Abstract
We developed a novel model of a rat embolic cerebral infarction with a quantifiable autologous arterial blood clot. The left femoral artery had 0.15 ml of blood withdrawn and mixed with 10 units of thrombin in 50 μl saline. After 30 min, the clot was suctioned into a 4-French polyvinyl chloride tube. A 24-gage catheter was inserted up through the internal carotid artery via the external carotid artery stump. The 1-cm clot, at a volume of 7.2 mm3, was pushed and inserted into the internal carotid artery via the catheter. After withdrawing the catheter, the ICA blood flow recovered. We checked neurological status after 24 h (neurological free was 15, and worst was 1) and measured the infarction volume by the TTC method. Twelve rats were examined, and five sham-operated rats were included. Two rats were not able to achieve an 80 % reduction in CBF. One rat died due to cerebral infarction. The success rate in producing infarction was 83 %. The total infarction volume was 368.5 mm3 ± 61.2 se. Median neurological score was 6. Hemorrhagic transformation was not detected. Sham-operated rats revealed no infarction and no neurological deficit. The volume of infarction correlated significantly with the neurological score. We conclude that this embolic stroke model is useful in producing a human, severe cardioembolic cerebral infarction.
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Acknowledgments
We thank Mark Inglin (University of Basel) for his editorial assistance. And this work was supported by JSPS KAKENHI grant number 40312491 for NS.
Compliance with Ethics Requirements
This work was supported by JSPS KAKENHI grant number 40312491 for NS. We have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.
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Shimamura, N., Matsuda, N., Kakuta, K. et al. A Model of Rat Embolic Cerebral Infarction with a Quantifiable, Autologous Arterial Blood Clot. Transl. Stroke Res. 4, 564–570 (2013). https://doi.org/10.1007/s12975-013-0262-5
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DOI: https://doi.org/10.1007/s12975-013-0262-5