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Atherosclerosis-Related Circulating MicroRNAs as a Predictor of Stroke Recurrence

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Abstract

MicroRNAs (miRNAs) are short sequenced non-coding RNAs that posttranscriptionally regulate gene expression. We investigated circulating miRNA expression levels in acute stroke patients and its relationship with future vascular event. We included acute ischemic stroke patients who admitted to a university hospital between May 1, 2011, and July 31, 2012, and the patients with vascular risk factors but not incident stroke as controls. We collected 5 ml of venous blood, and circulating miRNA levels were evaluated by quantitative real-time polymerase chain reaction. Five miRNAs (miR-17, miR-21, miR-106a, miR-126, and miR-200b), which had been reported to be related to atherosclerosis, were measured. The levels of miRNAs were compared with the presence of acute stroke, vascular risk factors, stroke subtypes, and stroke recurrence after index stroke. A total of 120 patients were included in the study, with 83 acute stroke patients. The levels of miR-17 were significantly increased in acute stroke patients, and the levels of miR-126 had positive correlation with cerebral atherosclerosis (r = 0.254, p = 0.021). Among the 83 stroke patients, eight experienced stroke recurrence during follow-up and higher level of miR-17 was associated with shorter event-free survival (p = 0.047). This study shows that the miR-17 level was elevated in acute ischemic stroke and associated with future stroke recurrence.

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Funding

This work was supported by a grant of the Korea Health technology R&D Project, Ministry of Health and Welfare, Republic of Korea (A111393). The funding has no role in design, collection, analysis, or interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication.

Ethical Approval

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional: Seoul National University Hospital, 0906-028-282) and with the Helsinki Declaration of 1975, as revised in 2008. Informed consent was obtained from all patients for being included in the study.

Conflict of Interest

Jeong-Min Kim, Keun-Hwa Jung, Kon Chu, Soon-Tae Lee, Jaejun Ban, Jangsup Moon, Manho Kim, Sang Kun Lee, and Jae-Kyu Roh, MD, PhD declare that they have no conflict of interest.

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    Authors and Affiliations

    1. Laboratory for Neurotherapeutics, Biomedical Research Institute, College of Medicine, Seoul National University, Seoul, South Korea

      Jeong-Min Kim, Keun-Hwa Jung, Kon Chu, Soon-Tae Lee, Jaejun Ban, Jangsup Moon, Manho Kim, Sang Kun Lee & Jae-Kyu Roh

    2. Department of Neurology, Chung-Ang University Medical Center, College of Medicine, Chung-Ang University, Seoul, South Korea

      Jeong-Min Kim

    3. Department of Neurology, Seoul National University Hospital, College of Medicine, Seoul National University, 28, Yongon-dong, Chongro-gu, Seoul, 110-744, South Korea

      Keun-Hwa Jung, Kon Chu, Soon-Tae Lee, Jangsup Moon, Manho Kim & Sang Kun Lee

    4. Department of Neurology, The Armed Forces Capital Hospital, 177, Saemaeulro, Bundang-gu, Seongnam, Gyeunggido, 463-040, South Korea

      Jae-Kyu Roh

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    1. Jeong-Min Kim
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    Correspondence to Kon Chu or Jae-Kyu Roh.

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    Jeong-Min Kim and Keun-Hwa Jung contributed equally to this work.

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    Kim, JM., Jung, KH., Chu, K. et al. Atherosclerosis-Related Circulating MicroRNAs as a Predictor of Stroke Recurrence. Transl. Stroke Res. 6, 191–197 (2015). https://doi.org/10.1007/s12975-015-0390-1

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    • DOI: https://doi.org/10.1007/s12975-015-0390-1

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