The aim of the study was to find markers of high-risk cardioembolic etiology (HRCE) in patients with cryptogenic strokes (CS) through the analysis of intracranial clot by flow cytometry (FC). A prospective single-center study was designed including patients with large vessel occlusion strokes. The percentage of granulocytes, monocytes, lymphocytes, and monocyte-to-lymphocyte ratio (MLr) were analyzed in clots extracted after endovascular treatment (EVT) and in peripheral blood. Large arterial atherosclerosis (LAA) strokes and high-risk cardioembolic (HRCE) strokes were matched by demographics and acute reperfusion treatment data to obtain FC predictors for HRCE. Multilevel decision tree with boosting random forest classifiers was performed with each feature importance for HRCE diagnosis among CS. We tested the validity of the best FC predictor in a cohort of CS that underwent extensive diagnostic workup. Among 211 patients, 178 cases underwent per-protocol workup. The percentage of monocytes (OR 1.06, 95% CI 1.01–1.11) and MLr (OR 1.83, 95% CI 1.12–2.98) independently predicted HRCE diagnosis when LAA clots (n = 28) were matched with HRCE clots (n = 28). Among CS (n = 82), MLr was the feature with the highest weighted importance in the multilevel decision tree as a predictor for HRCE. MLr cutoff point of 1.59 yield sensitivity of 91.23%, specificity of 44%, positive predictive value of 78.79%, and negative predictive value of 68.75 for HRCE diagnosis among CS. MLr ≥ 1.6 in clot analysis predicted HRCE diagnosis (OR, 6.63, 95% CI 1.85–23.71) in a multivariate model adjusted for age. Clot analysis by FC revealed high levels of monocyte-to-lymphocyte ratio as an independent marker of cardioembolic etiology in cryptogenic strokes.
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The data that support the findings of this study are available on request from the corresponding author.
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All the members of the stroke units are thanked for their support in the study and Cristina Granes is thanked for conducting the statistical analysis.
This work was supported by “Project 355/C/2017, Fundació La Marató de TV3 in Strokes and traumatic spinal cord and brain injury, 2017 call of projects”. J.J. was supported by a Rio Hortega contract CM18/00253 from The Instituto de Salud Carlos III (Institute of Health Carlos III, Spain).
The study was approved by the Ethical Committee in line with the last Helsinki.
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Juega, J., Palacio-Garcia, C., Rodriguez, M. et al. Monocyte-to-Lymphocyte Ratio in Clot Analysis as a Marker of Cardioembolic Stroke Etiology. Transl. Stroke Res. 13, 949–958 (2022). https://doi.org/10.1007/s12975-021-00946-w