Skip to main content

Advertisement

Log in

Development of early flowering, short life-spanned jute (Corchorus spp.) mutant via ethyl methane sulfonate mutagenesis

  • Original Research
  • Published:
Journal of Crop Science and Biotechnology Aims and scope Submit manuscript

Abstract

Polyethene is a global environmental threat, whereas jute (Corchorus spp.) fiber is biodegradable, eco-friendly and can be used as a substitute for polyethene. Jute is a short-day plant and cultivated in March to July in Indian subcontinent for bast fiber production. This season-bound cultivation nature hampers continuous supply of bast fiber in the industry. In addition, its long cultivation period creates difficulties to accommodate other high-value crops. To address these issues, a short life span (early flowering) jute genotype is extremely felt in jute growing areas of the world. But, unfortunately such variety has not been developed yet through both conventional and biotechnological approach. Accordingly, we adopted chemical mutagenesis by establishing LD50 (Lethal Dose) of EMS (Ethyl Methane Sulfonate) at 150 mM with 4h’ incubation for Capsularis jute seed. A novel mutant was found named—Komola, which showed a shorter lifespan than its ancestor by flowering at 70 days instead of 120 days. In addition, the mutant's stem and petiole was coppery-red instead of green. Molecular analysis revealed four SNPs in PMIR1 (Plastid Movement Impaired 1 Related 1) and two clade deletions in ELF3 (Early Flowering 3) genes from stable M4 generation. Histochemical and biochemical analyses explained this genotype’s lower content of lignin. This mutant could be used as future breeding material for the development of year-round cultivable jute genotype along with offer up accommodation of other high-value agricultural crops in cropping pattern with a good source of year-round supply of bast fiber to the industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Al-Snafi AE (2016) The contents and pharmacological importance of Corchorus capsularis- a review. IOSR J Pharma 6(6):58–63

    CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Bailey TL, Johnson J, Grant CE, Noble WS (2015) The MEME suite. Nucleic Acids Res 43:39–49

    Article  CAS  Google Scholar 

  • Begum T, Kumar D (2014) Breeding for premature flowering resistant lines with improved fibre yield and quality in tossa jute (Corchorus olitorius L.). Bangladesh J Bot 43(1):9–17

    Article  Google Scholar 

  • Bharadwaj P, Beena MR, Sinha M, Kirti PB (2011) In vitro regeneration and optimization of conditions for Agrobacterium mediated transformation in jute Corchorus Capsularis. J Plant Biochem Biotech 20:39–46

    Article  Google Scholar 

  • Bharadwaj P, Beena MR, Sinha M, Kirti PB (2013) Studies on explant regeneration and protoplast culture from hypocotyl segments of jute Corchorus Capsularis L. J Plant Studies 2(1):27–35

    CAS  Google Scholar 

  • Bouché F, Lobet G, Tocquin P, Périlleux C (2016) FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana. Nucleic Acids Res 44:1167–1171

    Article  CAS  Google Scholar 

  • Chaudhuri SD, Jabbar MA (1962) Species crosses in the genus Corchorus (jute plants). Euphytica 11:61–64

    Article  Google Scholar 

  • Cheng JZ, Zhou YP, Lv TX, Xie CP, Tian CE (2017a) Research progress on the autonomous flowering time pathway in Arabidopsis. Physiol Mol Biol Plants 23:477–485

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cheng JZ, Zhou YP, Lv TX, Xie CP, Tian CE (2017b) Research progress on the autonomous flowering time pathway in Arabidopsis. Physiol Mol Biol Plants 23(3):477–485

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Choudhary SB, Chowdhury I, Singh RK, Pandey SP, Sharma HK et al (2017) Morphological, histobiochemical and molecular characterisation of low lignin phloem fibre (llpf) mutant of dark jute (Corchorus olitorius L.). Appl Biochem Biotechnol 183(3):980–992

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bullet 19:11–15

    Google Scholar 

  • Hu B, Jin J, Guo AY, Zhang H, Luo J, Gao G (2015) GSDS 2.0: an upgraded gene feature visualization server. Bioinformatics 31(8):1296–1297

    Article  PubMed  Google Scholar 

  • Hunter S, Apweiler R, Attwood TK, Bairoch A, Bateman A et al (2009) InterPro: the integrative protein signature database. Nucleic Acids Res. https://doi.org/10.1093/nar/gkn785

    Article  PubMed  PubMed Central  Google Scholar 

  • Iseri OD, Yurtcu E, Sahin FI, Haberal M (2013) Corchorus olitorius (jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77). Pharma Biol 51:766–770

