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High glucose induced rat aorta vascular smooth muscle cell oxidative injury: involvement of protein tyrosine nitration

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Abstract

The alteration and further damage of vascular smooth muscle function have been implicated in the development of vascular complications and diabetes. Little is known about protein tyrosine nitration in vascular smooth muscle cell injury induced by high glucose. In this article, vascular smooth muscle cell was exposed to 30 and 40 mM high glucose for 72 h, and then the cell injury in vascular smooth muscle cell induced by high glucose was studied. It was found that high glucose stimulated vascular smooth muscle cell injury in a dose-dependent manner, including decreasing intracellular and extracellular glutathione contents, increasing malondialdehyde and intracellular reactive oxygen species content, increasing the production of nitric oxide (increased nitrite content in cell and medium), as well as increasing protein tyrosine nitration. By comparing protein tyrosine nitration induced by high glucose conditions and extrinsic factors (hemin–nitrite–glucose oxidase system and 3-morpholinosydnonimine), it may be speculated that protein is nitrated selectively, and specific protein tyrosine nitration is involved in diabetic vascular complications.

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Acknowledgment

This work was supported by grants from the National Natural Science Foundation of China (no. 30300073), the Research Found of the Department of Education of Zhejiang Province (Y201016792), the Program for Doctor Scientific Research Found of Zhejiang Normal University (ZC304009077), and New Century Excellent Talents in University (no. NCET-05-0649).

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Authors and Affiliations

  1. College of Chemical and Life Science, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China

    Yuling Zhao

  2. Department of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Yuling Zhao, Naihao Lu, Yan Zhang & Zhonghong Gao

  3. Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Yuling Zhao, Naihao Lu, Yan Zhang & Zhonghong Gao

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  1. Yuling Zhao
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Correspondence to Zhonghong Gao.

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Zhao, Y., Lu, N., Zhang, Y. et al. High glucose induced rat aorta vascular smooth muscle cell oxidative injury: involvement of protein tyrosine nitration. J Physiol Biochem 67, 539–549 (2011). https://doi.org/10.1007/s13105-011-0099-x

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  • DOI: https://doi.org/10.1007/s13105-011-0099-x

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