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Acetyl-l-carnitine prevents carbon tetrachloride-induced oxidative stress in various tissues of Wistar rats

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Abstract

Acetyl-l-carnitine (ALCAR) has been shown to prevent experimental selenite cataractogenesis, a manifestation of oxidative stress, but little is known about its potential in other settings of oxidative stress. The present study was based on the hypothesis that ALCAR prevents carbon tetrachloride (CCl4)-induced oxidative stress in vital tissues. Male albino Wistar rats were divided into three groups, each of six rats. Group I (control) rats received only vehicle (1 ml/kg b.w.) for 4 days; Group II (CCl4-exposed, untreated) rats received CCl4 (2 ml/kg b.w.) on the second and third days and vehicle on the first and fourth days; Group III (CCl4-exposed, ALCAR-treated) rats received ALCAR (200 mg/kg b.w.) for 4 days and CCl4 on the second and third days. All administrations were made intraperitoneally. After the experimental period, significantly (P < 0.05) elevated mean serum levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase were observed in Group II rats when compared to Group I and Group III rats. The mean levels of vitamin C, vitamin E, and reduced glutathione and the mean activities of superoxide dismutase, catalase, and glutathione peroxidase were significantly (P < 0.05) lower in samples of hemolysate and of liver, kidney, and brain tissues of Group II rats than those in Group I and Group III rats. The mean level of lipid peroxidation was significantly (P < 0.05) higher in Group II rats than that in Group I and Group III rats. Moreover, the CCl4-induced upregulation of inducible nitric oxide synthase expression was prevented by ALCAR in the liver and brain tissues. These results suggest that ALCAR is able to prevent the CCl4-induced oxidative stress.

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Acknowledgments

The authors thank the University Grants Commission-Special Assistance Program (UGC-SAP) for the instrumentation facility.

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  1. Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Thangaraj Annadurai, Shankaravadivelu Vigneshwari & Pitchairaj Geraldine

  2. Department of Biochemistry, K. S. R. College of Arts and Science, Thiruchengode, Namakkal, 637 215, Tamil Nadu, India

    Rajendran Thirukumaran

  3. Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirappalli, 620 001, Tamil Nadu, India

    Philip A. Thomas

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  1. Thangaraj Annadurai
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Correspondence to Pitchairaj Geraldine.

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Annadurai, T., Vigneshwari, S., Thirukumaran, R. et al. Acetyl-l-carnitine prevents carbon tetrachloride-induced oxidative stress in various tissues of Wistar rats. J Physiol Biochem 67, 519–530 (2011). https://doi.org/10.1007/s13105-011-0097-z

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