Abstract
Prolonged translation arrest in post-ischemic hippocampal CA1 pyramidal neurons precludes translation of induced stress genes and directly correlates with cell death. We evaluated the regulation of mRNAs containing adenine- and uridine-rich elements (ARE) by assessing HuR protein and hsp70 mRNA nuclear translocation, HuR polysome binding, and translation state analysis of CA1 and CA3 at 8 h of reperfusion after 10 min of global cerebral ischemia. There was no difference between CA1 and CA3 at 8 h of reperfusion in nuclear or cytoplasmic HuR protein or hsp70 mRNA, or HuR polysome association, suggesting that neither mechanism contributed to post-ischemic outcome. Translation state analysis revealed that 28 and 58 % of unique mRNAs significantly different between 8hR and NIC, in CA3 and CA1, respectively, were not polysome-bound. There was significantly greater diversity of polysome-bound mRNAs in reperfused CA3 compared to CA1, and in both regions, ARE-containing mRNAs accounted for 4–5 % of the total. These data indicate that posttranscriptional ARE-containing mRNA regulation occurs in reperfused neurons and contributes to post-ischemic outcome. Understanding the differential responses of vulnerable and resistant neurons to ischemia will contribute to the development of effective neuroprotective therapies.
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Acknowledgments
This work was sponsored by NIH NINDS Grant No. NS057167 (D.J.D.), an NINDS Ruth Kirsten F30 Predoctoral Fellowship Grant No. NINDS NS063651 (J.J.S.), and a Thomas C. Rumble Fellowship, Wayne State University (J.T.J.).
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All institutional and national guidelines for the care and use of laboratory animals were followed.
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All authors declare no conflict of interest.
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Szymanski, J.J., Wang, H., Jamison, J.T. et al. HuR Function and Translational State Analysis Following Global Brain Ischemia and Reperfusion. Transl. Stroke Res. 4, 589–603 (2013). https://doi.org/10.1007/s12975-013-0273-2
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DOI: https://doi.org/10.1007/s12975-013-0273-2