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Age-related changes in xanthine oxidase activity and lipid peroxidation, as well as in the correlation between both parameters, in plasma and several organs from female mice

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Abstract

Xanthine oxidase, a purine catabolism enzyme, has been implicated as an important source of oxidant production and plays an essential role in several inflammatory and oxidative stress-related diseases. It is known that the increasing levels of oxidants cause the chronic oxidative stress characteristic of the ageing process. The aim of the present work was to determine the changes in xanthine oxidase activity and oxidative damage to lipids in several organs (liver, kidney, spleen, lung and two different brain areas, namely cerebral cortex and brainstem) and plasma from two different age groups of BALB/c female mice: adult (7-month-old) and old (18-month-old) mice, as well as to analyse the possible correlation between both parameters. Xanthine oxidase activity was significantly increased in liver, cerebral cortex and plasma from old mice in comparison with adults. Similar results were obtained in the lipid peroxidation levels, in which old mice showed a high increment in liver and cerebral cortex. Moreover, the results show a significant and positive correlation between xanthine oxidase activity and lipid peroxidation levels in cerebral cortex. The age-related increase in the xanthine oxidase activity and lipid peroxidation in liver and cerebral cortex of mice seems to suggest that the xanthine oxidase plays a role in the acceleration of the oxidative damage in these organs with age and its possible contribution to the pathophysiological changes associated to the process of ageing.

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References

  1. Al-Gonaiah M, Smith RA, Stone TW (2009) Xanthine oxidase-induced neuronal death via the oxidation of NADH: prevention by micromolar EDTA. Brain Res 1280:33–42

    Article  PubMed  CAS  Google Scholar 

  2. Alvarado C, Álvarez P, Jiménez L, De la Fuente M (2006) Oxidative stress in leukocytes from young prematurely aging mice is reversed by supplementation with biscuits rich in antioxidants. Dev Comp Immunol 30:1168–1180

    Article  PubMed  CAS  Google Scholar 

  3. Aranda R, Doménech E, Rus AD, Real JT, Sastre J, Viña J, Pallardó FV (2007) Age-related increase in xanthine oxidase activity in human plasma and rat tissues. Free Radic Res 41(11):1995–2000

    Article  Google Scholar 

  4. Basson AB, Terblanche SE, Oelofsen WA (1982) A comparative study on the effects of ageing and training on the levels of lipofuscin in the various tissues of the rat. Comp Biochem Physiol 71A:369–374

    Article  CAS  Google Scholar 

  5. Berry CE, Hare JM (2004) Xanthine oxidorreductase and cardiovascular disease: molecular mechanism and pathophysiological implications. J Physiol 555:589–606

    Article  PubMed  CAS  Google Scholar 

  6. Bokov A, Chaudhuri A, Richardson A (2004) The role of oxidative damage and stress in aging. Mech Ageing Dev 125:811–826

    Article  PubMed  CAS  Google Scholar 

  7. Boueiz A, Damarla M, Hassun PM (2008) Xanthine oxidoreductase in respiratory and cardiovascular disorders. Am J Physiol Lung Cell Mol Physiol 294:L830–L840

    Article  PubMed  CAS  Google Scholar 

  8. Cand F, Verdetti J (2003) Superoxide dismutase, glutathione peroxidase, catalase and lipid peroxidation in the major organs of the aging rats. Free Radic Biol Med 7(1):59–63

    Article  Google Scholar 

  9. Chung HY, Song SH, Hj K, Ikeno Y, Yu BP (1999) Modulation of renal xanthine oxidoreductase in aging: gene expression and reactive oxygen species generation. J Nutr Health Aging 3(1):19–23

    PubMed  CAS  Google Scholar 

  10. De la Fuente M, Miquel J (2009) An update of the oxidation-inflammation theory of aging. The involvement of the immune system in oxi-inflamm-aging. Curr Pharm Des 15(26):3003–3026

    Article  PubMed  Google Scholar 

  11. Desco M, Asensi M, Márquez R, Martínez-Vals J, Vento M, Pallardó FV, Sastre J, Viña J (2002) Xanthine oxidase is involved in free radical production in type 1 diabetes, protection by allopurinol. Diabetes 1(4):1118–1124

    Article  Google Scholar 

  12. Floyd RA, Hensley K (2002) Oxidative stress in brain aging. Implications for therapeutics of neurodegenerative diseases. Neurobiol Aging 23:795–807

    Article  PubMed  CAS  Google Scholar 

  13. Gredilla R, Barja G, López M (2001) Thyroid hormone-induced oxidative damage on lipids, glutathione and DNA in the mouse heart. Free Radic Res 35(4):417–425

    Article  PubMed  CAS  Google Scholar 

  14. Harman D (1956) Aging: a theory based on free radical and radiation chemistry. J Gerontol 11:298–300

    PubMed  CAS  Google Scholar 

  15. Harman D (1984) Free radical theory of aging. The "free radical" diseases. Age 7:111–131

    Article  CAS  Google Scholar 

  16. Harrison R (2002) Structure and function of xanthine oxidoreductase: where are we now? Free Radic Biol Med 6(33):774–796