    Article  Google Scholar 

  • Islam M (2019) Varietal advances of jute, kenaf and mesta crops in Bangladesh: a review. Int J Bioorganic Chem 4:24–41

    Article  Google Scholar 

  • Islam AS, Rashid A (1960) First successful hybridization between the two jute yielding species, Corchorus olitorius (Tossa) × C capsularis (White). Nature 185:258–259

    Article  Google Scholar 

  • Islam MS, Saito JA, Emdad EM, Ahmed B, Islam MM et al (2017) Comparative genomics of two jute species and insight into fibre biogenesis. Nat Plants 3:16223

    Article  PubMed  CAS  Google Scholar 

  • Jahan MS, Liu ZH, Wang H, Saeed A, Ni Y (2012) Solation and characterization of lignin from prehydrolysis liquor of kraft-based dissolving pulp production. Cell Chem Technol 46:261–267

    CAS  Google Scholar 

  • Kar CS, Kundu A, Sarkar D, Sinha MK, Mahapatra BS (2009) Genetic diversity in jute (Corchorus spp) and its utilization: a review. Indian J Agric Sci 79:575–586

    Google Scholar 

  • Khatun R, Islam MM, Al-Hussain M, Pervin N, Sultana K (2009) Performance study of newly developed jute variety BJRI Deshi-7 (BJC-2142). Int J Sustain Agril Tech 5(4):12–18

    Google Scholar 

  • Kuo-Chen C, Shen HB (2010) Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization. PLoS ONE 5:11335

    Article  CAS  Google Scholar 

  • Lifschitz E, Eshed Y (2006) Universal florigenic signals triggered by FT homologues regulate growth and flowering cycles in perennial day-neutral tomato. J Exp Bot 57:3405–3414

    Article  CAS  PubMed  Google Scholar 

  • Ludwig CH, Nist BJ, McCarthy JL (1964) The high resolution nuclear magnetic resonance spectroscopy of protons in acetylated lignin. J American Chem Soc 86:1196–1201

    Article  CAS  Google Scholar 

  • Mohd-Yusoff NF, Ruperao P, Tomoyoshi NE (2015) Scanning the effects of ethyl methane sulfonate on the whole genome of Lotus japonicus using second-generation sequencing analysis. G3 Genes 5(6):559–567

    Google Scholar 

  • Noriyuki S, Higa T, Kong SG, Wada M (2015) Plastid movement impaired1 and plastid movement impaired1-related1 Mediate photo relocation movements of both chloroplasts and nuclei. Plant Physiol 169(2):1155–1167

    Article  CAS  Google Scholar 

  • Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A et al (2016) Principle and application of plant mutagenesis in crop improvement: a review. Biotech Biotechnol Equip 30:1–16

    Article  CAS  Google Scholar 

  • Salehi H, Ransom C, Oraby HF, Seddighi Z, Sticklen MB (2005) Delay in flowering and increase in biomass of plants expressing the Arabidopsis floral repressor gene FLC (FLOWERING LOCUS C). J Plant Physiol 162:711–716

    Article  CAS  PubMed  Google Scholar 

  • Sarker RH, Amin A, Hossain G, Hoque M (2007) In vitro regeneration in three varieties of white jute (Corchorus capsularis L). Plant Tissue Cul Biotech 17:11–18

    Article  Google Scholar 

  • Savojardo C, Martelli PL, Fariselli P, Casadio R (2018) DeepSig: deep learning improves signal peptide detection. Bioinformatics 34(10):1690–1696

    Article  CAS  PubMed  Google Scholar 

  • Sengupta G, Palit P (2004) Characterization of a lignified secondary phloem fibre-deficient mutant of jute (Corchorus capsularis L). Ann Bot 93:211–220

    Article  PubMed  PubMed Central  Google Scholar 

  • Shafrin F, Ferdous AS, Sarkar SK, Ahmed R, Amin A et al (2017) Modification of monolignol biosynthetic pathway in jute: different gene, different consequence. Sci Rep 7:39984

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shim JS, Kubota A, Imaizumi T (2017) Circadian clock and photoperiodic flowering in Arabidopsis: CONSTANS is a hub for signal integration. Plant Physiol 173:5–15. https://doi.org/10.1104/pp.16.01327

    Article  CAS  PubMed  Google Scholar 

  • Shuvobrata M, Prosanta S, Karabi D, Swapan KD (2020) Fiber crop, jute improvement by using genomics and genetic engineering. Advancement in crop improvement techniques. Woodhead Publishing, pp 363–383

