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Effect of dietary fiber of “Rihane” barley grains and azoxymethane on serum and liver lipid variables in Wistar rats

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Abstract

The aim of the present study is to evaluate the effects of diet enriched with dietary fiber of barley variety “Rihane” and azoxymethane on serum and liver lipid variables in male rats. Forty male rats were divided into four groups and fed on control diet or experimental diet that contained control enriched with dietary fiber of barley variety “Rihane”. Animals were injected with saline (controls) or azoxymethane (20 mg/kg body weight s.c.) at 7 and 8 weeks of age. The experimental diet significantly decreased cholesterol level compared with the control diet. Rats fed with BR diet significantly increased the serum high-density lipoprotein (HDL) cholesterol and significantly decreased low-density lipoprotein (LDL) cholesterol concentrations. The experimental diet decreased the atherogenic index (p < 0.05) compared with the control diet. Whereas the azoxymethane induced a significant increase of liver lipid, serum LDL and triglyceride concentrations, but it caused a significant reduction of HDL. Consequently, the ratio of HDL/TC decreased significantly compared with the control (p < 0.05). Accordingly, these results indicated that the diet enriched with dietary fiber of barley variety “Rihane” could be effective in decreasing the atherogenic risk factors in rats whereas the use of the azoxymethane as colon-specific carcinogen substance altered the lipid metabolism.

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Abbreviations

ADF:

Acid detergent fiber

ADL:

Acid detergent lignin

AOM:

Azoxymethane

BR:

Barley “Rihane”

BW:

Body weight

CVD:

Cardiovascular disease

CHD:

Coronary heart disease

DF:

Dietary fiber

DMH:

1,2-dimethylhydrazine

FDA:

Food and Drug Administration

HDL:

High-density lipoprotein-cholesterol

LDL:

Low-density lipoprotein-cholesterol

MAM:

Methylazoxymethanol

TC:

Total cholesterol

TG:

Triglycerides

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Acknowledgments

The authors wish to thank the personnel of Industry of Animal Nutrition-Tunisia “Provital”.

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Authors and Affiliations

  1. Unité de recherche 03/UR/09-01 “Génome, Diagnostic Immunitaire et valorisation”, Institut Supérieur de Biotechnologie de Monastir, Rue Tahar Hadded, BP 74, Monastir, 5019, Tunisia

    Lamia Lahouar, Fatma Ghrairi & Lotfi Achour

  2. Laboratoire des Grandes Cultures, INRA-Tunisia, 2049, Ariana, Tunisia

    Mouldi El Felah

  3. Laboratoire des Productions Animales et Fourragères, INRAT, 2049, Ariana, Tunisia

    Hichem Ben Salem

  4. Laboratoire de Biochimie, Hôpital Farhat Hachad Sousse, Sousse, Tunisia

    Abdel Hedi Miled

  5. Laboratoire de Biochimie, UR 08/39 “Nutrition Humaine et Désordres Métaboliques”, Faculté de Médecine de Monastir, Monastir, Tunisia

    Mohamed Hammami

Authors
  1. Lamia Lahouar
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  2. Fatma Ghrairi
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  3. Mouldi El Felah
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  4. Hichem Ben Salem
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  5. Abdel Hedi Miled
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  6. Mohamed Hammami
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  7. Lotfi Achour
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Correspondence to Lamia Lahouar.

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Lahouar, L., Ghrairi, F., El Felah, M. et al. Effect of dietary fiber of “Rihane” barley grains and azoxymethane on serum and liver lipid variables in Wistar rats. J Physiol Biochem 67, 27–34 (2011). https://doi.org/10.1007/s13105-010-0045-3

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  • DOI: https://doi.org/10.1007/s13105-010-0045-3

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