Abstract
Fusarium head blight (FHB) caused by Fusarium spp. is an emerging destructive wheat disease in Ethiopia. This disease significantly reduces grain yield and quality leading to small, shriveled, lightweight kernels and subsequently contaminating the grain with mycotoxins, particularly deoxynivalenol (DON). Field experiments were conducted in Mareko district, southern Ethiopia during the 2020 and 2021 main cropping seasons to determine the impact of integrated wheat variety resistance and fungicide application against FHB development and associated DON. Three bread wheat varieties (Denda'a, Hidase, and Kingbird), two fungicides (propiconazole and tebuconazole), and untreated wheat were used as a treatment in a split-plot design arranged in three replications. The main plots received wheat varieties, while the subplots received fungicides. The effect of fungicides on the FHB severity index was highly significant. Propiconazole and tebuconazole spraying at the anthesis growth stage reduced FHB incidence, severity, Fusarium-damaged kernels (FDK), and DON contamination in harvested grain while increasing thousand seed weight and yield in all varieties. Kingbird variety sprayed with tebuconazole had low mean FHB disease severity (19.33 and 20.20%), disease progression (0.0065 and 0.0079 units day–1), and AUDPC (316.52 and 330.17%-days) in 2020 and 2021, respectively. High mean grain yields were also obtained from a variety of Kingbird sprayed with tebuconazole. Furthermore, fungicide efficacy was found to be higher in the Kingbird variety treated with tebuconazole, with a 67% DON contamination reduction compared to the control. Unsprayed plots of each variety had the highest disease severity, DON contamination, AUDPC, and the lowest grain yield in both cropping years. The efficacy of fungicides in reducing the FHB severity index and yield increase was lower in the susceptible variety (Denda’a). Grain yield was negatively correlated to severity index, FDK, and DON, whereas the latter traits were positively correlated among themselves. The study indicated that fungicide efficacy in reducing FHB, and increasing grain yield was higher in moderately resistant varieties than in susceptible ones. The results imply that integrating fungicide application at the anthesis growth stage with moderately resistant variety is a more effective management approach for reducing FHB and DON contamination in wheat, than either approach used alone, and increasing wheat production and food security in major wheat growing areas of Ethiopia.
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References
Anteneh A, Asrat D (2020) Wheat production and marketing in Ethiopia: review study. Cogent Food Agriculture 6(1):1–14
Bai G, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161
Blandino M, Minelli L, Reyneri A (2006) Strategies for the chemical control of Fusarium head blight: effect on yield, alveographic parameters and deoxynivalenol contamination in winter wheat grain. Eur J Agron 25(3):193–201
Chala A, Weinert J, Wolf GA (2003) An integrated approach to the evaluation of the efficacy of fungicides against Fusarium culmorum, the cause of head blight of wheat. J Phytopathol 151(11–12):673–678
CSA (Central Statistical Agency) (2021) The Federal Democratic Republic of Ethiopia Central Statistical Agency. Agricultural sample survey in 2020/2021 volume I Report on area and production of major crops (Private Peasant Holdings, Meher Season). Statistical Bulletin. 586. Adis Ababa, Ethiopia
D’Angelo DL, Bradley CA, Ames KA, Willyerd KT, Madden LV, Paul PA (2014) Efficacy of fungicide applications during and after anthesis against Fusarium head blight and deoxynivalenol in soft red winter wheat. Plant Dis 98(10):1387–1397
EC. 2007. Commission Regulation 1126/2007 of 28 September 2007 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Official Journal of the European Union, 255: 14-17
Edwards SG (2004) Influence of agricultural practices on Fusarium infection of cereals and subsequent contamination of grain by trichothecene mycotoxins. Toxicol Lett 153(1):29–35
