in high fat-diet treated rodent animals. Phytother Res 17:1188–1194

    Article  PubMed  Google Scholar 

  15. Hanahan DJ, Ekholm JE (1974) The preparation of red cell ghosts (membranes). Methods Enzymol 31:168–172

    Article  PubMed  CAS  Google Scholar 

  16. Hanasaki Y, Ogawa S, Fukui S (1994) The correlation between active oxygens scavenging and antioxidative effects of flavonoids. Free Radic Biol Med 16:845–850

    Article  PubMed  CAS  Google Scholar 

  17. Ichi I, Hori H, Takashima Y, Adachi N, Kataoka R, Okihara K, Hashimoto K, Kojo S (2009) The beneficial effect of propolis on fat accumulation and lipid metabolism in rats fed a high-fat diet. J Food Sci 74:H127–H131

    Article  PubMed  CAS  Google Scholar 

  18. Igarashi K, Ohmuma M (1995) Effects of isorhamnetin, rhamnetin, and quercetin on the concentrations of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities of rats. Biosci Biotechnol Biochem 59:595–601

    Article  PubMed  CAS  Google Scholar 

  19. Kamata H, Hirata H (1999) Redox regulation of cellular signalling. Cell Signal 11:1–14

    Article  PubMed  CAS  Google Scholar 

  20. Kregel KC, Zhang HJ (2007) An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations. Am J Physiol Regul Integr Comp Physiol 292:18–36

    Article  Google Scholar 

  21. Lowry OH, Rosenburgh NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–270

    PubMed  CAS  Google Scholar 

  22. Mari M, Morales A, Colell A, García-Ruiz C, Fernández-Checa JC (2009) Mitochondrial glutathione, a key survival antioxidant. Antioxid Redox Signal 11:2685–2700

    Article  PubMed  CAS  Google Scholar 

  23. Middleton E Jr, Kandaswami C, Theoharides TC (2000) The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 52:673–751

    PubMed  CAS  Google Scholar 

  24. Mishima S, Yoshida C, Akino S, Sakamoto T (2005) Antihypertensive effects of Brazilian propolis: identification of caffeoylquinic acids as constituents involved in the hypotension in spontaneously hypertensive rats. Biol Pharm Bull 28:1909–1914

    Article  PubMed  CAS  Google Scholar 

  25. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  26. Pietta PG (2010) Flavonoids as antioxidants. J Nat Prod 63:1035–1042

    Article  Google Scholar 

  27. Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 Purified diets for laboratory rodents: final report of the American Institute of Nutrition and Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951

    PubMed  CAS  Google Scholar 

  28. Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363

    Article  PubMed  CAS  Google Scholar 

  29. Salatino A, Fernandes-Silva CC, Righi AA, Salatino MLF (2011) Propolis research and the chemistry of plant products. Nat Prod Rep 28:925–936

    Article  PubMed  CAS  Google Scholar 

  30. Sforcin JM, Novelli ELB, Funari SRC (2002) Seasonal effect of Brazilian propolis on seric biochemical variables. J Venom Anim Toxins 8:244–254

    Article  Google Scholar 

  31. Singelton VR, Orthifer R, Lamuela-Raventos R (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  Google Scholar 

  32. Ueda M, Hayashibara K, Ashida H (2013) Propolis extract promotes translocation of glucose transporter 4 and glucose uptake through both PI3K- and AMPK-dependent pathways in skeletal muscle. Biofactor. doi:10.1002/biof.1085

    Google Scholar 

  33. Vilenchik M, Knudson A (2003) Endogenous DNA double-strand breaks: production, fidelity of repair, and induction of cancer. Proc Natl Acad Sci USA 100:12871–12876

    Article  PubMed  CAS  Google Scholar 

  34. Worthington DJ, Rosemeyer MH (1974) Human glutathione reductase: purification of the crystalline enzyme from erythrocytes. Eur J Biochem 48:167–177

    Article  PubMed  CAS  Google Scholar 

  35. Yagi K (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 15:212–216

    Article  PubMed  CAS  Google Scholar 

  36. Zamami Y, Takatori S, Koyama T, Goda M, Iwatani Y, Doi S, Kawasaki H (2007) Effect of propolis on insulin resistance in fructose-drinking rats. Yakugaku Zasshi 127:2065–2073

    Article  PubMed  CAS  Google Scholar 

  37. Zhishen J, Mengcheng T, Jianming W (1999) Research on antioxidant activity of flavonoids from natural materials. Food Chem 64:555–559

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiotherapy, Faculty of Health Sciences, University of Granada, 18071, Granada, Spain

    Cristina Lisbona

  2. Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology “José Mataix”, 18071, Granada, Spain

    Javier Díaz-Castro, María J. M. Alférez & Inmaculada López-Aliaga

  3. Department of Nursing, Faculty of Health Sciences, University of Granada, 18071, Granada, Spain

    Isabel M. Guisado & Rafael Guisado

  4. Department of Physiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071, Granada, Spain

    Inmaculada López-Aliaga

Authors
  1. Cristina Lisbona
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  2. Javier Díaz-Castro
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  3. María J. M. Alférez
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  4. Isabel M. Guisado
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  5. Rafael Guisado
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  6. Inmaculada López-Aliaga
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Correspondence to Inmaculada López-Aliaga.

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Lisbona, C., Díaz-Castro, J., Alférez, M.J.M. et al. Positive influence of a natural product as propolis on antioxidant status and lipid peroxidation in senescent rats. J Physiol Biochem 69, 919–925 (2013). https://doi.org/10.1007/s13105-013-0270-7

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  • DOI: https://doi.org/10.1007/s13105-013-0270-7

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