    Google Scholar 

  • Sinha MK (2004) Jute quality breeding by reducing lignin content. In: New directions for a diverse planet. Proceedings for the 4th international crop science congress, Brisbane, Australia, p 26

  • Sinha MK, Kar CS, Ramasubramanian T, Kundu A, Mahapatra BS (2011) Corchorus. In: Kole C (ed) Wild crop relatives: genomic and breeding resources. Springer, Berlin, Heidelberg, pp 29–61

    Chapter  Google Scholar 

  • Song JH, Murphy RJ, Narayan R, Davies GB (2009) Biodegradable and compostable alternatives to conventional plastics. Phil Trans R Soc B 364(1526):2127–2139. https://doi.org/10.1098/rstb.2008.0289

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Soppe WJ, Bentsink L, Koornneef M (1999) The early-flowering mutant efs is involved in the autonomous promotion pathway of Arabidopsis thaliana. Develop 126:4763–4770

    Article  CAS  Google Scholar 

  • Stanke M, Morgenstern B (2005) AUGUSTUS: a web server for gene prediction in eukaryotes that allows user-defined constraints. Nucleic Acids Res 33:W465–W467. https://doi.org/10.1093/nar/gki458

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Statistical Bulletin-2014 (Food and Agriculture Organization of the United Nations (2014) http://wwwfaoorg/fileadmin/templates/est/COMM_MARKETS_MONITORING/Jute_Hard_Fibres/Documents/STAT_BULL_2014pdf

  • Subudhi PK, Leon TD, Tapia R (2018) Genetic interaction involving photoperiod-responsive Hd1 promotes early flowering under long-day conditions in rice. Sci Rep 8:2081

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Summerscales J, Dissanayake N, Virk A, Hall W (2010) A review of bast fibres and their composites. Compos Part A Appl Sci 41:1336–1344

    Article  CAS  Google Scholar 

  • Swaminathan MS, Iyer RD, Sulbha K (1961) Morphology, cytology and breeding behaviour of hybrids between Corchorus olitorius and C capsularis. Curr Sci 30:67–68

    Google Scholar 

  • Till BJ, Burtner C, Comai L, Henikoff S (2004) Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res 32(8):2632–2641

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Viana VE, Camila P, Carlos B, Antonio CO (2019) Mutagenesis in rice: the basis for breeding a new super plant. Front Plant Sci 10:1664–2462

    Article  Google Scholar 

  • Zhang G, Qi J, Xu J, Niu X, Zhang Y et al (2013) Overexpression of UDP-glucose pyrophosphorylase gene could increase cellulose content in jute (Corchorus capsularis L.). Biochem Biophys Res Commun 442(3–4):153–158

    Article  CAS  PubMed  Google Scholar 

  • Zhang L, Ming R, Zhang J, Tao A, Fang P, Qi J (2015) De novo transcriptome sequence and identification of major bast-related genes involved in cellulose biosynthesis in jute (Corchorus capsularis L). BMC Genomics 16:1062

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Zhang L, Ibrahim AK, Niyitanga S, Zhang L, Qi J (2019) Jute (Corchorus spp) Breeding. In: Al-Khayri J, Jain S, Johnson D (eds) Advances in plant breeding strategies: industrial and food crops. Springer, Cham Switzerland, pp 85–113

    Chapter  Google Scholar 

Download references

Acknowledgements

The author highly grateful to Dr. Borhan Ahmed, Rasel Ahmed, Md. Sabbir Hossen, Kazi Khayrul Bashar, and Md. Ruhul Amin for their help during the field research and molecular biological experiment.

Author information

Authors and Affiliations

Authors

Contributions

QMMH, SMBR, and MNR designed the study. QMMH, MDHS, MM, and MZT conducted field works and collected data. QMMH conducted the molecular experimentation and analysed the data. MSJ, KMYA, and QMMH conducted biochemical and histochemical experimentation. QMMH, MSH, SMBR, MNR, and MAS worked on the manuscript.

Corresponding author

Correspondence to Quazi Md. Mosaddeque Hossen.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 14 KB)

Supplementary file2 (DOCX 18 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hossen, Q.M.M., Rahman, S.M.B., Rahman, M.N. et al. Development of early flowering, short life-spanned jute (Corchorus spp.) mutant via ethyl methane sulfonate mutagenesis. J. Crop Sci. Biotechnol. 25, 489–500 (2022). https://doi.org/10.1007/s12892-022-00146-4

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12892-022-00146-4

Keywords

Navigation