    Article  Google Scholar 

  17. Harrison R (2004) Physiological roles of xanthine oxidorreductase. Drug Metab Rev 36(2):363–375

    Article  PubMed  CAS  Google Scholar 

  18. Knight JA (1995) Diseases related to oxygen-derived free radicals. Ann Clin Lab Sci 25:111–121

    PubMed  CAS  Google Scholar 

  19. Komaki Y, Sugiura H, Koarai A, Tomasi M, Ogawa H, Akita T, Hattori T, Ichinose M (2005) Cytokine-mediated xanthine oxidase upregulation in chronic obstructive pulmonary disease’s airways. Pulm Pharmacol Ther 18:297–302

    Article  PubMed  CAS  Google Scholar 

  20. Lewin MB, Timiras PS (1984) Lipid changes with aging in cardiac mitochondrial membranes. Mech Ageing Dev 24:343–351

    Article  PubMed  CAS  Google Scholar 

  21. Liu J, Mori A (1993) Age-associated changes in superoxide dismutase activity, thiobarbituric acid reactivity and reduced glutathione level in the brain and liver in senescence accelerated mice (SAM): a comparison with ddY mice. Mech Ageing Dev 71:23–30

    Article  PubMed  CAS  Google Scholar 

  22. Okuda S, Nishiyama N, Saito H, Katsuki H (1996) Hydrogen peroxide-mediated neuronal cell death induced by an endogenous neurotoxin, 3-hydroxykynurenine. Proc Natl Acad Sci U S A 93:12553–12558

    Article  PubMed  CAS  Google Scholar 

  23. Osarogiagbon UR, Choong S, Belcher JD, Vercellotti GM, Paller MS, Hebbel RP (2000) Reperfusion injury pathophysiology in sickle transgenic mice. Blood 96:314–320

    PubMed  CAS  Google Scholar 

  24. Parks DA, Granger DN (1986) Xanthine oxidase: biochemistry, distribution and physiology. Acta Physiol Scand Suppl 548:87–99

    PubMed  CAS  Google Scholar 

  25. Reiter RJ (1995) Oxidative processes and antioxidant defense mechanism in the aging brain. FASEB J 9:526–533

    PubMed  CAS  Google Scholar 

  26. Rikans LE, Hornbrook KR (1997) Lipid peroxidation, antioxidant protection and aging. Bio Biophys Acta 1362:116–127

    CAS  Google Scholar 

  27. Salminen A, Saari P, Kihlstrom M (1988) Age- and sex-related difference in lipid peroxidation of mouse cardiac and skeletal muscle. Comp Biochem Physiol 89B(4):695–689

    Google Scholar 

  28. Salmon AB, Richardson A, Pérez VI (2010) Update on the oxidative stress theory of aging: does oxidative stress play a role in aging or health aging? Free Radic Biol Med 48:642–655

    Article  PubMed  CAS  Google Scholar 

  29. Sastre J, Pallardó FV, Viña J (2003) The role of mitocondrial oxidative stress in aging. Free Radic Biol Med 35:1–8

    Article  PubMed  CAS  Google Scholar 

  30. Solaroglu I, Okutan O, Kaptonoglu E, Beskonakli E, Kilinc K (2005) Increased xanthine oxidase activity after traumatic brain injury in rats. J Clin Neurosci 12(3):273–275

    Article  PubMed  CAS  Google Scholar 

  31. Spiteller G (2001) Peroxidation of linoleic acid and its relation to aging and age dependent diseases. Mech Ageing Dev 122:617–657

    Article  PubMed  CAS  Google Scholar 

  32. Tian L, Cai Q, Wei H (1998) Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging. Free Radic Biol Med 24(9):1477–1484

    Article  PubMed  CAS  Google Scholar 

  33. Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278

    Article  PubMed  CAS  Google Scholar 

  34. Vanella A, Geremia E, D’urso G, Tiriolo P, Di Silvestro I, Grimaldi R, Pinturo R (1982) Superoxide dismutase activities in aging rat brain. J Gerontol 28(2):108–113

    Article  CAS  Google Scholar 

  35. Viña J, Gimeno A, Sastre J, Desco C, Asensi M, Pallardo FV, Cuesta A, Ferrero JA, Terada LS, Repine JE (2000) Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life 49(6):539–544

    Article  PubMed  Google Scholar 

  36. Wozniak A, Drewa B, Wozniak B, Schachtschabel DO (2004) Activity of antioxidant enzymes and concentration of lipid peroxidation products in selected tissues of mice of different ages, both healthy and melanoma-bearing. Z Fur Gerontol Geriatrie 37(3):184–189

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Ministry of Science and Innovation (BFU2008-04336), the Ministry of Health and Consumption (RETICEF, RD06/0013/003) of Spain and the Research Group of Madrid Complutense University (910379).

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Authors and Affiliations

  1. Department of Physiology (Animal Physiology II), Faculty of Biological Sciences, Madrid Complutense University, 28040, Madrid, Spain

    Carmen Vida, Isabel Corpas, Mónica De la Fuente & Eva M. González

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  1. Carmen Vida
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  2. Isabel Corpas
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  3. Mónica De la Fuente
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  4. Eva M. González
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Correspondence to Eva M. González.

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Vida, C., Corpas, I., De la Fuente, M. et al. Age-related changes in xanthine oxidase activity and lipid peroxidation, as well as in the correlation between both parameters, in plasma and several organs from female mice. J Physiol Biochem 67, 551–558 (2011). https://doi.org/10.1007/s13105-011-0100-8

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