Fernando WG, Miller JD, Seaman WL, Seifert K, Paulitz TC (2000) Daily and seasonal dynamics of airborne spores of Fusarium graminearum and other Fusarium species sampled over wheat plots. Can J Bot 78(4):497–505
Gea FJ, Navarro MJ, Tello JC (2005) Reduced sensitivity of the mushroom pathogen Verticillium fungicola to prochloraz-manganese in vitro. Mycol Res 109(6):741–745
Góral T, Stuper-Szablewska K, Buśko M, Boczkowska M, Walentyn-Góral D, Wiśniewska H, Perkowski J (2015) Relationships between genetic diversity and Fusarium toxin profiles of winter wheat cultivars. The Plant Pathology Journal 31(3):226–244
Grote U, Fasse A, Nguyen TT, Erenstein O (2021) Food security and the dynamics of wheat and maize value chains in Africa and Asia. FrontiersSustainable Food Syst 4:1–17
Gupta R, Meghwal M, Prabhakar PK (2021) Bioactive compounds of pigmented wheat (Triticum aestivum): Potential benefits in human health. Trends Food Sci Technol 110:240–252
Haidukowski M, Pascale M, Perrone G, Pancaldi D, Campagna C, Visconti A (2005) Effect of fungicides on the development of Fusarium head blight, yield, and deoxynivalenol accumulation in wheat inoculated under field conditions with Fusarium graminearum and Fusarium culmorum. J Sci Food Agric 85(2):191–198
Jones RK (2000) Assessments of Fusarium head blight of wheat and barley in response to fungicide treatment. Plant Dis 84(9):1021–1030
Kebede M, Adugna G, Hundie B (2021) Status of Fusarium head blight on wheat fields in Southwestern Ethiopia. Italian J Mycol 50:117–130
Keller MD, (2011). The Contribution of within-field Inoculum sources of Gibberella zeae to Fusarium head blight in winter wheat and barley (Doctoral dissertation, Virginia Tech).
Kumar A, Mishra VK, Vyas RP, Singh V (2011) Heterosis and combining ability analysis in bread wheat (Triticum aestivum L). J Plant Breed Crop Sci 3(10):209–217
Lehoczki–Krsjak, S., Szabó-Hevér, Á., Tóth, B., Kótai, C., Bartók, T., Varga, M., Farády, L. and Mesterházy, Á. 2010. Prevention of Fusarium mycotoxin contamination by breeding and fungicide application to wheat. Food Additives and Contaminants, 27 (5): 616-628
Madden, L.V. and Campbell, C.L. 1990. Nonlinear disease progress curves. In Epidemics of plant diseases (pp. 181–229). Springer, Berlin, Heidelberg.
McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81(12):1340–1348
McMullen M, Halley S, Schatz B, Meyer S, Jordahl J, Ransom J (2008) Integrated strategies for Fusarium head blight management in the United States. Cereal Res Commun 36:563–568
McMullen M, Bergstrom G, De Wolf E, Dill-Macky R, Hershman D, Shaner G, Van Sanford D (2012) A unified effort to fight an enemy of wheat and barley: Fusarium head blight. Plant Dis 96(12):1712–1728
Mengesha GG, Abebe SM, Lera ZT, Shertore MM, Fedilu KB, Tadesse YB, Mekonnen AA, Cheleko DC (2021) Integration of host resistance, fungicides, and spray frequencies for managing Fusarium head blight of bread wheat under field conditions in southern Ethiopia. Heliyon 7(9):1–13
Mesterhazy A, Bartok T, Lamper C (2003) Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficacy of fungicides for control of Fusarium head blight. Plant Dis 87(9):1107–1115
Miedaner T, Gang G, Geiger HH (1996) Quantitative-genetic basis of aggressiveness of 42 isolates of Fusarium culmorum for winter rye head blight. Plant Dis 80(5):500–504
MoANR (Ministry of Agriculture and Natural Resources) and EATA (Ethiopian Agricultural Transformation Agency) (2018) Crop production and development package. In: Amharic version. Ministry of Agriculture, pp 215
Nakajima T., 2010. Fungicides application against Fusarium head blight in wheat and barley for ensuring food safety. Fungicides, pp.139–156.
Netsanet, B., Getaneh, W., Tsegab, T., Berhanu, B. and Nigussie, H. 2020. Survey and surveillance of wheat rust in the major wheat-growing areas of Ethiopia during 2017–2019. pp 13–19. In: Eshetu, D. and Bedada, G. (eds), Proceedings on the Delivering Genetic Gain in Wheat (DGGW) Project Closing Workshop. Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia.
