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Dietary restriction improves systemic and muscular oxidative stress in type 2 diabetic Goto–Kakizaki rats

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Abstract

Type 2 diabetes is a heterogeneous metabolic disease characterized by insulin resistance and β-cell dysfunction leading to hyperglycaemia and dyslipidaemia. Dietary intervention seems to improve some of these cellular complications, namely insulin resistance. Our aim was to evaluate the effects of dietary restriction on systemic and skeletal muscle oxidative stress and insulin resistance in normal Wistar rats and Goto–Kakizaki (GK) rats, a non-obese type 2 diabetic animal model. Four-month-old normal and diabetic rats were separated in four groups. One group of each strain was maintained with ad libitum standard diet, and the other group was submitted to a dietary restriction (50% of control animals daily food intake), during 2 months. Metabolic profile, insulin resistance indexes and muscle lipids were determined. Oxidative stress parameters were also measured at systemic and muscle levels: protein carbonyl, 8-hydroxy-2′-deoxyguanosine and free 8-isoprostane. Dietary restriction improved lipid profile in both strains and urinary free 8-isoprostane and plasma carbonyl compounds in diabetic rats. An improvement of muscle triglycerides accumulation and 8-isoprostane concentration and a reduction of insulin resistance were also observed in GK rats. Our data show that dietary restriction ameliorates systemic and skeletal muscle oxidative stress state in type 2 diabetes, which is associated with improved insulin resistance.

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Acknowledgements

We thank Mário Simões from the Institute of Physiology, Faculty of Medicine of Coimbra for his technical support. We also thank Serviço de Patologia Clínica from the University Hospital of Coimbra for technical support. The present study was supported by the Faculty of Medicine, University of Coimbra and University Hospitals of Coimbra. We thank SERVIER Portugal for financial support.

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Institute of Physiology, Faculty of Medicine of University of Coimbra, Sub-unit 1, Polo III, Azinhaga de Santa Comba, Celas, 3000-354, Coimbra, Portugal

    Lisa Rodrigues, Joana Crisóstomo, Paulo Matafome, Teresa Louro, Elsa Nunes & Raquel Seiça

  2. Institute of Biomedical Research in Light and Image (IBILI), Faculty of Medicine, University of Coimbra, Coimbra, Portugal

    Lisa Rodrigues, Joana Crisóstomo, Paulo Matafome, Teresa Louro & Raquel Seiça

  3. Center of Ophthalmology, IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

    Paulo Matafome

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  1. Lisa Rodrigues
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  2. Joana Crisóstomo
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  3. Paulo Matafome
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  4. Teresa Louro
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  5. Elsa Nunes
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  6. Raquel Seiça
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Correspondence to Lisa Rodrigues.

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Rodrigues, L., Crisóstomo, J., Matafome, P. et al. Dietary restriction improves systemic and muscular oxidative stress in type 2 diabetic Goto–Kakizaki rats. J Physiol Biochem 67, 613–619 (2011). https://doi.org/10.1007/s13105-011-0108-0

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  • DOI: https://doi.org/10.1007/s13105-011-0108-0

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