Journal of Physiology and Biochemistry

, Volume 69, Issue 3, pp 539–546 | Cite as

Thiamine deficiency induces oxidative stress in brain mitochondria of Mus musculus

  • Anupama SharmaEmail author
  • Renu Bist
  • Parvesh Bubber
Original Paper


The present investigation evaluates the changes in the levels of antioxidant enzymes, lipid peroxidation (LPO), and protein carbonyl content (PCC) in brain mitochondria following thiamine deficiency (TD). The study was carried out on Mus musculus allocated into three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. The LPO was measured in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured biochemically. A significant increase in the TBARS (p < 0.0001) and PCC (p < 0.001) levels in group II (TD 8) and group III (TD 10) animals was observed in comparison to controls. The GSH levels were found to be reduced in both the treated groups compared to the control. A significant reduction in the activities of SOD was also observed in group II (p < 0.01) and group III (p < 0.0001) animals in comparison to the control. Enzymatic activities of CAT (p < 0.001) and GPx (p < 0.05) were found to be significantly reduced in group III (TD 10) in comparison to the control. In conclusion, reduction in the activities of antioxidant enzymes as well as an increase in LPO and PCC following TD implies oxidative stress in brain mitochondria that may further leads to neurodegeneration.


Antioxidant enzymes Brain mitochondria Lipid peroxidation Oxidative stress Protein carbonyl content Thiamine 



We acknowledge Prof. Aditya Shastri, Vice Chancellor, Banasthali University, Rajasthan for providing the suitable facilities to carry out the present investigation in the Department of Bioscience and Biotechnology.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© University of Navarra 2013

Authors and Affiliations

  1. 1.Department of Bioscience and BiotechnologyBanasthali UniversityJaipurIndia
  2. 2.Department of Bioscience and BiotechnologyBanasthali UniversityBanasthaliIndia
  3. 3.Department of Biochemistry, School of SciencesIndira Gandhi National Open UniversityNew DelhiIndia

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