Nigussie A, Kedir A, Adisu A, Belay G, Gebrie D, Desalegn K (2015) Bread wheat production in small-scale irrigation users agro-pastoral households in Ethiopia: case of Afar and Oromia regional state. J Dev Agric Econ 7(4):123–130
Osborne LE, Stein JM (2007) Epidemiology of Fusarium head blight on small grain cereals. Int J Food Microbiol 119:103–108
Paul PA, McMullen MP, Hershman DE, Madden LV (2010) Meta-analysis of the effects of triazole-based fungicides on wheat yield and test weight as influenced by Fusarium head blight intensity. Phytopathology 100(2):160–171
Paulitz TC (1996) Diurnal release of ascospores by Gibberella Zeae in inoculated wheat plots. Plant Dis 80:674–678
Ramirez ML, Chulze S, Magan N (2004) Impact of environmental factors and fungicides on growth and deoxynivalenol production by Fusarium graminearum isolates from Argentinian wheat. Crop Prot 23(2):117–125
Ransom JK, McMullen MV (2008) Yield and disease control on hard winter wheat cultivars with foliar fungicides. Agron J 100(4):1130–1137
Schmale DG III, Leslie JF, Zeller KA, Saleh AA, Shields EJ, Bergstrom GC (2006) Genetic structure of atmospheric populations of Gibberella zeae. Phytopathology. 96(9):1021–1026
Shah L, Ali A, Yahya M, Zhu Y, Wang S, Si H, Rahman H, Ma C (2018) Integrated control of fusarium head blight and deoxynivalenol mycotoxin in wheat. Plant Pathol 67(3):532–548
Shiferaw B, Kassie M, Jaleta M, Yirga C (2014) Adoption of improved wheat varieties and impacts on household food security in Ethiopia. Food Policy 44:272–284
Sulyok M, Stadler D, Steiner D, Krska R (2020) Validation of an LC-MS/MS-based dilute-and-shoot approach for the quantification of > 500 mycotoxins and other secondary metabolites in food crops: Challenges and solutions. Anal Bioanal Chem 412(11):2607–2620
Sun HY, Zhu YF, Liu YY, Deng YY, Li W, Zhang AX, Chen HG (2014) Evaluation of tebuconazole for the management of Fusarium head blight in China. Australas Plant Pathol 43(6):631–638
Sutton JC (1982) Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Can J Plant Path 4(2):195–209
Tadesse W, Bishaw Z, Assefa S (2019) Wheat production and breeding in Sub-Saharan Africa: challenges and opportunities in the face of climate change. Inter J Climate Change Strategies Management 11(5):696–715
Torres AM, Palacios SA, Yerkovich N, Palazzini JM, Battilani P, Leslie JF, Logrieco AF, Chulze SN (2019) Fusarium head blight and mycotoxins in wheat: prevention and control strategies across the food chain. World Mycotoxin J 12(4):333–355
Wegulo SN, Bockus WW, Nopsa JH, De Wolf ED, Eskridge KM, Peiris KH, Dowell FE (2011) Effects of integrating cultivar resistance and fungicide application on Fusarium head blight and deoxynivalenol in winter wheat. Plant Dis 95(5):554–560
Willyerd KT, Li C, Madden LV, Bradley CA, Bergstrom GC, Sweets LE, McMullen M, Ransom JK, Grybauskas A, Osborne L, Wegulo SN (2012) Efficacy and stability of integrating fungicide and cultivar resistance to manage Fusarium head blight and deoxynivalenol in wheat. Plant Dis 96(7):957–967
Winch T (2007) Growing Food: A Guide to Food Production. Springer Science and Business Media. Dordrecht, Netherlands, pp 143–149
Yoshida M, Nakajima T, Tomimura K, Suzuki F, Arai M, Miyasaka A (2012) Effect of the timing of fungicide application on Fusarium head blight and mycotoxin contamination in wheat. Plant Dis 96(6):845–851
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14(6):415–421
Acknowledgements
The study was financially supported by the Southern Agricultural Research Institute (SARI). We thank Werabe Agricultural Research Center for providing transport and other facilities during plating and data collection. We also greatly acknowledge Mr. Muluneh Mekiso for his unreserved assistance during field management and data collection.
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Getahun, M., Fininsa, C., Mohammed, A. et al. Integrated management of wheat (Triticum aestivum L.) Fusarium head blight and deoxynivalenol contamination through host resistance and fungicide application in Ethiopia. J. Crop Sci. Biotechnol. 27, 195–209 (2024). https://doi.org/10.1007/s12892-023-00223-2
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DOI: https://doi.org/10.1007/s12892-023-00223-2