Translational Stroke Research

, Volume 3, Issue 3, pp 364–368 | Cite as

Minocycline Prevents IL-6 Increase after Acute Ischemic Stroke

  • Jeffrey A. SwitzerEmail author
  • Andrea Sikora
  • Adviye Ergul
  • Jennifer L. Waller
  • David C. Hess
  • Susan C. Fagan
Brief Communication


Higher levels of the inflammatory biomarker interleukin-6 (IL-6) correlate with poor clinical outcome in acute ischemic stroke (AIS). Minocycline (MC) is a known anti-inflammatory agent; thus, the effect of MC on IL-6 in the first 24 h of AIS was investigated to determine potential anti-inflammatory activity. The Minocycline to Improve Neurologic Outcome in Stroke (MINOS) study was a non-randomized dose-escalation (3.0–10.0 mg/kg) trial of IV MC for AIS within 6 h of onset. Plasma IL-6 samples were collected prior to MC treatment at 1, 24, and 72 h and compared to those collected in a separate observational study of blood biomarkers in AIS. IL-6 levels were measured by commercially available ELISA kits. The lower limit of detection for IL-6 was 1 pg/ml. Sixty MINOS subjects and 29 non-MINOS subjects were enrolled, and there was no difference in baseline stroke severity. There was no significant difference in IL-6 level pre-MC treatment at 1, 24, or 72 h. However, the odds of a non-detectable IL-6 at 24 h in MINOS were 8.94 (95% CI 2.62–30.46) compared with non-MINOS subjects. It is likely that even low doses of MC have a potent systemic anti-inflammatory effect in AIS. Whether this results in improved outcome will be tested in a randomized clinical trial.


Acute cerebral infarction Emergency treatment of stroke Inflammation Interleukin-6 Biomarkers 



This study was supported by the NIH/NINDS grants (DCH, R01 NS055728; SCF, RO1 NS063965; and AE, R21 NS070239), AHA EIA (AE, 0740002N), and the MCG Intramural Grants Program Scientist Training Program to JAS.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jeffrey A. Switzer
    • 1
    Email author
  • Andrea Sikora
    • 4
  • Adviye Ergul
    • 2
    • 3
    • 4
    • 5
  • Jennifer L. Waller
    • 3
  • David C. Hess
    • 1
    • 4
  • Susan C. Fagan
    • 1
    • 4
    • 5
  1. 1.Department of NeurologyGeorgia Health Sciences UniversityAugustaUSA
  2. 2.Department of PhysiologyGeorgia Health Sciences UniversityAugustaUSA
  3. 3.Department of BiostatisticsGeorgia Health Sciences UniversityAugustaUSA
  4. 4.Program in Clinical and Experimental Therapeutics, College of PharmacyUniversity of GeorgiaAugustaUSA
  5. 5.Charlie Norwood VA Medical CenterAugustaUSA

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